Quick detection technique for clenbuterol hydrochloride by using surface plasmon resonance biosensor

Abstract Clenbuterol hydrochloride (CLB) is a kind of lean meat powder which can increase the conversion rate of lean meat. Though it is allowed in human and veterinary medicine, CLB is harmful for human body and, therefore, is restricted to its use as a growth promoter. In this paper, a quick detection technique for clenbuterol hydrochloride is proposed. The technique, which is based on surface plasmon resonance (SPR) and immune reaction, includes two methods, the direct method and the suppression method. The direct method can be applied, to screen an antibody and in the study of the dynamic reaction. For the immune suppression method, the concentration of CLB is inversely proportional to the change of SPR angle. The detection limit of this method is smaller than 2 μg/L, and the pork extraction is detected. The concentration of CLB in the sample is 2.75 μg/L, and the concentration of antibody is 250 μg/L. The detection of CLB by SPR is quick, highly sensitive, and label free. And the utilized equipment is cheap, simple, environmental friendly, and easy to control. The proposed technique could achieve the real-time detection of a large number of samples and be widely used to detect CLB in all fields of food processing. Therefore, this method is expected to be used in the inspection process for foods in the supermarket, bazaar, and factory.

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