Three dimensional OCT images from retina and skin.

We demonstrate the functionality of an en-face optical coherence tomography (OCT) system with images from the retina and skin. En-face images collected at different depths are subsequently used to reconstruct a 3D volume of the tissue. The reconstruction allows software inferred OCT longitudinal images at any transversal position in the stack. The position in depth in the stack before creating longitudinal OCT images is also adjustable, offering a valuable guidance tool for exploring the 3D volume of the tissue. This is illustrated by Quick time movies showing either depth or lateral exploration along one of two possible different directions in the stack of transversal OCT images. Sufficient accuracy of the volume rendered is obtained in 20 seconds when the system operates at 2 frames a second. The system, equipped with the 3D rendering feature acts as a valuable diagnostic tool allowing "peeling off" of transversal and longitudinal biologic material to investigate different internal features.

[1]  David A. Jackson,et al.  En-face coherence imaging using galvanometer scanner modulation. , 1998, Optics letters.

[2]  J. Izatt,et al.  Three-Dimensional Reconstruction of Blood Vessels from in vivo Color Doppler Optical Coherence Tomography Images , 1999, Dermatology.

[3]  L. Giniu̅nas,et al.  Scanning delay line with a rotating-parallelogram prism for low-coherence interferometry. , 1999, Applied optics.

[4]  David A. Jackson,et al.  OCT en-face images from the retina with adjustable depth resolution in real time , 1999 .

[5]  D L Farkas,et al.  Noninvasive imaging of living human skin with dual-wavelength optical coherence tomography in two and three dimensions. , 1998, Journal of biomedical optics.

[6]  J M Schmitt,et al.  Subsurface imaging of living skin with optical coherence microscopy. , 1995, Dermatology.

[7]  Adolf Friedrich Fercher,et al.  Dispersion induced multiple signal peak splitting in partial coherence interferometry , 1998 .

[8]  D. Jackson,et al.  Coherence imaging by use of a Newton rings sampling function. , 1996, Optics letters.

[9]  B Masters Three-dimensional confocal microscopy of the human optic nerve in vivo. , 1998, Optics express.

[10]  D. Davies,et al.  Optical coherence-domain reflectometry: a new optical evaluation technique. , 1987, Optics letters.

[11]  Tracy Stoudemayer,et al.  Optical coherence tomography in dermatology , 1999 .

[12]  J. Fujimoto,et al.  Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[13]  R. Webb,et al.  Video-rate confocal scanning laser microscope for imaging human tissues in vivo. , 1999, Applied optics.

[14]  D. Jackson,et al.  Noise analysis of a combined optical coherence tomograph and a confocal scanning ophthalmoscope. , 1999, Applied optics.

[15]  A Rollins,et al.  In vivo video rate optical coherence tomography. , 1998, Optics express.

[16]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991 .

[17]  David J. Webb,et al.  Transversal and longitudinal images from the retina of the living eye using low coherence reflectometry. , 1998, Journal of biomedical optics.

[18]  A. Podoleanu Unbalanced versus balanced operation in an optical coherence tomography system. , 2000, Applied optics.

[19]  S. Yazdanfar,et al.  An Optical Coherence Microscope for 3-dimensional Imaging in Developmental Biology References and Links , 2022 .

[20]  S Burns,et al.  Scanning laser reflectometry of retinal and subretinal tissues. , 2000, Optics express.