Phosphate Uptake by Cyanobacteria Is Associated with Kinetic Fractionation of Phosphate Oxygen Isotopes

The oxygen isotope ratio within phosphate (δ18OP) is an emerging powerful tool capable of providing valuable information on phosphorus (P) biogeochemistry. Microorganisms play a central role in phosphorus cycling in the environment, and microbial activity may result in both equilibrium and kinetic fractionation of phosphate oxygen isotopes. In the present paper, we focus on kinetic fractionation induced by microbial phosphorus uptake. This type of fractionation may result in nonequilibrium δ18OP values observed in some marine, freshwater, and terrestrial settings. We worked with the model cyanobacterium, Synechocystis PCC6803, which has two pst (phosphate specific transporter) type phosphate (PO4) transporters, each characterized by a different affinity for PO4. We found that PO4 uptake by Synechocystis in the P-uptake experiments induced fractionation when cells were P-limited. Moreover, under these conditions, Synechocystis preferentially took up the isotopically lighter P16O4. This resulted in a PO4–wa...

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