Optimization of solid-state fermentation for bioherbicide production by Phoma sp .

In this work the bioherbicide production from Phoma sp. by solid-state fermentation was optimized. Agroindustrial residues such as bagasse, soybean bran and corn steep liquor were used as substrate. The bioherbicide was extracted from the fermented solid and the supernatant was applied for the control of the target plant. The evaluated responses in the bioassay were plant height, root length, fresh and dry weight, number of flowers and phytotoxicity. The results in the bioassays demonstrated that the bioherbicide presented activity towards the target plant and the intensity of the effect was influenced by the formulation of the fermentation medium. The optimized condition for bioherbicide production was (wt%): moisture content 70.0, soybean bran content 30.0 and corn steep liquor (CSL) 20.0, it being possible to obtain an injury phytotoxicity level of 40. The bioherbicide showed a mode of action based on the inhibition of carotenoid biosynthesis.

[1]  R. Wilkinson.  Research Methods in Weed Science , 2016 .

[2]  M. Mazutti,et al.  An overview regarding bioherbicide and their production methods by fermentation , 2015 .

[3]  J. Glassey,et al.  Solid State Fermentation , 2015 .

[4]  Jerry M. Green,et al.  Current state of herbicides in herbicide-resistant crops. , 2014, Pest management science.

[5]  Jin-Ming Gao,et al.  New insight into the stereochemistry of botryosphaeridione from a Phoma endophyte , 2012 .

[6]  K. Bailey,et al.  The effects of Phoma macrostoma on nontarget plant and target weed species , 2011 .

[7]  S. Duke,et al.  Modes of Action of Microbially-Produced Phytotoxins , 2011, Toxins.

[8]  Rajeev K Sukumaran,et al.  Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases , 2010 .

[9]  G. Ash.  The science, art and business of successful bioherbicides , 2010 .

[10]  A. C. Monteiro,et al.  Crescimento e esporulação de Bipolaris euphorbiae cultivado sob diferentes condições nutricionais , 2008 .

[11]  Stephen O Duke,et al.  Natural products that have been used commercially as crop protection agents. , 2007, Pest management science.

[12]  H. Abbas,et al.  BIOHERBICIDES: RESEARCH AND RISKS , 2007 .

[13]  Naresh Magan,et al.  Medium optimization for the production of the secondary metabolite squalestatin S1 by a Phoma sp. combining orthogonal design and response surface methodology , 2005 .

[14]  J. Barton.  How good are we at predicting the field host-range of fungal pathogens used for classical biological control of weeds? , 2004 .

[15]  K. Bailey,et al.  Plant colonization and environmental fate of the biocontrol fungus Phoma macrostoma , 2004 .

[16]  J. Mitchell.  Development of a submerged-liquid sporulation medium for the potential smartweed bioherbicide Septoria polygonorum , 2003 .

[17]  E. Rosskopf,et al.  Biological weed control with pathogens: Search for candidates to applications , 2002 .

[18]  A. Dinoor,et al.  Biological control of weeds using plant pathogens: accomplishments and limitations , 2000 .

[19]  E. Rosskopf,et al.  CHAPTER 35 – Use of Plant Pathogens in Weed Control , 1999 .