Changes in the expression of Fas, osteonectin and osteocalcin with age in the rabbit growth plate.

Chondrocytes of the growth plate are generally assumed to undergo apoptosis, but the mechanisms which induce this cell death are not known. The Fas receptor is a mediator of the apoptotic signal in some systems. We studied its expression in situ in growth plates of rabbits aged from five to 20 weeks. In addition, we investigated the immunolocalisation in the growth plates of the bone proteins, osteonectin and osteocalcin, and the changes in their expression with age. The Fas-positive chondrocytes were found mostly in the hypertrophic zone, as were the osteonectin-positive and osteocalcin-positive cells. The percentage of Fas-positive cells increased with age whereas little change was found in the number of osteonectin-positive and osteocalcin-positive chondrocytes. Many of the Fas-positive chondrocytes were also TUNEL-positive. This strongly suggests that apoptosis in the growth plate is mediated through the Fas system. Double immunostaining for osteocalcin and Fas showed that not all hypertrophic chondrocytes were of the same cell type. Some chondrocytes stained for osteocalcin only, others for Fas only, while some were positive for both.

[1]  S. Kokubun,et al.  Apoptosis and proliferation of growth plate chondrocytes in rabbits. , 1997, The Journal of bone and joint surgery. British volume.

[2]  Y. Sasaguri,et al.  MORPHOLOGICAL AND BIOCHEMICAL EVIDENCE FOR APOPTOSIS IN THE TERMINAL HYPERTROPHIC CHONDROCYTES OF THE GROWTH PLATE , 1996, The Journal of pathology.

[3]  S. Nagata,et al.  Apoptosis: Telling cells their time is up , 1996, Current Biology.

[4]  E. Burger,et al.  DNA fragmentation during bone formation in neonatal rodents assessed by transferase‐mediated end labeling , 1996, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[5]  L. Gerstenfeld,et al.  Expression of bone‐specific genes by hypertrophic chondrocytes: Implications of the complex functions of the hypertrophic chondrocyte during endochondral bone development , 1996, Journal of cellular biochemistry.

[6]  I. Shapiro,et al.  End labeling studies of fragmented DNA in the Avian growth plate: Evidence of apoptosis in terminally differentiated chondrocytes , 1995, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[7]  T. Aigner,et al.  Osteogenic differentiation of hypertrophic chondrocytes involves asymmetric cell divisions and apoptosis , 1995, The Journal of cell biology.

[8]  M. Schaffler,et al.  Chondrocyte apoptosis in endochondral ossification of chick sterna , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[9]  A. Krasinskas,et al.  Shared phenotypic expression of osteoblasts and chondrocytes in fracture callus , 1995, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[10]  S. Yonehara,et al.  Involvement of apoptosis antigen Fas in clonal deletion of human thymocytes. , 1994, International immunology.

[11]  J. Reeve,et al.  A number of osteocalcin antisera recognize epitopes on proteins other than osteocalcin in cultured skin fibroblasts: Implications for the identification of cells of the osteoblastic lineage in vitro , 1994, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[12]  H. Roach,et al.  WHY DOES BONE MATRIX CONTAIN NON‐COLLAGENOUS PROTEINS? THE POSSIBLE ROLES OF OSTEOCALCIN, OSTEONECTIN, OSTEOPONTIN AND BONE SIALOPROTEIN IN BONE MINERALISATION AND RESORPTION , 1994, Cell biology international.

[13]  Roach Hi.  Why Does Bone Matrix Contain Non-Collagenous Proteins? The Possible Roles of Osteocalcin, Osteonectin, Osteopontin and Bone Sialoprotein in Bone Mineralisation and Resorption , 1994 .

[14]  O. Oni,et al.  Localisation of bone‐forming cells during fracture healing by osteocalcin immunocytochemistry: An experimental study of the rabbit tibia , 1994, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[15]  S. Nagata,et al.  Lethal effect of the anti-Fas antibody in mice , 1993, Nature.

[16]  S. Hakomori,et al.  Ley antigen expression is correlated with apoptosis (programmed cell death) , 1993 .

[17]  J. Lian,et al.  Induction of bone‐related proteins, osteocalcin and osteopontin, and their matrix ultrastructural localization with development of chondrocyte hypertrophy in vitro , 1993, Journal of cellular biochemistry.

[18]  M. Glimcher,et al.  High‐resolution immunolocalization of osteopontin and osteocalcin in bone and cartilage during endochondral ossification in the chicken tibia , 1992, The Anatomical record.

[19]  M. Silbermann,et al.  Chondroclasts and endothelial cells collaborate in the process of cartilage resorption , 1992, The Anatomical record.

[20]  F. Hino,et al.  A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies. , 1991, Journal of immunological methods.

[21]  I. Shapiro,et al.  Hypertrophic Chondrocytes , 1990, Annals of the New York Academy of Sciences.

[22]  W. Robertson.  Newest knowledge of the growth plate. , 1990, Clinical orthopaedics and related research.

[23]  E. Closs,et al.  Gene expression during osteogenic differentiation in mandibular condyles in vitro , 1990, The Journal of cell biology.

[24]  C. Farnum,et al.  Cellular turnover at the chondro‐osseous junction of growth plate cartilage: Analysis by serial sections at the light microscopical level , 1989, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[25]  S. Yonehara,et al.  A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor , 1989, The Journal of experimental medicine.

[26]  K. Aozasa,et al.  Prognostic significance of Ki‐67 reactivity in soft tissue sarcomas , 1989, Cancer.

[27]  K.,et al.  Quantitation of chondrocyte performance in growth-plate cartilage during longitudinal bone growth. , 1987, The Journal of bone and joint surgery. American volume.

[28]  H Stein,et al.  Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[29]  M. Sporn,et al.  Autocrine secretion and malignant transformation of cells. , 1980, The New England journal of medicine.

[30]  M. Silbermann,et al.  Heterogeneity among chondrocytes of the mandibular condyle in foetal and postnatal mice. , 1973, Archives of oral biology.

[31]  B. Stanger.  Looking beneath the Surface: The Cell Death Pathway of Fas/APO-1 (CD95) , 1996, Molecular medicine.

[32]  B. Tillmann,et al.  [Structure and function of the growth plate]. , 1996, Zeitschrift fur Orthopadie und ihre Grenzgebiete.

[33]  G. Evan,et al.  Apoptosis and the cell cycle. , 1995, Current opinion in cell biology.

[34]  M. R. Simon,et al.  Cellular heterogeneity and insulin-like growth factor I immunoreactivity among epiphysial growth plate chondrocytes in the pig. , 1988, Acta anatomica.

[35]  J. Lian,et al.  Osteocalcin. Biochemical considerations and clinical applications. , 1988, Clinical orthopaedics and related research.

[36]  P. Hauschka,et al.  Light and electron microscopic demonstration of osteocalcin antigenicity in embryonic and adult rat bone. , 1986, Bone.

[37]  PHILIP,et al.  The Journal of Bone and Joint Surgery. , 1950, Annals of the Royal College of Surgeons of England.