3D Printed Scaffolds as a New Perspective for Bone Tissue Regeneration: Literature Review

Due to the high incidence of bone fractures in the population, it became necessary to produce scaffolds that are able to assist in tissue regeneration. It is necessary to find an appropriate balance between the mechanical and biological properties, in order to mimic the natural tissue, these properties are directly related to the architecture and their degree of porosity, as well as the size of their pores and their interconnectivity. In this perspective, the 3D printing stands out, where the structure is obtained layer by layer, according to a predetermined computational model which provides a greater control of architecture and scaffold geometry and overcomes, in this way, the limitations of traditional techniques of scaffolds manufacturing. In this way, the objective of this seminar is to present the state of the art of the polymer scaffolds produced by 3D printing and applied to bone tissue regeneration, highlighting the advantages and limitations of this process.

[1]  M. Vallet‐Regí,et al.  Calcium phosphates as substitution of bone tissues , 2004 .

[2]  D. Therriault,et al.  Solvent-cast three-dimensional printing of multifunctional microsystems. , 2013, Small.

[3]  Anthony Atala,et al.  Printing Technologies for Medical Applications. , 2016, Trends in molecular medicine.

[4]  J T Czernuszka,et al.  Novel 3D collagen scaffolds fabricated by indirect printing technique for tissue engineering. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[5]  Barry Berman,et al.  3D printing: the new industrial revolution , 2012, IEEE Engineering Management Review.

[6]  A. Boccaccini,et al.  Tissue Engineering Scaffolds from Bioactive Glass and Composite Materials , 2008 .

[7]  Darrin J. Pochan,et al.  Polymer Nanocomposites for Biomedical Applications , 2007 .

[8]  Hae-Won Kim,et al.  Naturally and synthetic smart composite biomaterials for tissue regeneration. , 2013, Advanced drug delivery reviews.

[9]  Brian Derby,et al.  Selective cell delivery for 3D tissue culture and engineering , 2004 .

[10]  Kah Fai Leong,et al.  Cryogenic prototyping of chitosan scaffolds with controlled micro and macro architecture and their effect on in vivo neo-vascularization and cellular infiltration. , 2010, Journal of biomedical materials research. Part A.

[11]  D. Sarkar,et al.  Preparation of porous scaffold from hydroxyapatite powders , 2011 .

[12]  M Navarro,et al.  Biomaterials in orthopaedics , 2008, Journal of The Royal Society Interface.

[13]  Christian Bergmann,et al.  3D printing of bone substitute implants using calcium phosphate and bioactive glasses , 2010 .

[14]  A. Atala,et al.  Carbon nanotube applications for tissue engineering. , 2007, Biomaterials.

[15]  Mikaël M. Martino,et al.  Biomimetic materials in tissue engineering , 2010 .

[16]  M. Fathi,et al.  Novel hydroxyapatite–forsterite–bioglass nanocomposite coatings with improved mechanical properties , 2011 .

[17]  A. Boccaccini,et al.  Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[18]  Jorge Rodrigues,et al.  Structural evaluation of scaffolds prototypes produced by three-dimensional printing , 2011 .

[19]  R. Reis,et al.  Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments , 2004 .

[20]  Maria Farsari,et al.  Direct laser writing , 2015 .

[21]  T. Ono,et al.  The mechanisms of UV mutagenesis. , 2011, Journal of radiation research.

[22]  Dong-Woo Cho,et al.  A new method of fabricating robust freeform 3D ceramic scaffolds for bone tissue regeneration , 2013, Biotechnology and bioengineering.

[23]  Xing‐dong Zhang,et al.  Osteoinduction by calcium phosphate biomaterials , 1998, Journal of materials science. Materials in medicine.

[24]  Nicholas Dunne,et al.  Printability of calcium phosphate: calcium sulfate powders for the application of tissue engineered bone scaffolds using the 3D printing technique. , 2014, Materials science & engineering. C, Materials for biological applications.

[25]  Dong-Woo Cho,et al.  Evaluating cell proliferation based on internal pore size and 3D scaffold architecture fabricated using solid freeform fabrication technology , 2010, Journal of materials science. Materials in medicine.

