A numerical classification of some Thermus isolates

Summary: A numerical classification was performed on a collection of 45 Thermus isolates recovered from New Zealand hot pools and on six type strains including T. aquaticus and “T. thermophilus”. Unweighted average linking (UPGMA) and single linkage clustering methods were applied to similarity matrices derived from simple matching (SSM ) and Jaccard similarity (SJ ) coefficients. Differences were observed between phenograms derived from SSM and SJ coefficients, indicating that some of the clusters formed were derived from a significant component of negative matches. Test error was estimated at 2.9%. In the UPGMA/SSM phenogram, seven clusters were formed. A majority of the New Zealand isolates did not cluster with non-New Zealand isolates. Analysis of variance showed that there was a relationship between the composition of the clusters and the temperature and pH of the source of the isolate. Chi-squared testing showed that, within New Zealand, the geographical source of the isolate had no bearing on the clusters formed.

[1]  B. Patel,et al.  Thermophilic anaerobic spirochetes in New Zealand hot springs , 1985 .

[2]  L. G. Loginova,et al.  Thermus ruber sp. nov., nom. rev. , 1984 .

[3]  J. Kristjánsson,et al.  Distribution of Thermus spp. in Icelandic Hot Springs and a Thermal Gradient , 1983, Applied and environmental microbiology.

[4]  H. Matsuzawa,et al.  Heat-stable and fructose 1,6-bisphosphate-activated L-lactate dehydrogenase from an extremely thermophilic bacterium. , 1982, Journal of biochemistry.

[5]  T. D. Brock.  The Genus Thermus , 1978 .

[6]  R. Williams,et al.  Yellow-pigmented Strains of Thermus spp. from Icelandic Hot Springs , 1977 .

[7]  B. Wilkinson,et al.  A numerical taxonomic survey of Listeria and related bacteria. , 1977, Journal of general microbiology.

[8]  R. Ramaley,et al.  Isolation of a new, pink, obligately thermophilic, gram negative bacterium (K 2 isolate) , 1975 .

[9]  Loginova Lg,et al.  Thermus ruber obligate thermophilic bacteria in the thermal springs of Kamchatka , 1975 .

[10]  R. Williams,et al.  Extremely thermophilic gram-negative bacteria from hot tap water. , 1975, Journal of general microbiology.

[11]  K. Imahori,et al.  Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a Nonsporulating Thermophilic Bacterium from a Japanese Thermal Spa , 1974 .

[12]  T. D. Brock,et al.  Presence of thermophilic bacteria in laundry and domestic hot-water heaters. , 1973, Applied microbiology.

[13]  P H Sneath,et al.  The influence on numerical taxonomic similarities of errors in microbiological tests. , 1972, Journal of general microbiology.

[14]  K. Arima,et al.  Isolation and Characterization of Extremely Thermophilic Bacteria from Hot Springs , 1972 .

[15]  R. Ramaley,et al.  Isolation of a Nonpigmented, Thermophilic Bacterium Similar to Thermus aquaticus , 1970 .

[16]  H. Freeze,et al.  Thermus aquaticus gen. n. and sp. n., a Nonsporulating Extreme Thermophile , 1969, Journal of bacteriology.

[17]  A. C. Baird‐Parker.  The classification of fusobacteria from the human mouth. , 1960, Journal of general microbiology.

[18]  Robert R. Sokal,et al.  A statistical method for evaluating systematic relationships , 1958 .

[19]  P. Sneath.  The application of computers to taxonomy. , 1957, Journal of general microbiology.

[20]  C. Jeffries,et al.  RAPID METHOD FOR DETERMINING THE ACTIVITY OF MICROORGAN-ISMS ON NUCLEIC ACIDS , 1957 .

[21]  C. Jeffries,et al.  Rapid method for determining the activity of microorganisms on nucleic acids. , 1957, Journal of bacteriology.