Cranial and postcranial skeletal variations induced in mouse embryos by mobile phone radiation.

This study focuses on foetal development following mild daily exposure of pregnant mice to near field electromagnetic radiation emitted by a mobile phone. The investigation was motivated by the fact that the potentially hazardous electromagnetic radiation emitted by mobile phones is currently of tremendous public interest. Physically comparable pregnant mice were exposed to radiofrequency radiation GSM 900MHz emitted by a mobile phone. Within 5h after birth most cubs were fixed followed by double staining in toto, and conventional paraffin histology. Other cubs remained with their mothers until teeth eruption. Structural development was assessed by examining newborns for the presence of anomalies and/or variations in soft tissues and skeletal anatomy. Electromagnetic radiofrequency exposed newborns, externally examined, displayed a normal phenotype. Histochemical and histological studies, however, revealed variations in the exposed foetuses with respect to control ones concerning the ossification of cranial bones and thoracic cage ribs, as well as displacement of Meckelian cartilage. Littermates examined after teeth eruption displayed normal phenotypes. It is concluded that mild exposure to mobile phone radiation may affect, although transiently, mouse foetal development at the ossification level. The developmental variations observed could be explained by considering the different embryonic origin and mode of ossification of the affected skeletal elements.

[1]  Carl Blackman,et al.  Cell phone radiation: Evidence from ELF and RF studies supporting more inclusive risk identification and assessment. , 2009, Pathophysiology : the official journal of the International Society for Pathophysiology.

[2]  Alvin C. Harper,et al.  Mobile Telephones: Networks, Applications, and Performance , 2008 .

[3]  V. Jousmäki,et al.  Effects of 45-Hz magnetic fields on the functional state of the human brain. , 1993, Bioelectromagnetics.

[4]  M. E. Chernovetz,et al.  Teratology, survival, and reversal learning after fetal irradiation of mice by 2450-MHz microwave energy. , 1975, The Journal of microwave power.

[5]  Olle Johansson,et al.  Personal digital assistant (PDA) cell phone units produce elevated extremely‐low frequency electromagnetic field emissions , 2007 .

[6]  Bertil R. R. Persson,et al.  Blood-Brain Barrier Permeability and Nerve Cell Damage in Rat Brain 14 and 28 Days After Exposure to Microwaves from GSM Mobile Phones , 2008, Electromagnetic biology and medicine.

[7]  Ersan Odaci,et al.  Effects of prenatal exposure to a 900 MHz electromagnetic field on the dentate gyrus of rats: a stereological and histopathological study , 2008, Brain Research.

[8]  Leeka Kheifets,et al.  Prenatal and Postnatal Exposure to Cell Phone Use and Behavioral Problems in Children , 2008, Epidemiology.

[9]  Some Ocular Symptoms Experienced by Users of Mobile Phones , 2008, Electromagnetic biology and medicine.

[10]  V. Romano Spica,et al.  No evidence of major transcriptional changes in the brain of mice exposed to 1800 MHz GSM signal , 2008, Bioelectromagnetics.

[11]  J. Fröhlich,et al.  Blood-brain barrier and electromagnetic fields: effects of scopolamine methylbromide on working memory after whole-body exposure to 2.45 GHz microwaves in rats. , 2005, Behavioural brain research.

[12]  Antti Juvonen,et al.  Mobile Phone Radiation and the Developing Brain: Behavioral and Morphological Effects in Juvenile Rats , 2007, Radiation research.

[13]  J. Herbertz.  Comment on the ICNIRP guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz) , 1998, Health physics.

[14]  O. Fujiwara,et al.  FDTD calculation of whole-body average SAR in adult and child models for frequencies from 30 MHz to 3 GHz , 2006, Physics in medicine and biology.

[15]  James C Lin,et al.  Studies on microwaves in medicine and biology: From snails to humans , 2004, Bioelectromagnetics.

[16]  Cindy Sage,et al.  Biological effects from electromagnetic field exposure and public exposure standards. , 2008, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[17]  K. Khera.  Maternal toxicity: a possible etiological factor in embryo-fetal deaths and fetal malformations of rodent-rabbit species. , 1985, Teratology.

[18]  C Gabriel,et al.  Changes in the dielectric properties of rat tissue as a function of age at microwave frequencies. , 2001, Physics in medicine and biology.

[19]  R. Hilloowala,et al.  Interrelationships of brain, cranial base and mandible. , 1998, Cranio : the journal of craniomandibular practice.

[20]  V. Anderson,et al.  A numerical evaluation of SAR distribution and temperature changes around a metallic plate in the head of a RF exposed worker , 2005, Bioelectromagnetics.

[21]  C A Kimmel,et al.  A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. , 1981, Stain technology.

