Mediated amperometric biosensors for lactic acid based on carbon paste electrodes modified with baker's yeast Saccharomyces cerevisiae.

Carbon paste electrodes modified with baker's yeast Saccharomyces cerevisiae (a source of flavocytochrome b(2)) were investigated as amperometric biosensors for lactic acid. Phenazine methosulphate was used as a mediator. The optimal operational conditions of the electrodes were: an operating potential 0.0 V, solution pH 7.2, concentration of phenazine methosulphate in solution 0.2 mM. A linear range in the dependence of the current responses on the concentration of lactic acid was up to 1 mM. The suitability of the electrodes for determination of lactic acid in milk and dairy products such as kefir and yoghurt was tested. The yeast cells in the paste remained viable at least for 1 month.

[1]  Antonio Guerrieri,et al.  Electrosynthesized Bilayer Polymeric Membrane for Effective Elimination of Electroactive Interferents in Amperometric Biosensors , 1995 .

[2]  A. Amine,et al.  Preparation and characterization of a fragile enzyme immobilized carbon paste electrode , 1992 .

[3]  M. Tuite,et al.  Appendix II: Yeast Growth , 1998 .

[4]  Li Jiang,et al.  A reagentless renewable N,N′-diphenyl-p-phenylenediamine loaded sensor for hydrogen sulfide , 2002 .

[5]  Wolfgang Schuhmann,et al.  A novel L-lactate-selective biosensor based on flavocytochrome b2 from methylotrophic yeast Hansenula polymorpha. , 2005, Biosensors & bioelectronics.

[6]  Bansi D. Malhotra,et al.  Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films , 1999 .

[7]  Bansi D. Malhotra,et al.  Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole–polyvinylsulphonate composite films for application to lactate biosensors , 2001 .

[8]  K. Baronian,et al.  The use of yeast and moulds as sensing elements in biosensors. , 2004, Biosensors & bioelectronics.

[9]  Mick F. Tuite,et al.  Yeast gene analysis , 1998 .

[10]  Jean-Michel Kauffmann,et al.  Amperometric biosensor based on carbon paste mixed with enzyme, lipid and cytochrome c , 1994 .

[11]  Klaus-Dieter Vorlop,et al.  Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols , 1995 .

[12]  Željka Filipović-Kovačević,et al.  Cyanide determination in fruit brandies by an amperometric biosensor with immobilised Saccharomyces cerevisiae , 2002 .

[13]  D. Jaros,et al.  Microflora and acidification properties of yogurt and yogurt-related products fermented with commercially available starter cultures. , 1993, International journal of food microbiology.

[14]  Stanislav Miertus,et al.  Hybrid biosensor for the determination of sucrose , 1991 .

[15]  A. Malinauskas,et al.  Investigation of baker's yeast Saccharomyces cerevisiae- and mediator-based carbon paste electrodes as amperometric biosensors for lactic acid , 2003 .

[16]  G. Palleschi,et al.  Enzyme electrode probes obtained by electropolymerization of monomers with PMS and selected dehydrogenase enzymes. , 1997, Talanta.

[17]  J. Kulys,et al.  Reagentless lactate sensor based on cytochrome b2 , 1980 .

[18]  M. Lehmann,et al.  Amperometric measurement of copper ions with a deputy substrate using a novel Saccharomyces cerevisiae sensor. , 2000, Biosensors & bioelectronics.

[19]  J. Kulys,et al.  Alcohol, lactate and glutamate sensors based on oxidoreductases with regeneration of nicotinamide adenine dinucleotide , 1978 .

[20]  N. Pasco,et al.  Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method , 2002, Applied Microbiology and Biotechnology.

[21]  M. Mascini,et al.  Comparison of microbial sensors based on amperometric and potentiometric electrodes , 1986 .

[22]  Mihaela D. Leonida,et al.  Polymeric FAD used as enzyme-friendly mediator in lactate detection , 2003, Analytical and bioanalytical chemistry.

[23]  Hitoshi Yamato,et al.  Design of novel electron transfer mediators based on indophenol derivatives for lactate sensor. , 2002, Biosensors & bioelectronics.

[24]  B. Strehlitz,et al.  Mediator-modified electrodes for electrocatalytic oxidation of NADH , 1995 .

[25]  W. Schuhmann,et al.  Carbon-Paste Electrodes with Incorporated Lactate Oxidase and Mediators , 1992 .

[26]  G. Favero,et al.  Further developments in toxicity cell biosensors , 1997 .

[27]  Lauro T. Kubota,et al.  A Reagentless Amperometric Carbon Paste Based Sensor for NADH , 2000 .

[28]  Lo Gorton,et al.  Prussian Blue- and lactate oxidase-based amperometric biosensor for lactic acid , 2001 .

[29]  C. Bala,et al.  Yeast cells sucrose biosensor based on a potentiometric oxygen electrode , 2002 .

[30]  L. Gorton,et al.  Catalytic oxidation of NADH by surface-modified graphite electrodes , 1981 .

[31]  F. Lederer,et al.  Inactivation of flavocytochrome b2 with fluoropyruvate. Reaction at the active-site histidine. , 1988, European journal of biochemistry.

[32]  Joseph Wang,et al.  Yeast-based carbon paste bioelectrode for ethanol , 1989 .

[33]  M. Koudelka-Hep,et al.  Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators , 2004 .

[34]  I. Willner,et al.  An integrated NAD+-dependent enzyme-functionalized field-effect transistor (ENFET) system: development of a lactate biosensor. , 2000, Biosensors & bioelectronics.

[35]  J. Kulys,et al.  Sensitive yeast bioelectrode to L‐lactate , 1992 .