[26]  Dong-Woo Cho,et al.  Three-dimensional printing of rhBMP-2-loaded scaffolds with long-term delivery for enhanced bone regeneration in a rabbit diaphyseal defect. , 2014, Tissue engineering. Part A.

[27]  R. Detsch,et al.  Fabrication of Tailored Hydroxyapatite Scaffolds: Comparison between a Direct and an Indirect Rapid Prototyping Technique , 2007 .

[28]  S. Milz,et al.  Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing , 2005, Journal of materials science. Materials in medicine.

[29]  G. Arealis,et al.  Bone printing: new frontiers in the treatment of bone defects. , 2015, Injury.

[30]  M. Fernández-Gutiérrez,et al.  Bio-safe fabrication of PLA scaffolds for bone tissue engineering by combining phase separation, porogen leaching and scCO2 drying , 2015 .

[31]  L. Griffith,et al.  Tissue Engineering--Current Challenges and Expanding Opportunities , 2002, Science.

[32]  D. Cho,et al.  3D printing of cell-laden constructs for heterogeneous tissue regeneration , 2013 .

[33]  S. Van Vlierberghe,et al.  Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review. , 2011, Biomacromolecules.

[34]  Weimin Li,et al.  3D scaffolds in breast cancer research. , 2016, Biomaterials.

[35]  Jonathan C Knowles,et al.  Hydroxyapatite/poly(epsilon-caprolactone) composite coatings on hydroxyapatite porous bone scaffold for drug delivery. , 2004, Biomaterials.

[36]  F. Klocke,et al.  Consolidation phenomena in laser and powder-bed based layered manufacturing , 2007 .

[37]  Benjamin M Wu,et al.  Recent advances in 3D printing of biomaterials , 2015, Journal of Biological Engineering.

[38]  A. Bandyopadhyay,et al.  Bone tissue engineering using 3D printing , 2013 .

[39]  Xuedong Zhou,et al.  Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells , 2014, Bone Research.

[40]  Jean-Pierre Kruth,et al.  Composites by rapid prototyping technology , 2010 .

[41]  J. Fisher,et al.  Soft and hard tissue response to photocrosslinked poly(propylene fumarate) scaffolds in a rabbit model. , 2002, Journal of biomedical materials research.

[42]  A. Sannino,et al.  Influence of the calcination temperature on morphological and mechanical properties of highly porous hydroxyapatite scaffolds , 2013 .

[43]  Peter X Ma,et al.  Biomimetic polymer/apatite composite scaffolds for mineralized tissue engineering. , 2004, Macromolecular bioscience.

[44]  I. Rajzer,et al.  Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application , 2014, Journal of Materials Science.

[45]  G. Kiebzak.  Age-related bone changes , 1991, Experimental Gerontology.

[46]  Hyoun‐Ee Kim,et al.  In vitro/in vivo biocompatibility and mechanical properties of bioactive glass nanofiber and poly(epsilon-caprolactone) composite materials. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[47]  Joshua M. Pearce,et al.  Polymer recycling codes for distributed manufacturing with 3-D printers , 2015 .

[48]  C. Yatongchai,et al.  Influence of preparation method on hydroxyapatite porous scaffolds , 2011 .

[49]  Ling Li,et al.  Prospective study of iliac crest bone graft harvest site pain and morbidity. , 2009, The spine journal : official journal of the North American Spine Society.

[50]  Suprakas Sinha Ray,et al.  POLYMER/LAYERED SILICATE NANOCOMPOSITES: A REVIEW FROM PREPARATION TO PROCESSING , 2003 .

[51]  Jonathan Goole,et al.  3D printing in pharmaceutics: A new tool for designing customized drug delivery systems. , 2016, International journal of pharmaceutics.

[52]  William Bonfield,et al.  Designing porous scaffolds for tissue engineering , 2006, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[53]  S J Hollister,et al.  Manufacturing and Characterization of 3‐D Hydroxyapatite Bone Tissue Engineering Scaffolds , 2002, Annals of the New York Academy of Sciences.

[54]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[55]  L. Flynn,et al.  The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells. , 2010, Biomaterials.

[56]  M. S. Joshi,et al.  Effect of inclusion size on mechanical properties of polymeric composites with micro and nano particles , 2006 .

[57]  Shoufeng Yang,et al.  Rapid prototyping of ceramic lattices for hard tissue scaffolds , 2008 .

