Molecular correlates of influenza A H5N1 virus pathogenesis in mice.

Highly pathogenic avian influenza A H5N1 viruses caused an outbreak of human respiratory illness in Hong Kong. Of 15 human H5N1 isolates characterized, nine displayed a high-, five a low-, and one an intermediate-pathogenicity phenotype in the BALB/c mouse model. Sequence analysis determined that five specific amino acids in four proteins correlated with pathogenicity in mice. Alone or in combination, these specific residues are the likely determinants of virulence of human H5N1 influenza viruses in this model.

[1]  S. Schultz-Cherry,et al.  Influenza virus neuraminidase activates latent transforming growth factor beta , 1996, Journal of virology.

[2]  Malbea A Lapete,et al.  Prevention and Control of Influenza Recommendations of the Advisory Committee on Immunization Practices ( ACIP ) , 2004 .

[3]  A. Ward,et al.  Neurovirulence of influenza A virus , 1996 .

[4]  N. Cox,et al.  Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. , 1998, Science.

[5]  S. Schultz-Cherry,et al.  Distinct Pathogenesis of Hong Kong-Origin H5N1 Viruses in Mice Compared to That of Other Highly Pathogenic H5 Avian Influenza Viruses , 2000, Journal of Virology.

[6]  H. Goto,et al.  A novel mechanism for the acquisition of virulence by a human influenza A virus. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. Peiris,et al.  Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus , 1998, The Lancet.

[8]  B. Murphy,et al.  A single amino acid in the PB2 gene of influenza A virus is a determinant of host range , 1993, Journal of virology.

[9]  Tokiko Watanabe,et al.  Generation of influenza A viruses entirely from cloned cDNAs. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[10]  R. Webster,et al.  A pandemic warning? , 1997, Nature.

[11]  J. Almond.  A single gene determines the host range of influenza virus , 1977, Nature.

[12]  N. Cox,et al.  Characterization of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997-1998. , 1999, Virology.

[13]  C. Scholtissek,et al.  Correlation of pathogenicity and gene constellation of influenza A viruses. II. Highly neurovirulent recombinants derived from non-neurovirulent or weakly neurovirulent parent virus strains. , 1979, Virology.

[14]  B. Murphy,et al.  Rescue of an influenza A virus wild-type PB2 gene and a mutant derivative bearing a site-specific temperature-sensitive and attenuating mutation , 1993, Journal of virology.

[15]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[16]  R. Webster,et al.  Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. , 1996, Avian diseases.

[17]  P. Palese,et al.  Glycosylation of neuraminidase determines the neurovirulence of influenza A/WSN/33 virus , 1993, Journal of virology.

[18]  W. J. Bean,et al.  Characterization of virulent and avirulent A/chicken/Pennsylvania/83 influenza A viruses: potential role of defective interfering RNAs in nature , 1985, Journal of virology.

[19]  R. Webster,et al.  Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus , 1998, The Lancet.

[20]  E. Brown,et al.  Genetic analysis of mouse-adapted influenza A virus identifies roles for the NA, PB1, and PB2 genes in virulence. , 1999, Virus research.

[21]  M. Vey,et al.  Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin‐like endoprotease. , 1992, The EMBO journal.

[22]  J. N. Varghese,et al.  Structure of the catalytic and antigenic sites in influenza virus neuraminidase , 1983, Nature.

[23]  A. García-Sastre,et al.  Rescue of influenza A virus from recombinant DNA. , 2007, Journal of virology.

[24]  S. Lindstrom,et al.  Characterization of low virulent strains of highly pathogenic A/Hong Kong/156/97 (H5N1) virus in mice after passage in embryonated hens' eggs. , 2000, Virology.

[25]  B. Murphy,et al.  Four viral genes independently contribute to attenuation of live influenza A/Ann Arbor/6/60 (H2N2) cold-adapted reassortant virus vaccines , 1988, Journal of virology.

[26]  K. Omoe,et al.  Evolutionary characterization of the six internal genes of H5N1 human influenza A virus. , 2000, The Journal of general virology.

[27]  C. Sweet,et al.  Role of neuraminidase in influenza virus-induced apoptosis. , 1999, The Journal of general virology.

[28]  H. Goto,et al.  Biological Heterogeneity, Including Systemic Replication in Mice, of H5N1 Influenza A Virus Isolates from Humans in Hong Kong , 1999, Journal of Virology.

[29]  N. Cox,et al.  A Mouse Model for the Evaluation of Pathogenesis and Immunity to Influenza A (H5N1) Viruses Isolated from Humans , 1999, Journal of Virology.

[30]  T. Tumpey,et al.  Depletion of Lymphocytes and Diminished Cytokine Production in Mice Infected with a Highly Virulent Influenza A (H5N1) Virus Isolated from Humans , 2000, Journal of Virology.

[31]  David E. Swayne,et al.  Comparisons of Highly Virulent H5N1 Influenza A Viruses Isolated from Humans and Chickens from Hong Kong , 1998, Journal of Virology.

[32]  R. Webster,et al.  Characterization of influenza A/HongKong/156/97 (H5N1) virus in a mouse model and protective effect of zanamivir on H5N1 infection in mice. , 1998, The Journal of infectious diseases.

[33]  Y. Guan,et al.  Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. , 1998, Virology.