A photoelectrochemical method for tracking the motion of Daphnia magna in water.

We present the novel use of photoelectrochemistry to detect and monitor the motion of a single Daphnia magna swimming in a confined volume of water. Using an array of individually-addressable electrodes under illumination and potentiostatted so that a photocurrent is generated, the motion of the daphnid is detected by means of measuring "dark" transients as the shadow cast by the moving sphere passes over each electrode. The method can be used to determine the size and speed of a single daphnid.

[1]  S. Dodson,et al.  Size-specific swimming behavior of Daphnia pulex , 1991 .

[2]  C. Amatore,et al.  Synthesis and electrochemical properties of fullerene-rich nanoclusters synthesized by cobalt-catalyzed cyclotrimerization of bis(aryl)alkyne fullerodendrimers. , 2007, Angewandte Chemie.

[3]  Joseph Wang,et al.  Carbon-nanotube-induced acceleration of catalytic nanomotors. , 2008, ACS nano.

[4]  C. Ogino,et al.  Fractal analysis of Daphnia motion for acute toxicity bioassay , 2002, Environmental toxicology.

[5]  F. Harnisch,et al.  Detection of the adhesion events of dispersed single montmorillonite particles at a static mercury drop electrode , 2004 .

[6]  Frank Schweitzer,et al.  Modeling Vortex Swarming In Daphnia , 2004, Bulletin of mathematical biology.

[7]  T. Lauridsen,et al.  A comparison of feeding efficiency and swimming ability of Daphnia magna exposed to cypermethrin. , 2005, Aquatic toxicology.

[8]  Junho Jeon,et al.  Development of a new biomonitoring method to detect the abnormal activity of Daphnia magna using automated Grid Counter device. , 2008, The Science of the total environment.

[9]  Richard G Compton,et al.  A method for the positioning and tracking of small moving particles. , 2009, Angewandte Chemie.

[10]  A. Weber,et al.  Swimming behaviour of Daphnia clones: differentiation through predator infochemicals , 2002 .

[11]  S. Ameta,et al.  Use of the thionine-EDTA system in photogalvanic cells for solar energy conversion , 1989 .

[12]  Daniel Bratton,et al.  The electrochemical detection of droplets in microfluidic devices. , 2008, Lab on a chip.

[13]  P. Moore,et al.  Studying Daphnia feeding behavior as a black box: a novel electrochemical approach , 2007, Hydrobiologia.

[14]  C. Banks,et al.  Sonoelectrochemistry Understood via Nanosecond Voltammetry: Sono-emulsions and the Measurement of the Potential of Zero Charge of a Solid Electrode , 2002 .

[15]  Richard G Compton,et al.  Ultrafast chronoamperometry of single impact events in acoustically agitated solid particulate suspensions. , 2006, Chemphyschem : a European journal of chemical physics and physical chemistry.

[16]  Michael L. Johns,et al.  Chronoamperometric Sizing and Locating of Spherical Particles via Potential-Step Transients , 2007 .

[17]  Richard G Compton,et al.  Voltammetric sizing of particles: chronoamperometry of impact events in acoustically agitated particulate suspensions. , 2007, The Analyst.

[18]  R. Compton,et al.  A photoelectrochemical method for determining the kinematics of moving particles using an array of individually addressable electrodes. , 2009, Chemistry, an Asian journal.

[19]  R. Compton,et al.  Photoelectrochemical ESR: Part I. Experimental , 1983 .

[20]  T. Jones,et al.  Voltammetric sizing of a sphere. , 2006, Chemphyschem : a European journal of chemical physics and physical chemistry.

[21]  R. Blust,et al.  Analysis of the swimming velocity of cadmium-stressed Daphnia magna , 1999 .