[58]  Malcolm N. Cooke,et al.  Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[59]  H. Gleiter,et al.  Nanostructured materials: basic concepts and microstructure☆ , 2000 .

[60]  Peter van Gelderen,et al.  Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells , 2001, Nature Biotechnology.

[61]  Yang Wei-we,et al.  A Review on , 2008 .

[62]  Richard O.C. Oreffo,et al.  Biofabrication of bone tissue: approaches, challenges and translation for bone regeneration. , 2016, Biomaterials.

[63]  D. Cho,et al.  Biomimetic 3D tissue printing for soft tissue regeneration. , 2015, Biomaterials.

[64]  Uwe Gbureck,et al.  3D powder printed calcium phosphate implants for reconstruction of cranial and maxillofacial defects. , 2010, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery.

[65]  K. Leong,et al.  Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. , 2003, Biomaterials.

[66]  Xue Yan,et al.  PII: 0010-4485(95)00035-6 , 2003 .

[67]  Yen Wah Tong,et al.  Characterization of porous poly(D,L‐lactic‐co‐glycolic acid) sponges fabricated by supercritical CO2 gas‐foaming method as a scaffold for three‐dimensional growth of Hep3B cells , 2008, Biotechnology and bioengineering.

[68]  Bin Duan,et al.  Customized Ca–P/PHBV nanocomposite scaffolds for bone tissue engineering: design, fabrication, surface modification and sustained release of growth factor , 2010, Journal of The Royal Society Interface.

[69]  B. Pingguan-Murphy,et al.  Fabrication and characterization of elastomeric scaffolds comprised of a citric acid-based polyester/hydroxyapatite microcomposite , 2013 .

[70]  M. Prabhakaran,et al.  Biomimetic hydroxyapatite-containing composite nanofibrous substrates for bone tissue engineering , 2010, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[71]  R. L. Walton,et al.  Progress in adipose tissue construct development. , 2003, Clinics in plastic surgery.

[72]  H. H. Malik,et al.  Three-dimensional printing in surgery: a review of current surgical applications. , 2015, The Journal of surgical research.

[73]  E. Fortunati,et al.  Multifunctional nanostructured PLA materials for packaging and tissue engineering , 2013 .

[74]  Marinella Levi,et al.  Conductive 3D microstructures by direct 3D printing of polymer/carbon nanotube nanocomposites via liquid deposition modeling , 2015 .

[75]  Daniel Roy Eyers,et al.  Assessment of non-uniform shrinkage in the laser sintering of polymer materials , 2013 .

[76]  Amir Arifin,et al.  Material processing of hydroxyapatite and titanium alloy (HA/Ti) composite as implant materials using powder metallurgy: A review , 2014 .

[77]  Rocky S Tuan,et al.  Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture. , 2013, Biomaterials.

[78]  T. Webster,et al.  Nanotechnology and nanomaterials: Promises for improved tissue regeneration , 2009 .

[79]  J. Rosen,et al.  Modelling breast cancer: one size does not fit all , 2007, Nature Reviews Cancer.

[80]  J. Planell,et al.  High-resolution PLA-based composite scaffolds via 3-D printing technology. , 2013, Acta biomaterialia.

[81]  H. Uludaǧ,et al.  Growth Factor Delivery for Bone Tissue Engineering , 2001, Journal of drug targeting.

[82]  Joaquim Ciurana,et al.  Selecting Process Parameters in RepRap Additive Manufacturing System for PLA Scaffolds Manufacture , 2013 .

[83]  Ralph Müller,et al.  Printability of calcium phosphate powders for three-dimensional printing of tissue engineering scaffolds. , 2012, Acta biomaterialia.

[84]  Judith M Curran,et al.  The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate. , 2006, Biomaterials.

[85]  Youxin Hu,et al.  Mechanical characteristics of solid-freeform-fabricated porous calcium polyphosphate structures with oriented stacked layers. , 2011, Acta biomaterialia.

[86]  F. Melchels,et al.  A review on stereolithography and its applications in biomedical engineering. , 2010, Biomaterials.

[87]  James J. Yoo,et al.  Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology. , 2013, Biomaterials.

[88]  C K Chua,et al.  Development of tissue scaffolds using selective laser sintering of polyvinyl alcohol/hydroxyapatite biocomposite for craniofacial and joint defects , 2004, Journal of materials science. Materials in medicine.

