Quantitative CARS molecular fingerprinting of single living cells with the use of the maximum entropy method.

This work was supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support from the Precursory Research for Embryonic Science and Technology (PRESTO) program of JST. The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. F. Omura and H. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample. We gratefully acknowledge J. Ukon (HORIBA, Ltd.) for assisting in the collaboration between the Japanese and French groups.

[1]  Yasuaki Naito,et al.  In vivo time‐resolved Raman imaging of a spontaneous death process of a single budding yeast cell , 2005 .

[2]  Andreas Volkmer,et al.  Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles , 2002 .

[3]  Stephen R. Leone,et al.  Chemical Imaging of Photoresists with Coherent Anti-Stokes Raman Scattering (CARS) Microscopy , 2004 .

[4]  M D Duncan,et al.  Scanning coherent anti-Stokes Raman microscope. , 1982, Optics letters.

[5]  J. Greve,et al.  Vibrational bands of luminescent zinc(II)-octaethylporphyrin using a polarization-sensitive microscopic multiplex CARS technique , 2001 .

[6]  Mortazavi,et al.  Supporting Online Material Materials and Methods Figs. S1 to S13 Tables S1 to S3 References Label-free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy , 2022 .

[7]  Juleon M. Schins,et al.  Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy , 2002 .

[8]  Hiro-o Hamaguchi,et al.  Raman spectroscopic signature of life in a living yeast cell , 2004 .

[9]  Mischa Bonn,et al.  Direct extraction of Raman line-shapes from congested CARS spectra. , 2006, Optics express.

[10]  Katrin F. Domke,et al.  Label-free chemical imaging of catalytic solids by coherent anti-Stokes Raman scattering and synchrotron-based infrared microscopy. , 2009, Angewandte Chemie.

[11]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[12]  Hiro-o Hamaguchi,et al.  Broadband (1000 cm−1) multiplex CARS spectroscopy: Application to polarization sensitive and time-resolved measurements , 1994 .

[13]  J. Greve,et al.  Studying single living cells and chromosomes by confocal Raman microspectroscopy , 1990, Nature.

[14]  S. Kawata,et al.  Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration. , 2000, Optics letters.

[15]  Hiro-o Hamaguchi,et al.  Molecular-level investigation of the structure, transformation, and bioactivity of single living fission yeast cells by time- and space-resolved Raman spectroscopy. , 2005, Biochemistry.

[16]  Dirk Roos,et al.  Intracellular Chemical Imaging of Heme-Containing Enzymes Involved in Innate Immunity Using Resonance Raman Microscopy , 2004 .

[17]  Vladislav V. Yakovlev,et al.  Comparison of coherent and spontaneous Raman microspectroscopies for noninvasive detection of single bacterial endospores , 2007, Proceedings of the National Academy of Sciences.

[18]  Hiro-o Hamaguchi,et al.  Supercontinuum dynamically visualizes a dividing single cell. , 2007, Analytical chemistry.

[19]  Yaron Silberberg,et al.  Single-pulse coherently controlled nonlinear Raman spectroscopy and microscopy , 2002, Nature.