Human trichinellosis in Sourthern Spain: serologic and epidemiologic study.

An outbreak of trichinellosis caused by wild boar meat occurred in the Iruela (Jaen) in southern Spain in February 1996. Thirty-five people were diagnosed on the basis of epidemiologic data, but only 24 patients agreed to participate in this study. Twenty-three (96%) had symptoms suggestive of trichinellosis. Immunofluorescent and Western blot test results for trichinellosis were positive in 18 persons, and 15 had circulating Trichinella spiralis antigens. These findings suggest that results of tests for circulating antigens in conjunction with clinical presentation are useful for the diagnosis of trichinellosis.

[1]  E. Pozio Trichinellosis in the European union: epidemiology, ecology and economic impact. , 1998, Parasitology today.

[2]  D. Despommier,et al.  Clinical aspects of infection with Trichinella spp , 1996, Clinical microbiology reviews.

[3]  T. Garate,et al.  Use of random amplified polymorphic DNA for detection of Trichinella britovi outbreaks in Spain. , 1995, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[4]  G. Tiberio,et al.  Short report: an outbreak of trichinosis in Navarra, Spain. , 1995, The American journal of tropical medicine and hygiene.

[5]  C. Bandi,et al.  Identification of Trichinella pseudospiralis from a human case using random amplified polymorphic DNA. , 1995, The American journal of tropical medicine and hygiene.

[6]  M. McNeil,et al.  Characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae. , 1993, Molecular and biochemical parasitology.

[7]  E. Denkers,et al.  Trichinella spiralis: influence of an immunodominant, carbohydrate-associated determinant on the host antibody response repertoire. , 1991, Experimental parasitology.

[8]  M. Gómez-Morales,et al.  Trichinellosis in southern Spain. , 1990, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[9]  M. Gómez-Morales,et al.  Seroepidemiological studies on five outbreaks of trichinellosis in southern Spain. , 1990, Annals of tropical medicine and parasitology.

[10]  H. Gamble,et al.  Comparative efficacy of antigen and antibody detection tests for human trichinellosis. , 1989, The Journal of parasitology.

[11]  J. Dupouy-Camet,et al.  Different aetiological agents cause the European outbreaks of horsemeat induced human trichinellosis. , 1989 .

[12]  H. Gamble Trichinella spiralis: immunization of mice using monoclonal antibody affinity-isolated antigens. , 1985, Experimental parasitology.

[13]  R. Parkhouse,et al.  Characterization of surface antigens of Trichinella spiralis infective larvae , 1981, Parasite immunology.

[14]  R. Parkhouse,et al.  Changing proteins on the surface of a parasitic nematode , 1980, Nature.

[15]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[16]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[17]  M. Steinbuch,et al.  The isolation of IgG from mammalian sera with the aid of caprylic acid. , 1969, Archives of biochemistry and biophysics.

[18]  A. Sulzer Indirect fluorescent antibody tests for parasitic diseases. I. Preparation of a stable antigen from larvae of Trichinella spiralis. , 1965, The Journal of parasitology.

[19]  J. Williams,et al.  Fluorescent antibody test for the serological diagnosis of trichinosis. , 1962, Experimental parasitology.