Endocrine modulation and toxic effects of two commonly used UV screens on the aquatic invertebrates Potamopyrgus antipodarum and Lumbriculus variegatus.

The two UV screens 3-benzylidene-camphor (3-BC) and 3-(4'-methylbenzylidene)-camphor (4-MBC) were tested regarding their toxicity and estrogenic activity. The Yeast Estrogen Screen (YES) and two sediment assays with the freshwater invertebrates Lumbriculus variegatus and Potamopyrgus antipodarum were performed. In the YES, both substances activated the human estrogen receptor alpha with EC50 values of 44.2 microM for 3-BC and 44.3 microM for 4-MBC, whereby 4-MBC attained only 8% of the maximal response of 17beta-estradiol. For P. antipodarum embryo production increased after exposure to both substances (EC50 of 4.60 microM 4-MBC=1.17 mg kg(-1)dw) while mortality increased at high concentrations. The reproduction of L. variegatus was decreased by 3-BC with an EC50 of 5.95 microM (=1.43 mg kg(-1)dw) and also by 4-MBC, where no EC50 could be calculated. While reproduction decreased, the worms' weight increased after exposure to 3-BC with an EC50 of 26.9 microM (=6.46 mg kg(-1) dw), hence the total biomass remained unaffected.

[1]  J. Oehlmann,et al.  Reproductive stimulation by low doses of xenoestrogens contrasts with the view of hormesis as an adaptive response , 2005, Human & experimental toxicology.

[2]  L. Patrolecco,et al.  Uptake and accumulation of sediment‐associated 4‐nonylphenol in a benthic invertebrate (Lumbriculus variegatus, freshwater oligochaete) , 2005, Environmental toxicology and chemistry.

[3]  T. Poiger,et al.  Stereoisomer composition of the chiral UV filter 4-methylbenzylidene camphor in environmental samples. , 2005, Environmental science & technology.

[4]  Edwin J. Routledge,et al.  Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen , 1996 .

[5]  Risheng Ma,et al.  Estrogenic activity and estrogen receptor beta binding of the UV filter 3-benzylidene camphor. Comparison with 4-methylbenzylidene camphor. , 2004, Toxicology.

[6]  W. Verstraete,et al.  Optimization of a yeast estrogen screen and its applicability to study the release of estrogenic isoflavones from a soygerm powder. , 2001, Environmental health perspectives.

[7]  T. Poiger,et al.  Occurrence of some organic UV filters in wastewater, in surface waters, and in fish from Swiss Lakes. , 2005, Environmental science & technology.

[8]  W. Ponder.  Potamopyrgus antipodarum ― a molluscan coloniser of Europe and Australia , 1988 .

[9]  R. O. Hall,et al.  Extremely high secondary production of introduced snails in rivers. , 2006, Ecological applications : a publication of the Ecological Society of America.

[10]  G. Orphanides,et al.  Confirmation of uterotrophic activity of 3-(4-methylbenzylidine)camphor in the immature rat. , 2002, Environmental health perspectives.

[11]  J. Kukkonen,et al.  Bioaccumulation and toxicity of 4-nonylphenol (4-NP) and 4-(2-dodecyl)-benzene sulfonate (LAS) in Lumbriculus variegatus (Oligochaeta) and Chironomus riparius (Insecta). , 2006, Aquatic toxicology.

[12]  C. Wallace.  NOTES ON THE OCCURRENCE OF MALES IN POPULATIONS OF POTAMOPYRGUS JENKINSI , 1979 .

[13]  H. Galicia,et al.  Comparison of in vitro and in vivo estrogenic activity of UV filters in fish. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[14]  N. Skakkebaek,et al.  Systemic absorption of the sunscreens benzophenone-3, octyl-methoxycinnamate, and 3-(4-methyl-benzylidene) camphor after whole-body topical application and reproductive hormone levels in humans. , 2004, The Journal of investigative dermatology.

[15]  C. A. Harris,et al.  Issues arising when interpreting results from an in vitro assay for estrogenic activity. , 2000, Toxicology and applied pharmacology.

[16]  M. Schlumpf,et al.  Estrogen target gene regulation and coactivator expression in rat uterus after developmental exposure to the ultraviolet filter 4-methylbenzylidene camphor. , 2005, Endocrinology.

[17]  M. Liebig,et al.  Bioaccumulation of 14C-17alpha-ethinylestradiol by the aquatic oligochaete Lumbriculus variegatus in spiked artificial sediment. , 2005, Chemosphere.

[18]  G. Ankley,et al.  Use of the aquatic oligochaete Lumbriculus variegatus for assessing the toxicity and bioaccumulation of sediment‐associated contaminants , 1993 .

