Distribution and Structural Variation of Nonstarch Polysaccharides in Milling Fractions of Hull-less Barley with Variable Amylose Content

ABSTRACT Three hull-less barley genotypes containing starches with variable amylose content (23.8% normal, 4.3% waxy, 41.8% high-amylose barley) were pearled to 10% and then roller-milled to produce pearling by-products (PBP), flour, and fiber-rich fractions (FRF). PBP were enriched in arabinoxylans, protein, and ash and contained small amounts of starch and β-glucans. FRF were considerably enriched in β-glucans and arabinoxylans. The solubility of β-glucans was higher in PBP than in FRF. The solubility of arabinoxylans was higher in FRF than in PBP. Small amounts of arabinogalactans detected in barley were concentrated in the outer portion of the barley kernel. The content and solubility of nonstarch polysaccharides (NSP) in various milling fractions was also dependent on the type of barley. To obtain more detailed information about the content and molecular structure of NSP, each milling fraction was sequentially extracted with water, alkaline [Ba(OH)2], again with water, and finally with NaOH. These ex...

[1]  J. Kiryluk,et al.  Milling of barley to obtain β‐glucan enriched products , 2000 .

[2]  M. Izydorczyk,et al.  Variation in total and soluble beta-glucan content in hulless barley: effects of thermal, physical, and enzymic treatments. , 2000, Journal of agricultural and food chemistry.

[3]  D. Jacobs,et al.  Consumption of whole-grain foods and decreased risk of cancer : Proposed mechanisms , 2000 .

[4]  A. Klamczynski,et al.  Quality of Flours from Waxy and Nonwaxy Barley for Production of Baked Products , 1999 .

[5]  J. Delcour,et al.  Variation in the degree of D-xylose substitution in water-extractable European durum wheat (Triticum durum desf.) semolina arabinoxylans. , 1999, Journal of agricultural and food chemistry.

[6]  R. S. Bhatty.  β-Glucan and Flour Yield of Hull-less Barley , 1999 .

[7]  C. Biliaderis,et al.  Fractionation of oat (1→3), (1→4)-β-D-glucans and characterisation of the fractions , 1998 .

[8]  M. Izydorczyk,et al.  Structure and physicochemical properties of barley non-starch polysaccharides — I. Water-extractable β-glucans and arabinoxylans☆ , 1998 .

[9]  D. K. Salunkhe,et al.  Barley: chemistry and value-added processing. , 1998, Critical reviews in food science and nutrition.

[10]  R. S. Bhatty.  Milling of Regular and Waxy Starch Hull‐less Barleys for the Production of Bran and Flour , 1997 .

[11]  F. Temelli.  Extraction and Functional Properties of Barley β‐Glucan as Affected by Temperature and pH , 1997 .

[12]  R. Newman,et al.  Influence of the Hulless, Waxy Starch and Short-awn Genes on the Composition of Barleys , 1997 .

[13]  Ho-Moon Seog,et al.  ${\beta}-Glucan$ Enrichment from Pearled Barley and Milled Barley Fractions , 1997 .

[14]  H. Gruppen,et al.  Optimisation of the selective extraction of (Glucurono)arabinoxylans from wheat bran: use of barium and calcium hydroxide solution at elevated temperatures. , 1996 .

[15]  B. Knuckles,et al.  β-glucan enrichment of barley fractions by air classification and sieving , 1995 .

[16]  S. Yoon,et al.  Evaluation of selected barley cultivars and their fractions for β-glucan enrichment and viscosity , 1995 .

[17]  P. Åman,et al.  Fractionation of Different Types of Barley by Roller Milling and Sieving , 1994 .

[18]  B. Blackwell,et al.  Structural studies of (1→3),(1→4)-β-D-glucans by 13C-nuclear magnetic resonance spectroscopy and by rapid analysis of cellulose-like regions using high-performance anion-exchange chromatography of oligosaccharides released by lichenase , 1994 .

[19]  M. Izawa,et al.  Relationship Between Structure and Solubility of (1→3),(1→4)-β-D-Glucan from Barley , 1993 .

[20]  R. S. Bhatty.  Physicochemical properties of roller-milled barley bran and flour , 1993 .

[21]  R. Newman,et al.  Enrichment of tocopherols, tocotrienols, and oil in barley fractions by milling and pearling , 1993 .

[22]  R. S. Bhatty.  β-glucan content and viscosities of barleys and their roller-milled flour and bran products , 1992 .

[23]  R. Newman,et al.  Barley as a food grain , 1991 .

[24]  J. R. Woodward,et al.  Water-soluble (1 → 3,1 → 4)-β-d-glucans from barley (Hordeum vulgare) endosperm. IV. Comparison of 40°C and 65°C soluble fractions , 1988 .

[25]  D. Brant,et al.  The sequence statistics and solution conformation of a barley (1→3, 1→)-β-d-glucan , 1986 .

[26]  R. Henry.  Genetic and environmental variation in the pentosan and β-glucan contents of barley, and their relation to malting quality , 1986 .

[27]  M. Glennie-Holmes,et al.  ENZYMIC QUANTIFICATION OF (1→3) (1→4)‐β‐D‐GLUCAN IN BARLEY AND MALT , 1985 .

[28]  H. Englyst,et al.  Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates , 1984 .

[29]  R. Marchessault,et al.  Crystalline conformation and structure of lichenan and barley β-glucan , 1983 .

[30]  J. R. Woodward,et al.  Water-soluble (1→3), (1→4)-β-D-glucans from barley (Hordeum vulgare) endosperm. II. Fine structure , 1983 .

[31]  S. Douglas.  A rapid method for the determination of pentosans in wheat flour , 1981 .

[32]  A. Bacic,et al.  Isolation and Ultrastructure of Aleurone Cell Walls From Wheat and Barley , 1981 .

[33]  G. Fincher.  Morphology and chemical composition of barley endosperm cell walls , 1975 .

[34]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.