Determination of Uric Acid in Human Saliva and Urine Using Miniaturized Capillary Electrophoresis with Amperometric Detection

An improved method based on a novel miniaturized capillary electrophoresis with amperometric detection (CE-AD) system was described to study the correlation between the trace amount of uric acid (UA) in human saliva and urine samples for patients suffering from gout disease. Under the optimum conditions, UA can been well separated from its co-existing potential interferences, namely xanthine, hypoxanthine, and ascorbic acid, within 170 s with estimated separation efficiency of 111,210 TP m−1. The proposed miniaturized CE-AD system has been successfully applied for the analysis of UA in less invasive biological samples, which could provide potential application in fast diagnosis of gout.

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