[89]  John D Currey,et al.  Tensile yield in compact bone is determined by strain, post-yield behaviour by mineral content. , 2004, Journal of biomechanics.

[90]  K. Khalil,et al.  Preparation and In Vitro Thermo-Mechanical Characterization of Electrospun PLGA Nanofibers for Soft and Hard Tissue Replacement , 2013, International Journal of Electrochemical Science.

[91]  Paulo Jorge Da Silva bartolo,et al.  Characterisation of PCL and PCL/PLA Scaffolds for Tissue Engineering☆ , 2013 .

[92]  Jan Feijen,et al.  A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomaterials.

[93]  Xiaomin Zhu,et al.  The crystallization behavior of porous poly(lactic acid) prepared by modified solvent casting/particulate leaching technique for potential use of tissue engineering scaffold , 2014, 1404.3930.

[94]  Benjamin M. Wu,et al.  Scaffold fabrication by indirect three-dimensional printing. , 2005, Biomaterials.

[95]  Peter X Ma,et al.  Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. , 2003, Journal of biomedical materials research. Part A.

[96]  Abdolreza Simchi,et al.  Fabrication of a highly ordered hierarchically designed porous nanocomposite via indirect 3D printing: Mechanical properties and in vitro cell responses , 2015 .

[97]  I G Turner,et al.  Fabrication of HA/TCP scaffolds with a graded and porous structure using a camphene-based freeze-casting method. , 2009, Acta biomaterialia.

[98]  D. Hutmacher,et al.  Scaffold development using 3D printing with a starch-based polymer , 2002 .

[99]  C A van Blitterswijk,et al.  3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties. , 2006, Biomaterials.

[100]  P. Bártolo,et al.  Additive manufacturing of tissues and organs , 2012 .

[101]  Aldo R. Boccaccini,et al.  Preparation and characterisation of poly(lactide-co-glycolide) (PLGA) and PLGA/Bioglass® composite tubular foam scaffolds for tissue engineering applications , 2005 .

[102]  Thomas Scanlon,et al.  The development of a three-dimensional imaging system and its application in computer aided design workstations , 2003 .

[103]  M Bohner,et al.  Structural and material approaches to bone tissue engineering in powder-based three-dimensional printing. , 2011, Acta biomaterialia.

[104]  Peter Fratzl,et al.  The effect of geometry on three-dimensional tissue growth , 2008, Journal of The Royal Society Interface.

[105]  Chong Wang,et al.  Electrospun multifunctional tissue engineering scaffolds , 2014, Frontiers of Materials Science.

[106]  D. W. Pack,et al.  Microspheres for Drug Delivery , 2006, BioMEMS and Biomedical Nanotechnology.

[107]  Cheng Yan,et al.  Polyurethane (PU) scaffolds prepared by solvent casting/particulate leaching (SCPL) combined with centrifugation , 2010 .

[108]  Peter Dubruel,et al.  A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. , 2012, Biomaterials.

[109]  M. L. Ferreira,et al.  PLA nano- and microparticles for drug delivery: an overview of the methods of preparation. , 2007, Macromolecular bioscience.

[110]  Seung-Schik Yoo,et al.  Generation of Multi-scale Vascular Network System Within 3D Hydrogel Using 3D Bio-printing Technology , 2014, Cellular and molecular bioengineering.

[111]  Eleftherios Tsiridis,et al.  Bone substitutes: an update. , 2005, Injury.

[112]  Jan H. Kietzmann,et al.  Disruptions, decisions, and destinations: enter the age of 3-D printing and additive manufacturing , 2015, IEEE Engineering Management Review.

[113]  Po-Chun Chang,et al.  Bone tissue engineering with novel rhBMP2-PLLA composite scaffolds. , 2007, Journal of biomedical materials research. Part A.

[114]  Emanuel M. Sachs,et al.  Solid free-form fabrication of drug delivery devices , 1996 .

[115]  Margam Chandrasekaran,et al.  Rapid prototyping in tissue engineering: challenges and potential. , 2004, Trends in biotechnology.

[116]  Theo H. Smit,et al.  Biodegradable Polymers in Bone Tissue Engineering , 2009, Materials.

