A regenerable immunochip for the rapid determination of 13 different antibiotics in raw milk.

Access to high-quality and safe food is a basic need in our community and, consequently, the European Union has defined maximum residue levels (MRLs) for a number of antibacterial compounds. However, despite the obvious demand for quantitative multi-residue detection methods that can be carried out on a routine basis, there is currently a lack in the development of such systems. In particular, an automated multianalyte detection instrument is needed that is capable of quantifying several antibiotics simultaneously within minutes. The newly developed hapten microarrays are designed for the parallel analysis of 13 different antibiotics in milk within six minutes by applying an indirect competitive chemiluminescence microarray immunoassay (CL-MIA). To allow multiple analyses, a regenerable microarray chip was developed based on epoxy-activated PEG chip surfaces, onto which microspotted antibiotic derivatives like sulfonamides, beta-lactams, aminoglycosides, fluorquinolones and polyketides are coupled directly without further use of linking agents. Using the chip reader platform MCR 3, this antigen solid phase is stable for at least 50 consecutive analyses.

[1]  R. Hintsche,et al.  Automated detection and quantitation of bacterial RNA by using electrical microarrays. , 2006, Analytical chemistry.

[2]  S. Kır,et al.  Improved determination of quinolones in milk at their MRL levels using LC-UV, LC-FD, LC-MS and LC-MS/MS and validation in line with regulation 2002/657/EC. , 2008, Analytica chimica acta.

[3]  Reinhard Niessner,et al.  Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in water using a flow-through chemiluminescence microarray readout system. , 2008, Analytical chemistry.

[4]  Reinhard Niessner,et al.  Automated analytical microarrays: a critical review , 2008, Analytical and bioanalytical chemistry.

[5]  Reinhard Niessner,et al.  Highly parallel affinity sensor for the detection of environmental contaminants in water , 1999 .

[6]  Janos Vörös,et al.  Optical microarray biosensing techniques , 2006 .

[7]  .. G.H.R.JahedKhaniki,et al.  Chemical Contaminants in Milk and Public Health Concerns: A Review , 2007 .

[8]  M. Sharman,et al.  Detection of streptomycin and dihydrostreptomycin residues in milk, honey and meat samples using an optical biosensor. , 2002, The Analyst.

[9]  Gert Ludwig Duveneck,et al.  Planar waveguides for ultra-high sensitivity of the analysis of nucleic acids , 2002 .

[10]  R. Dietrich,et al.  Immunochemical detection of antibiotics and sulfonamides. , 1994, The Analyst.

[11]  R. Dietrich,et al.  Immunochemical rapid test for multiresidue analysis of antimicrobial drugs in milk using monoclonal antibodies and hapten–glucose oxidase conjugates , 2003 .

[12]  S. Nicolardi,et al.  A simple and rapid assay based on hot water extraction and liquid chromatography–tandem mass spectrometry for monitoring quinolone residues in bovine milk , 2008 .

[13]  R. Dietrich,et al.  The potential of monoclonal antibodies against ampicillin for the preparation of a multi-immunoaffinity chromatography for penicillins. , 1998, The Analyst.

[14]  Reinhard Niessner,et al.  Development of an open stand-alone platform for regenerable automated microarrays. , 2009, Biosensors & bioelectronics.

[15]  Yves Babin,et al.  A high-throughput analytical method for determination of aminoglycosides in veal tissues by liquid chromatography/tandem mass spectrometry with automated cleanup. , 2007, Journal of AOAC International.

[16]  Reinhard Niessner,et al.  Preparation and characterization of functional poly(ethylene glycol) surfaces for the use of antibody microarrays. , 2007, Analytical chemistry.

[17]  Ana M García-Campaña,et al.  Multiresidue method for the determination of quinolone antibiotics in bovine raw milk by capillary electrophoresis-tandem mass spectrometry. , 2006, Analytical chemistry.

[18]  J. Nouws,et al.  A microbiological assay system for assessment of raw milk exceeding EU maximum residue levels , 1999 .

[19]  Yolanda Picó,et al.  Progress in analysis of residual antibacterials in food , 2007 .

[20]  Elisa Michelini,et al.  Peer Reviewed: Analytical Bioluminescence and Chemiluminescence , 2003 .

[21]  A. van Amerongen,et al.  Application of an immunosensor for the detection of the β-lactam antibiotic, cephalexin , 2003 .

[22]  Reinhard Niessner,et al.  Automated microarray system for the simultaneous detection of antibiotics in milk. , 2004, Analytical chemistry.

[23]  E. Benito-Peña,et al.  Development of a novel and automated fluorescent immunoassay for the analysis of beta-lactam antibiotics. , 2005, Journal of agricultural and food chemistry.

[24]  Lisa C Shriver-Lake,et al.  The Array Biosensor: Portable, Automated Systems , 2007, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[25]  R. Stadler,et al.  Simultaneous Determination of Five β-Lactam Antibiotics in Bovine Milk Using Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry , 2001 .

[26]  Christophe A. Marquette,et al.  Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies. , 2008, Biosensors & bioelectronics.

[27]  Guenter Gauglitz,et al.  Automated water analyser computer supported system (AWACSS) Part I: Project objectives, basic technology, immunoassay development, software design and networking. , 2005, Biosensors & bioelectronics.

[28]  Michael Petz,et al.  Development of a receptor-based microplate assay for the detection of beta-lactam antibiotics in different food matrices. , 2007, Analytica chimica acta.