Separation of lymphocytes and macrophages from suspensions of guinea pig peritonitis exudate cells using programmed gradient sedimentation.

A vARIETY OF TISSUES AND CELL TYPES have been used to study the different aspects of delayed hypersensitivity in vitro.' In 1962, George and Vaughan2 introduced the macrophage inhibition test, an assay for delayed hypersensitivity in vitro involving the inhibition of peritonitis cell migration from capillary tubes. David et a13 utilized this technic to show that as few as 2.5% peritoneal exudate cells from a sensitized guinea pig inhibit the migration of a mixture of peritoneal cells from sensitized and "normal" animals. The macrophage inhibition test has been used to demonstrate sensitization to brain extracts in animals with experimental autoimmune (allergic) encephalomyelitis,4 in the detection of tumor-specific antigens,5 to prove immunity resulting from virus infection 6 and in a host of other experimental situations.7-9

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