Regulatory effects of miRNA on gastric cancer cells

The present study aimed to screen the regulatory mechanism of microRNA (miR/miRNA) typing on gastric cancer cells in gastric cancer tissues. In total, 46 patients who underwent gastric resection in Xiangya Hospital of Central-South University (Changsha, Hunan, China) between January and December 2012 were selected. Gastric cancer cells were obtained for RNA extraction, and miRNAs were detected by quantitative polymerase chain reaction. Compared with the expression levels in the normal gastric mucosa, the expression levels of miR-9, miR-433, miR-19b and miR-370 were downregulated; the differences was statistically significant, except for miR-19b (P<0.05). miRNAs play a regulative role in the occurrence and development of gastric cancer, and changes in their expression provide a basis for the diagnosis of this disease.

[1]  F. Sato,et al.  Pituitary tumor-transforming 1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma. , 2008, Cancer research.

[2]  Kshitij Srivastava,et al.  Comprehensive Review of Genetic Association Studies and Meta-Analyses on miRNA Polymorphisms and Cancer Risk , 2012, PloS one.

[3]  W. Gerald,et al.  Endogenous human microRNAs that suppress breast cancer metastasis , 2008, Nature.

[4]  X. Qin,et al.  miRNA expression profile in primary gastric cancers and paired lymph node metastases indicates that miR-10a plays a role in metastasis from primary gastric cancer to lymph nodes. , 2012, Experimental and therapeutic medicine.

[5]  J. Martinez-Climent,et al.  Epigenetic regulation of miRNA genes in acute leukemia , 2012, Leukemia.

[6]  Mei Qiang,et al.  Chronic intermittent ethanol exposure and its removal induce a different miRNA expression pattern in primary cortical neuronal cultures. , 2012, Alcoholism, clinical and experimental research.

[7]  W. O'Gorman,et al.  Loop nucleotides control primary and mature miRNA function in target recognition and repression , 2011, RNA biology.

[8]  A. Heimberger,et al.  miRNA-mediated immune regulation and immunotherapeutic potential in glioblastoma. , 2011, Clinical investigation.

[9]  R. Dahiya,et al.  Oncogenic miRNA-182-5p Targets Smad4 and RECK in Human Bladder Cancer , 2012, PloS one.

[10]  H. Budak,et al.  Dehydration stress-responsive miRNA in Brachypodium distachyon: evident by genome-wide screening of microRNAs expression. , 2011, Omics : a journal of integrative biology.

[11]  A. Guimarães,et al.  Increased miRNA-146a and miRNA-155 expressions in oral lichen planus , 2012, Archives of Dermatological Research.

[12]  A. Terzic,et al.  MicroRNA signatures as diagnostic and therapeutic targets. , 2008, Clinical chemistry.

[13]  E. Martin,et al.  Insulin-Like Growth Factor-1 Signaling Regulates miRNA Expression in MCF-7 Breast Cancer Cell Line , 2012, PloS one.

[14]  Ziwei Wang,et al.  [Current status and development of miRNA and siRNA research on gastric cancer]. , 2011, Yi chuan = Hereditas.

[15]  A. Tonevitsky,et al.  Dynamically regulated miRNA-mRNA networks revealed by exercise , 2013, BMC Physiology.

[16]  B. Schermer,et al.  Listeria monocytogenes Infection in Macrophages Induces Vacuolar-Dependent Host miRNA Response , 2011, PloS one.

[17]  Jeyakumar Natarajan,et al.  miRNA-mRNA network detects hub mRNAs and cancer specific miRNAs in lung cancer , 2012, Silico Biol..

[18]  Mohammed Al-Shalalfa,et al.  MicroRNA Response Elements-Mediated miRNA-miRNA Interactions in Prostate Cancer , 2012, Adv. Bioinformatics.

[19]  Min Yan,et al.  MiRNA-199a-3p in Plasma as a Potential Diagnostic Biomarker for Gastric Cancer , 2013, Annals of Surgical Oncology.

[20]  Chun-Hung Lai,et al.  miRNA arm selection and isomiR distribution in gastric cancer , 2012, BMC Genomics.

[21]  O. Kranenburg,et al.  The value of miRNA in diagnosing thyroid cancer: a systematic review. , 2012, Cancer biomarkers : section A of Disease markers.

[22]  R. Gambari,et al.  miRNA therapeutics: delivery and biological activity of peptide nucleic acids targeting miRNAs. , 2011, Epigenomics.

[23]  W. Liu,et al.  A miRNA Binding Site Single-Nucleotide Polymorphism in the 3′-UTR Region of the IL23R Gene Is Associated with Breast Cancer , 2012, PloS one.

[24]  C. Marsit,et al.  Exposure and fetal growth-associated miRNA alterations in the human placenta , 2011, Clinical Epigenetics.

[25]  Haifan Lin,et al.  The miRNA Regulation of Stem Cells. , 2012, Wiley interdisciplinary reviews. Membrane transport and signaling.

[26]  Engineering CHO cell growth by stable manipulation of miRNA expression , 2011, BMC proceedings.

[27]  L. Staudt,et al.  The miRNA-17∼92 cluster mediates chemoresistance and enhances tumor growth in mantle cell lymphoma via PI3K/AKT pathway activation , 2012, Leukemia.

[28]  S. Takeda,et al.  A microRNA regulatory mechanism of osteoblast differentiation , 2009, Proceedings of the National Academy of Sciences.

[29]  Lifu Wang,et al.  Expression and regulatory function of miRNA-34a in targeting survivin in gastric cancer cells , 2013, Tumor Biology.

[30]  Alison A. Staton,et al.  Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo , 2011, Nature Protocols.

[31]  S. Benzina,et al.  MicroRNAs and breast cancer malignancy: an overview of miRNA-regulated cancer processes leading to metastasis. , 2012, Cancer biomarkers : section A of Disease markers.