Biomarkers of oxidative stress in Wallago attu (Bl. and Sch.) during and after a fish-kill episode at Panipat, India.

The present investigation was carried out by sampling water, sediment and fish during a fish-kill episode at Panipat (Haryana, India), and again sampling at the same site was conducted after a gap of two months. During the second sampling no fish-kill was observed and the water was relatively less turbid and clear. Antioxidant profile and lipid peroxidation (LPO) in fish tissues were studied. Analysis was also carried out on the physico-chemical characteristics of water samples along with heavy metal and pesticide analysis in water and sediment samples during and after the episode. Dissolved oxygen level was substantially low during the fish-kill episode. Heavy metals (copper and chromium) and pesticides like BHC (Benzene hexachloride), DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) were also detected during the episode in water and sediment samples. Various oxidative stress biomarkers in liver, kidney and gill tissues in the Indian freshwater fish Wallago attu (Bl. and Sch.) collected from the site were investigated. The levels of reduced glutathione and non-protein thiol were significantly (P<0.001) higher in the liver of Wallago attu collected from Panipat after the fish-kill episode. Ascorbic acid levels in all the tissues did not change significantly after the episode. The LPO in liver, kidney and gills was significantly low (P<0.01-0.001) in all tissues of fish collected after the fish-kill episode. The protein carbonyl levels were significantly low (P<0.05-0.01) in all the fish organs sampled after the fish-kill episode. The findings suggest that industrial effluent may result in the massive loss of a commercial commodity. The simultaneous measurement of the physicochemical parameters of the water samples showed a good correlation between the biomarkers responses and the environmental chemical stress conditions.

[1]  R. Huggett,et al.  Biomarkers: Biochemical, Physiological, and Histological Markers of Anthropogenic Stress , 1992 .

[2]  Richard T. Di Giulio,et al.  Prooxidant and antioxidant mechanisms in aquatic organisms , 1991 .

[3]  E. Stadtman,et al.  Metal-catalyzed oxidation of proteins. Physiological consequences. , 1991, The Journal of biological chemistry.

[4]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[5]  K. Oakes,et al.  Oxidative stress responses in longnose sucker (Catostomus catostomus) exposed to pulp and paper mill and municipal sewage effluents. , 2004, Aquatic toxicology.

[6]  I. Sayeed,et al.  Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallago attu (Bl. & Schn.). , 2003, The Science of the total environment.

[7]  A. Meister.  1 – ON THE BIOCHEMISTRY OF GLUTATHIONE , 1989 .

[8]  R. Evans,et al.  Toxicology of dietary nickel in lake whitefish (Coregonus clupeaformis). , 2002, Aquatic toxicology.

[9]  K. Oakes,et al.  Utility of the TBARS assay in detecting oxidative stress in white sucker (Catostomus commersoni) populations exposed to pulp mill effluent. , 2003, Aquatic toxicology.

[10]  D. W. Filho,et al.  Seasonal changes in antioxidant defenses of the digestive gland of the brown mussel (Perna perna) , 2001 .

[11]  S. Raisuddin,et al.  Effects of Paraquat on the Freshwater Fish Channa punctata (Bloch): Non-Enzymatic Antioxidants as Biomarkers of Exposure , 2006, Archives of environmental contamination and toxicology.

[12]  I. Sayeed,et al.  Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.) , 2007, Biological Trace Element Research.

[13]  J. Sturve,et al.  Oxidative damage in eelpout (Zoarces viviparus), measured as protein carbonyls and TBARS, as biomarkers. , 2005, Aquatic toxicology.

[14]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[15]  S. Raisuddin,et al.  Copper modulates non-enzymatic antioxidants in the freshwater fish Channa punctata (Bloch) exposed to deltamethrin. , 2006, Chemosphere.

[16]  Abdul Malik,et al.  Genotoxicity of the Yamuna River water at Okhla (Delhi), India. , 2005, Ecotoxicology and environmental safety.

[17]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[18]  D. Livingstone.  Biotechnology and pollution monitoring: Use of molecular biomarkers in the aquatic environment , 1993 .

[19]  B. Hasspieler,et al.  Glutathione-dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). , 1994, Ecotoxicology and environmental safety.

[20]  K. Güven,et al.  Acute Lethal Toxicity and Accumulation of Copper in Gammarus pulex (L.) (Amphipoda) , 1999 .

[21]  M. Pacheco,et al.  Biotransformation, endocrine, and genetic responses of Anguilla anguilla L. to petroleum distillate products and environmentally contaminated waters. , 2001, Ecotoxicology and environmental safety.

[22]  J. Albaigés,et al.  Effects of the “Aegean Sea” oil spill on biotransformation enzymes, oxidative stress and DNA-adducts in digestive gland of the mussel (Mytilus edulus L.) , 1996 .

[23]  R. Wenning,et al.  Biochemical responses in aquatic animals: A review of determinants of oxidative stress , 1989 .

[24]  A. Matos,et al.  Assessment of Water Systems for Contaminants from Domestic and Industrial Sewages , 2002, Environmental monitoring and assessment.

[25]  P. Thomas,et al.  Effects of metals and organic compounds on hepatic glutathione, cysteine, and acid-soluble thiol levels in mullet (Mugil cephalus L.). , 1984, Toxicology and applied pharmacology.

[26]  C. Rojas,et al.  Simultaneous determination of two antioxidants, uric and ascorbic acid, in animal tissue by high-performance liquid chromatography. , 1991, Analytical biochemistry.

[27]  Thomas F. Waters,et al.  Sediment in streams: Sources, biological effects, and control , 1995 .

[28]  T. Gabryelak,et al.  The effect of paraquat on the peroxide metabolism enzymes in erythrocytes of freshwater fish species. , 1985, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[29]  D. W. Filho.  Fish antioxidant defenses--a comparative approach. , 1996 .

[30]  J. Sedlák,et al.  Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. , 1968, Analytical biochemistry.

[31]  T. S. Gill,et al.  Gill, liver, and kidney lesions associated with experimental exposures to carbaryl and dimethoate in the fish (Puntius conchonius ham.) , 1988, Bulletin of Environmental Contamination and Toxicology.

[32]  S. Raisuddin,et al.  Protein carbonyls: novel biomarkers of exposure to oxidative stress-inducing pesticides in freshwater fish Channa punctata (Bloch). , 2005, Environmental toxicology and pharmacology.

[33]  D. Ribera,et al.  Responses of antioxidants and lipid peroxidation in mussels to oxidative damage exposure. , 1991, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[34]  A. Castaño,et al.  Toxicity identification evaluations for the investigation of fish kills: a case study. , 1994, Chemosphere.

[35]  Jonny Beyer,et al.  Fish bioaccumulation and biomarkers in environmental risk assessment: a review. , 2003, Environmental toxicology and pharmacology.

[36]  C. Sarasquete,et al.  Histological changes and copper accumulation in liver and gills of the Senegales sole, Solea senegalensis. , 1999, Ecotoxicology and environmental safety.

[37]  E. Gallagher,et al.  Effects of Black Rock Harbor sediments on indices of biotransformation, oxidative stress, and DNA integrity in channel catfish , 1993 .

[38]  E. Stadtman,et al.  Determination of carbonyl content in oxidatively modified proteins. , 1990, Methods in enzymology.

[39]  John S. Gray,et al.  Background and rationale to a practical workshop on biological effects of pollutants , 1988 .

[40]  M. Uchiyama,et al.  Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. , 1978, Analytical biochemistry.

[41]  D. Jollow,et al.  Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. , 1974, Pharmacology.

[42]  S. Jain,et al.  Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. , 2000, Biochimica et biophysica acta.