[19]  Bart van der Burg,et al.  Interaction of polycyclic musks and UV filters with the estrogen receptor (ER), androgen receptor (AR), and progesterone receptor (PR) in reporter gene bioassays. , 2004, Toxicological sciences : an official journal of the Society of Toxicology.

[20]  T. Poiger,et al.  Occurrence of UV filter compounds from sunscreens in surface waters: regional mass balance in two Swiss lakes. , 2004, Chemosphere.

[21]  C. Tyler,et al.  Comparative responses of molluscs and fish to environmental estrogens and an estrogenic effluent. , 2003, Aquatic toxicology.

[22]  J. Metzger,et al.  Determination of estrogenic activity by LYES-assay (yeast estrogen screen-assay assisted by enzymatic digestion with lyticase). , 2004, Chemosphere.

[23]  J. Oehlmann,et al.  Stimulated embryo production as a parameter of estrogenic exposure via sediments in the freshwater mudsnail Potamopyrgus antipodarum. , 2003, Aquatic toxicology.

[24]  H. Inui,et al.  Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes). , 2003, Toxicology.

[25]  R. Blust,et al.  Oestrogenic activity of CPRG (chlorophenol red-β-D-galactopyranoside), a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays. , 2001 .

[26]  T. Ternes,et al.  Effects of Pharmaceuticals on Aquatic Invertebrates. Part I. The Antiepileptic Drug Carbamazepine , 2005, Archives of environmental contamination and toxicology.

[27]  Bernd Markert,et al.  Effects of Endocrine Disruptors on Prosobranch Snails (Mollusca: Gastropoda) in the Laboratory. Part I: Bisphenol A and Octylphenol as Xeno-Estrogens , 2000, Ecotoxicology.

[28]  F. Urbach.  The historical aspects of sunscreens. , 2001, Journal of photochemistry and photobiology. B, Biology.

[29]  M. Neiman.  Embryo production in a parthenogenetic snail (Potamopyrgus antipodarum) is negatively affected by the presence of other parthenogenetic females , 2006 .

[30]  V. Forbes,et al.  Clonal variation in life‐history traits and feeding rates in the gastropod, Potamopyrgus antipodarum: performance across a salinity gradient , 1997 .

[31]  J. Oehlmann,et al.  Evidence for endocrine disruption in invertebrates. , 2004, International review of cytology.

[32]  T. Ternes,et al.  UV-Filtersubstanzen in Wasser und Fischen , 1997 .

[33]  Kristina A Thayer,et al.  Large effects from small exposures. I. Mechanisms for endocrine-disrupting chemicals with estrogenic activity. , 2003, Environmental health perspectives.

[34]  B. Kerans,et al.  Potamopyrgus antipodarum: distribution, density, and effects on native macroinvertebrate assemblages in the Greater Yellowstone Ecosystem , 2005, Journal of the North American Benthological Society.

[35]  David Crews,et al.  Resurrecting the Ancestral Steroid Receptor: Ancient Origin of Estrogen Signaling , 2003, Science.

[36]  B. Korsgaard,et al.  The chemical UV-filter 3-benzylidene camphor causes an oestrogenic effect in an in vivo fish assay. , 2002, Pharmacology & toxicology.

[37]  B. van der Burg,et al.  Estrogenic activity of UV filters determined by an in vitro reporter gene assay and an in vivo transgenic zebrafish assay , 2002, Archives of Toxicology.

[38]  S. Hansen,et al.  Distribution of the UV filter 3-benzylidene camphor in rat following topical application. , 2006, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[39]  J. Oehlmann,et al.  Prosobranch snails as test organisms for the assessment of endocrine active chemicals––an overview and a guideline proposal for a reproduction test with the freshwater mudsnail Potamopyrgus antipodarum , 2007, Ecotoxicology.

[40]  M. Schlumpf,et al.  In vitro and in vivo estrogenicity of UV screens. , 2001, Environmental health perspectives.

[41]  Peter Schmid,et al.  Occurrence of UV filters 4-methylbenzylidene camphor and octocrylene in fish from various Swiss rivers with inputs from wastewater treatment plants. , 2006, Environmental science & technology.

[42]  S. O. Mueller,et al.  Activation of estrogen receptor α and ERβ by 4-methylbenzylidene-camphor in human and rat cells: comparison with phyto- and xenoestrogens , 2003 .

[43]  J. Oehlmann,et al.  Toxicity of triphenyltin and tributyltin to the freshwater mud snail Potamopyrgus antipodarum in a new sediment biotest , 2003, Environmental toxicology and chemistry.