How Ca2+-ATPase pumps ions across the sarcoplasmic reticulum membrane.

Ca2+-ATPase of muscle sarcoplasmic reticulum is an ATP-powered Ca2+-pump that establishes a >10,000 fold concentration gradient across the membrane. Its crystal structures have been determined for 9 different states that cover nearly the entire reaction cycle. Presented here is a brief structural account of the ion pumping process, with emphasis on why the structure has to be so.

[1]  Y. Sugita,et al.  Protonation of the acidic residues in the transmembrane cation-binding sites of the ca(2+) pump. , 2005, Journal of the American Chemical Society.

[2]  J. Glusker.  Structural aspects of metal liganding to functional groups in proteins. , 1991, Advances in protein chemistry.

[3]  P. Nissen,et al.  Crystal structure of the sodium–potassium pump , 2007, Nature.

[4]  C. Oxvig,et al.  The structural basis of calcium transport by the calcium pump , 2007, Nature.

[5]  Poul Nissen,et al.  Phosphoryl Transfer and Calcium Ion Occlusion in the Calcium Pump , 2004, Science.

[6]  Hiroshi Suzuki,et al.  Distinct Natures of Beryllium Fluoride-bound, Aluminum Fluoride-bound, and Magnesium Fluoride-bound Stable Analogues of an ADP-insensitive Phosphoenzyme Intermediate of Sarcoplasmic Reticulum Ca2+-ATPase , 2004, Journal of Biological Chemistry.

[7]  Hiromi Nomura,et al.  Structural changes in the calcium pump accompanying the dissociation of calcium , 2002, Nature.

[8]  G. Inesi.  Sequential mechanism of calcium binding and translocation in sarcoplasmic reticulum adenosine triphosphatase. , 1987, The Journal of biological chemistry.

[9]  C. Toyoshima,et al.  Structural aspects of ion pumping by Ca2+-ATPase of sarcoplasmic reticulum. , 2008, Archives of biochemistry and biophysics.

[10]  Michael Y. Galperin,et al.  The catalytic domain of the P-type ATPase has the haloacid dehalogenase fold. , 1998, Trends in biochemical sciences.

[11]  G. Inesi,et al.  Ca2+ Occlusion and Gating Function of Glu309 in the ADP-Fluoroaluminate Analog of the Ca2+-ATPase Phosphoenzyme Intermediate* , 2004, Journal of Biological Chemistry.

[12]  Y. Sugita,et al.  Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+). , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[13]  C. Toyoshima,et al.  Conformational Fluctuations of the Ca2+-ATPase in the Native Membrane Environment , 2008, Journal of Biological Chemistry.

[14]  C. Toyoshima,et al.  Functional and structural roles of critical amino acids within the"N", "P", and "A" domains of the Ca2+ ATPase (SERCA) headpiece. , 2005, Biochemistry.

[15]  P. Nissen,et al.  Modulatory and catalytic modes of ATP binding by the calcium pump , 2006, The EMBO journal.

[16]  C. Toyoshima,et al.  How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump , 2007, Proceedings of the National Academy of Sciences.

[17]  Hiromi Nomura,et al.  Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues , 2004, Nature.

[18]  M. Saraste,et al.  FEBS Lett , 2000 .

[19]  S. Ebashi,et al.  ADENOSINE TRIPHOSPHATE-LINKED CONCENTRATION OF CALCIUM IONS IN A PARTICULATE FRACTION OF RABBIT MUSCLE , 1962, The Journal of cell biology.

[20]  Alessandro Senes,et al.  The Cα—H⋅⋅⋅O hydrogen bond: A determinant of stability and specificity in transmembrane helix interactions , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Andersen,et al.  Mutational Analysis of the Conserved TGES Loop of Sarcoplasmic Reticulum Ca2+-ATPase* , 2006, Journal of Biological Chemistry.

[22]  P. Champeil,et al.  Effect of magnesium on the calcium-dependent transient kinetics of sarcoplasmic reticulum ATPase, studied by stopped flow fluorescence and phosphorylation. , 1983, The Journal of biological chemistry.

[23]  C. Toyoshima,et al.  Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors , 2007, Proceedings of the National Academy of Sciences.

[24]  M. Picard,et al.  Functional Properties of Sarcoplasmic Reticulum Ca2+-ATPase after Proteolytic Cleavage at Leu119-Lys120, Close to the A-domain , 2004, Journal of Biological Chemistry.

[25]  L. DeMeis,et al.  Energy interconversion by the Ca2+-dependent ATPase of the sarcoplasmic reticulum. , 1979 .

[26]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[27]  Hiroshi Suzuki,et al.  Critical Role of Glu40-Ser48 Loop Linking Actuator Domain and First Transmembrane Helix of Ca2+-ATPase in Ca2+ Deocclusion and Release from ADP-insensitive Phosphoenzyme* , 2007, Journal of Biological Chemistry.

[28]  G. Inesi,et al.  Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles. , 1980, The Journal of biological chemistry.

[29]  Hiroshi Suzuki,et al.  Deletions of Any Single Residues in Glu40-Ser48 Loop Connecting A Domain and the First Transmembrane Helix of Sarcoplasmic Reticulum Ca2+-ATPase Result in Almost Complete Inhibition of Conformational Transition and Hydrolysis of Phosphoenzyme Intermediate* , 2003, Journal of Biological Chemistry.

[30]  A. P. Einholm,et al.  Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum Ca2+-ATPase assists in catalysis of E2/E2P partial reactions. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[31]  C. Toyoshima,et al.  Crystal structure of the calcium pump with a bound ATP analogue , 2004, Nature.

[32]  P. Nissen,et al.  Dephosphorylation of the Calcium Pump Coupled to Counterion Occlusion , 2004, Science.

[33]  J. Møller,et al.  Structural organization, ion transport, and energy transduction of P-type ATPases. , 1996, Biochimica et biophysica acta.

[34]  C. Toyoshima,et al.  Organization of cytoplasmic domains of sarcoplasmic reticulum Ca2+‐ATPase in E1P and E1ATP states: a limited proteolysis study , 2001, FEBS letters.

[35]  R. Guidelli,et al.  Pre-steady State Electrogenic Events of Ca2+/H+ Exchange and Transport by the Ca2+-ATPase* , 2006, Journal of Biological Chemistry.

[36]  Hiroshi Suzuki,et al.  ADP‐insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+‐ATPase has a compact conformation resistant to proteinase K, V8 protease and trypsin , 2001, FEBS letters.

[37]  M. Nakasako,et al.  Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Å resolution , 2000, Nature.

[38]  M. Picard,et al.  Effects of Inhibitors on Luminal Opening of Ca2+ Binding Sites in an E2P-like Complex of Sarcoplasmic Reticulum Ca22+-ATPase with Be22+-fluoride* , 2006, Journal of Biological Chemistry.

[39]  C. Toyoshima,et al.  Structural basis of ion pumping by Ca2+-ATPase , 2004 .

[40]  W. Kühlbrandt.  Biology, structure and mechanism of P-type ATPases , 2004, Nature Reviews Molecular Cell Biology.

[41]  G. Inesi,et al.  Cooperative setting for long-range linkage of Ca(2+) binding and ATP synthesis in the Ca(2+) ATPase. , 2002, Biophysical journal.