[22]  Ashok Agarwal,et al.  Cell phones: modern man's nemesis? , 2009, Reproductive biomedicine online.

[23]  R. Jensh,et al.  Teratologic studies of prenatal exposure of rats to 915 MHz microwave radiation. , 1982, Radiation research.

[24]  L A Opperman,et al.  Cranial sutures as intramembranous bone growth sites , 2000, Developmental dynamics : an official publication of the American Association of Anatomists.

[25]  T. Tsiboukis,et al.  Bone morphogenetic protein expression in newborn rat kidneys after prenatal exposure to radiofrequency radiation , 2004, Bioelectromagnetics.

[26]  Reilly Jp.  Comments concerning "Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz)". , 1999 .

[27]  K. Jokela,et al.  Specific absorption rate and electric field measurements in the near field of six mobile phone base station antennas , 2009, Bioelectromagnetics.

[28]  A. Hirata,et al.  Intercomparison of whole-body averaged SAR in European and Japanese voxel phantoms , 2008, Physics in medicine and biology.

[29]  M. Soley,et al.  Immobilization stress induces c-Fos accumulation in liver , 2000, Cell stress & chaperones.

[30]  Olle Johansson,et al.  Personal digital assistant (PDA) cell phone units produce elevated extremely‐low frequency electromagnetic field emissions , 2007, Bioelectromagnetics.

[31]  Belyaev Iy,et al.  Problems in assessment of risks from exposures to microwaves of mobile communication. , 2007 .

[32]  Lars Malmgren,et al.  Nerve cell damage in mammalian brain after exposure to microwaves from GSM mobile phones. , 2003, Environmental health perspectives.

[33]  L. S. Kinney,et al.  Effects of ELF fields on calcium-ion efflux from brain tissue in vitro. , 1982, Radiation research.

[34]  Matti Laine,et al.  Effects of 902 MHz electromagnetic field emitted by cellular telephones on response times in humans , 2000, Neuroreport.

[35]  Risto Myllylä,et al.  FM-radio and TV tower signals can cause spontaneous hand movements near moving RF reflector. , 2009, Pathophysiology : the official journal of the International Society for Pathophysiology.

[36]  R. Zaridze,et al.  Influence of Specific Absorption Rate Averaging Schemes on Correlation between Mass-Averaged Specific Absorption Rate and Temperature Rise , 2009 .

[37]  C. Chou.  Use of heating rate and specific absorption rate in the hyperthermia clinic. , 1990, International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group.

[38]  G. Hyland.  Physics and biology of mobile telephony , 2000, The Lancet.

[39]  R. Tyl,et al.  Altered axial skeletal development. , 2007, Birth defects research. Part B, Developmental and reproductive toxicology.

[40]  J. Fröhlich,et al.  Blood–brain barrier and electromagnetic fields: Effects of scopolamine methylbromide on working memory after whole-body exposure to 2.45GHz microwaves in rats , 2005, Behavioural Brain Research.

[41]  P. Dimbylow,et al.  Spatial averaging of fields from half-wave dipole antennas and corresponding SAR calculations in the NORMAN human voxel model between 65 MHz and 2 GHz. , 2009, Physics in medicine and biology.

[42]  I. Magras,et al.  RF radiation-induced changes in the prenatal development of mice. , 1997, Bioelectromagnetics.

[43]  R. Wallace,et al.  Reproductive and Developmental Effects , 2001 .

[44]  Elisabeth Cardis,et al.  Cellular phone use and risk of benign and malignant parotid gland tumors--a nationwide case-control study. , 2008, American journal of epidemiology.

[45]  Kanako Wake,et al.  Effects of gestational exposure to 1.95‐GHz W‐CDMA signals for IMT‐2000 cellular phones: Lack of embryotoxicity and teratogenicity in rats , 2009, Bioelectromagnetics.

[46]  D. Bamiou,et al.  Mobile telephone use effects on peripheral audiovestibular function: A case–control study , 2008, Bioelectromagnetics.

[47]  R. Jensh,et al.  Behavioral teratologic studies using microwave radiation: is there an increased risk from exposure to cellular phones and microwave ovens? , 1997, Reproductive toxicology.

[48]  J. Bausch,et al.  Reproductive safety studies with genistein in rats. , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[49]  B. Schwetz,et al.  Embryo- and fetotoxicity of inhaled carbon tetrachloride, 1,1-dichloroethane and methyl ethyl ketone in rats. , 1974, Toxicology and applied pharmacology.

[50]  Jong-Choon Kim,et al.  Developmental toxicity evaluation of ELF magnetic fields in Sprague–Dawley rats , 2003, Bioelectromagnetics.

[51]  H. B. Carter,et al.  Observations of mouse fetuses after irradiation with 2.45 GHz microwaves. , 1978, Health physics.