Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue

Bioactive three-dimensional (3D) printed scaffolds are promising candidates for bone tissue engineering (BTE) applications. Here, we introduce a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs that resemble the organic and inorganic composition of native bone tissue. A uniform dispersion of BG particles within the collagen network improved stability and reduced swelling of collagen hydrogels. Rheological testing showed significant improvement in the yield stress and percent recovery of 3D printed constructs upon BG incorporation. Further, addition of BG improved the bone bioactivity of 3D printed constructs in stimulated body fluid. BG incorporated CMA (BG-CMA) constructs maintained high cell viability and enhanced alkaline phosphatase activity of human mesenchymal stem cells. In addition, cell-mediated calcium deposition was significantly higher on BG-CMA constructs, compared to CMA alone. In conclusion, 3D printed BG-CMA constructs have significant potential for use in BTE applications.

[1]  Vipuil Kishore,et al.  Dual crosslinking strategy to generate mechanically viable cell-laden printable constructs using methacrylated collagen bioinks. , 2020, Materials science & engineering. C, Materials for biological applications.

[2]  Guifeng Zhang,et al.  Hydroxyapatite/Collagen Three-Dimensional Printed Scaffolds and Their Osteogenic Effects on Human Bone Marrow-Derived Mesenchymal Stem Cells. , 2019, Tissue engineering. Part A.

[3]  T. L. Montanheiro,et al.  Current advances in bone tissue engineering concerning ceramic and bioglass scaffolds: A review , 2019 .

[4]  Jianzhong Fu,et al.  3D printing of biomimetic multi-layered GelMA/nHA scaffold for osteochondral defect repair , 2019, Materials & Design.

[5]  J. Massera,et al.  Wood-based nanocellulose and bioactive glass modified gelatin–alginate bioinks for 3D bioprinting of bone cells , 2019, Biofabrication.

[6]  Binghong Luo,et al.  3D bioprinting of gellan gum and poly (ethylene glycol) diacrylate based hydrogels to produce human-scale constructs with high-fidelity , 2018, Materials & Design.

[7]  Anthony Atala,et al.  Optimization of gelatin–alginate composite bioink printability using rheological parameters: a systematic approach , 2018, Biofabrication.

[8]  He Lian,et al.  Fabrication, characterization and osteoblast responses of poly (octanediol citrate)/bioglass nanofiber composites. , 2018, Materials science & engineering. C, Materials for biological applications.

[9]  Malcolm Xing,et al.  3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances , 2018, Bioactive materials.

[10]  Jianzhong Fu,et al.  3D Bioprinting of Low-Concentration Cell-Laden Gelatin Methacrylate (GelMA) Bioinks with a Two-Step Cross-linking Strategy. , 2018, ACS applied materials & interfaces.

[11]  Lawrence J Bonassar,et al.  Correlating rheological properties and printability of collagen bioinks: the effects of riboflavin photocrosslinking and pH , 2017, Biofabrication.

[12]  Geun Hyung Kim,et al.  An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues , 2017, Scientific Reports.

[13]  Aji P. Mathew,et al.  3D printing of nano-cellulosic biomaterials for medical applications , 2017 .

[14]  L. Garrido,et al.  Processing of concentrated aqueous zirconia-bioglass slips by slip casting , 2017 .

[15]  T. Scheibel,et al.  Strategies and Molecular Design Criteria for 3D Printable Hydrogels. , 2016, Chemical reviews.

[16]  Joon Hyung Park,et al.  Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels , 2015, Science Advances.

[17]  Zhen-an Zhu,et al.  Fabrication of three-dimensional porous scaffold based on collagen fiber and bioglass for bone tissue engineering. , 2015, Journal of biomedical materials research. Part B, Applied biomaterials.

[18]  Julian R. Jones.  Reprint of: Review of bioactive glass: From Hench to hybrids. , 2015, Acta biomaterialia.

[19]  T. Gill,et al.  Enhancing the stiffness of collagen hydrogels for delivery of encapsulated chondrocytes to articular lesions for cartilage regeneration. , 2015, Journal of biomedical materials research. Part A.

[20]  Werner E. G. Müller,et al.  Effect of Bioglass on Growth and Biomineralization of SaOS-2 Cells in Hydrogel after 3D Cell Bioprinting , 2014, PloS one.

