Redistribution of intracellular Ca2+ in mitogen-stimulated human peripheral blood lymphocytes.

The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in CTC fluorescence indicating the release of membrane-bound Ca2+. The effect was independent of the level of extracellular Ca2+ and could be observed in the presence of EGTA; it was suppressed by the metabolic inhibitors FCCP, antimycin and sodium cyanide. Analysis of the excitation spectra of CTC fluorescence indicated that the observed effect is caused by redistribution of intracellular Ca2+ rather than Mg2+. Thus the lectin interaction with the lymphocyte plasma membrane results in Ca2+ release into the cytosol from the intracellular stores.

[1]  Y. Oron,et al.  Concanavalin A-stimulated Ca2+ uptake in rat splenocytes , 1980, Molecular and Cellular Biochemistry.

[2]  M. Sikorska,et al.  The regulation of cell proliferation by calcium and cyclic AMP , 1979, Molecular and Cellular Biochemistry.

[3]  M. Brand,et al.  The isolation of lymphocyte mitochondria and their regulation of extramitochondrial free Ca2+ concentration. , 1982, Biochemical Journal.

[4]  R. Tsien.  A non-disruptive technique for loading calcium buffers and indicators into cells , 1981, Nature.

[5]  M. Crumpton,et al.  Extracellular Ca2+ requirement for initiation of lymphocyte growth. , 1980, Biochemical Society transactions.

[6]  R. Mikkelsen,et al.  Concanavalin A induces the release of intracellular Ca2+ in intact rabbit thymocytes. , 1980, The Journal of biological chemistry.

[7]  M. B. Feinstein,et al.  Release of intracellular membrane-bound calcium precedes the onset of stimulus-induced exocytosis in platelets. , 1980, Biochemical and biophysical research communications.

[8]  P. Naccache,et al.  Chemotactic factor-induced release of membrane calcium in rabbit neutrophils. , 1979, Science.

[9]  H. Rasmussen,et al.  The messenger function of calcium in cell activation. , 1979, Symposia of the Society for Experimental Biology.

[10]  F. Bygrave.  MITOCHONDRIA AND THE CONTROL OF INTRACELLULAR CALCIUM , 1978, Biological reviews of the Cambridge Philosophical Society.

[11]  D. Chandler,et al.  Intracellular divalent cation release in pancreatic acinar cells during stimulus-secretion coupling. II. Subcellular localization of the fluorescent probe chlorotetracycline , 1978, The Journal of cell biology.

[12]  D. Chandler,et al.  Intracellular divalent cation release in pancreatic acinar cells during stimulus-secretion coupling. I. Use of chlorotetracycline as fluorescent probe , 1978, The Journal of cell biology.

[13]  M. Houslay,et al.  Is an early calcium flux necessary to stimulate lymphocytes? , 1977, Nature.

[14]  E. J. Moticka,et al.  A SINGLE SECOND SIGNAL FOR MITOGENESIS , 1977 .

[15]  M. Crompton,et al.  The Sodium‐Induced Efflux of Calcium from Heart Mitochondria , 1976 .

[16]  S. K. Holian,et al.  A potassium ionophore (valinomycin) inhibits lymphocyte proliferation by its effects on the cell membrane. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Luthra,et al.  Studies of mitochondrial calcium movements using chlorotetracycline. , 1976, Biochimica et biophysica acta.

[18]  L. J. Roth,et al.  Direct evidence for intracellular divalent cation redistribution associated with platelet shape change. , 1976, Biochemical and biophysical research communications.

[19]  D. Babcock,et al.  Action of ionophore A23187 at the cellular level. Separation of effects at the plasma and mitochondrial membranes. , 1976, The Journal of biological chemistry.

[20]  R. Jilka,et al.  Effect of the purified (Mg2+ + Ca2+)-activated ATPase of sarcoplasmic reticulum upon the passive Ca2+ permeability and ultrastructure of phospholipid vesicles. , 1975, The Journal of biological chemistry.

[21]  A. Ulmer,et al.  The control of immune response in vitro by Ca2+. II. The Ca2+-dependent period during mitogenic stimulation. , 1975, Immunology.

[22]  A. Caswell,et al.  Observation of calcium uptake by isolated sarcoplasmic reticulum employing a fluorescent chelate probe. , 1972, Biochemical and biophysical research communications.

[23]  A. Caswell,et al.  Visualization of membrane bound cations by a fluorescent technique. , 1971, Biochemical and biophysical research communications.

[24]  A. Böyum.  Isolation of leucocytes from human blood. Further observations. Methylcellulose, dextran, and ficoll as erythrocyteaggregating agents. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[25]  H. D. du Buy,et al.  Selective Localization of Tetracycline in Mitochondria of Living Cells , 1961, Science.