[21]  Zeqiang He,et al.  Fabrication and Properties of Porous Collagen/Hyaluronic Composite Scaffolds Containing Nano-Bioactive Glass for Tissue Engineering , 2014 .

[22]  Anthony Atala,et al.  3D bioprinting of tissues and organs , 2014, Nature Biotechnology.

[23]  Changqing Zhang,et al.  Three-dimensional printing of strontium-containing mesoporous bioactive glass scaffolds for bone regeneration. , 2014, Acta biomaterialia.

[24]  E. Stone,et al.  Mechanical properties of murine and porcine ocular tissues in compression. , 2014, Experimental eye research.

[25]  H. Kim,et al.  Collagen hydrogels incorporated with surface-aminated mesoporous nanobioactive glass: Improvement of physicochemical stability and mechanical properties is effective for hard tissue engineering. , 2013, Acta biomaterialia.

[26]  A. Bandyopadhyay,et al.  Bone tissue engineering using 3D printing , 2013 .

[27]  J. Jansen,et al.  Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration: characterization and in vitro biological behavior. , 2013, Dental materials : official publication of the Academy of Dental Materials.

[28]  Aldo R. Boccaccini,et al.  Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites , 2013, Journal of Materials Science: Materials in Medicine.

[29]  D. Shreiber,et al.  Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel , 2012, Biointerphases.

[30]  G. Niebur,et al.  Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche. , 2012, European cells & materials.

[31]  Aldo R Boccaccini,et al.  Accelerated mineralization of dense collagen-nano bioactive glass hybrid gels increases scaffold stiffness and regulates osteoblastic function. , 2011, Biomaterials.

[32]  W. Lems,et al.  Advances in Glucocorticoid-Induced Osteoporosis , 2011, Current rheumatology reports.

[33]  Masoud Mozafari,et al.  Biomimetic formation of apatite on the surface of porous gelatin/bioactive glass nanocomposite scaffolds , 2010 .

[34]  Julian R. Jones,et al.  Differentiation of fetal osteoblasts and formation of mineralized bone nodules by 45S5 Bioglass conditioned medium in the absence of osteogenic supplements. , 2009, Biomaterials.

[35]  E. Harvey,et al.  Glucocorticoids in osteonecrosis of the femoral head: A new understanding of the mechanisms of action , 2009, The Journal of Steroid Biochemistry and Molecular Biology.

[36]  N. Pallua,et al.  Human Bone Marrow Mesenchymal Stem Cells Seeded on Modified Collagen Improved Dermal Regeneration In Vivo , 2006, Cell transplantation.

[37]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[38]  Tadashi Kokubo,et al.  How useful is SBF in predicting in vivo bone bioactivity? , 2006, Biomaterials.

[39]  C. Patrick,et al.  Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue. , 2005, Journal of biomedical materials research. Part A.

[40]  K. Leong,et al.  Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. , 2003, Biomaterials.

[41]  Cato T Laurencin,et al.  Three-dimensional, bioactive, biodegradable, polymer-bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro. , 2003, Journal of biomedical materials research. Part A.

[42]  Yubao Li,et al.  Development of biomimetic nano-hydroxyapatite/poly(hexamethylene adipamide) composites. , 2002, Biomaterials.

[43]  P Eiselt,et al.  Porous carriers for biomedical applications based on alginate hydrogels. , 2000, Biomaterials.

[44]  H. Oonishi,et al.  Particulate Bioglass Compared With Hydroxyapatite as a Bone Graft Substitute , 1997, Clinical orthopaedics and related research.

[45]  byBrooke LaBranche,et al.  3 D bioprinting of tissues and organs , 2017 .

[46]  Ibrahim T. Ozbolat,et al.  Current advances and future perspectives in extrusion-based bioprinting. , 2016, Biomaterials.

[47]  Aldo R Boccaccini,et al.  Combining Collagen and Bioactive Glasses for Bone Tissue Engineering: A Review , 2015, Advanced healthcare materials.

[48]  Amy J Wagoner Johnson,et al.  The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. , 2007, Biomaterials.

[49]  D Kaspar,et al.  Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. , 2005, Biomaterials.

[50]  A. Ignatiusa,et al.  Tissue engineering of bone : effects of mechanical strain on osteoblastic cells in type I collagen matrices , 2004 .