[117]  Huipin Yuan,et al.  BIOMATERIALS : CURRENT KNOWLEDGE OF PROPERTIES , EXPERIMENTAL MODELS AND BIOLOGICAL MECHANISMS , 2011 .

[118]  K. Anselme,et al.  Osteoblast adhesion on biomaterials. , 2000, Biomaterials.

[119]  J. Dunlop,et al.  Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells , 2010, Biointerphases.

[120]  P. Giannoudis,et al.  Tissue regeneration. The past, the present and the future. , 2005, Injury.

[121]  Inmaculada Plaza,et al.  Challenges, issues and trends in fall detection systems , 2013, Biomedical engineering online.

[122]  Sie Chin Tjong,et al.  STRUCTURAL AND MECHANICAL PROPERTIES OF POLYMER NANOCOMPOSITES , 2006 .

[123]  O. Lame,et al.  Microstructural origin of physical and mechanical properties of polyamide 12 processed by laser sintering , 2012 .

[124]  Robert Langer,et al.  The History of Tissue Engineering Using Synthetic Biodegradable Polymer Scaffolds and Cells , 1997 .

[125]  L G Griffith,et al.  Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. , 2001, Tissue engineering.

[126]  Michael F. Ashby,et al.  The mechanical efficiency of natural materials , 2004 .

[127]  Wei Sun,et al.  Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro. , 2007, Biomaterials.

[128]  P H Krebsbach,et al.  Indirect solid free form fabrication of local and global porous, biomimetic and composite 3D polymer-ceramic scaffolds. , 2003, Biomaterials.

[129]  Seeram Ramakrishna,et al.  Development of nanocomposites for bone grafting , 2005 .

[130]  Florencia Edith Wiria,et al.  Property enhancement of 3D-printed alumina ceramics using vacuum infiltration , 2014 .

[131]  Eduardo Saiz,et al.  Direct ink writing of highly porous and strong glass scaffolds for load-bearing bone defects repair and regeneration. , 2011, Acta biomaterialia.

[132]  L G Griffith,et al.  Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels. , 1998, Annals of surgery.

[133]  Krishnendu Roy,et al.  Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[134]  Wonhye Lee,et al.  Bio-printing of collagen and VEGF-releasing fibrin gel scaffolds for neural stem cell culture , 2010, Experimental Neurology.

[135]  Jorge Vicente Lopes da Silva,et al.  Organ Printing as an Information Technology , 2015 .

[136]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[137]  Frank A. Müller,et al.  Direct Printing of Bioceramic Implants with Spatially Localized Angiogenic Factors , 2007 .

[138]  T. Park,et al.  Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation. , 1999, Journal of biomedical materials research.

[139]  Neil Hopkinson,et al.  Rapid manufacturing : an industrial revolution for the digital age , 2006 .

[140]  Larry L Hench,et al.  Third-Generation Biomedical Materials , 2002, Science.

[141]  H. Ohgushi,et al.  BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: topology of osteogenesis. , 1998, Journal of biomedical materials research.

[142]  G. Naughton,et al.  Emerging developments in tissue engineering and cell technology. , 1995, Tissue engineering.

[143]  Jayanta Debnath,et al.  Modelling glandular epithelial cancers in three-dimensional cultures , 2005, Nature Reviews Cancer.

[144]  V. Zhdanov,et al.  Enhancement of protein adsorption induced by surface roughness. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[145]  Lorenzo Moroni,et al.  Biomaterials engineered for integration , 2008 .

[146]  Cato T. Laurencin,et al.  Nanostructured Scaffolds for Bone Tissue Engineering , 2011 .

[147]  Stephen F Badylak,et al.  Decellularization of tissues and organs. , 2006, Biomaterials.

[148]  Inês Pires,et al.  Design and production of sintered β-tricalcium phosphate 3D scaffolds for bone tissue regeneration , 2012 .

[149]  T. Park,et al.  A novel fabrication method of macroporous biodegradable polymer scaffolds using gas foaming salt as a porogen additive. , 2000, Journal of biomedical materials research.

[150]  Jeffrey W Stansbury,et al.  3D printing with polymers: Challenges among expanding options and opportunities. , 2016, Dental materials : official publication of the Academy of Dental Materials.

[151]  Qing Li,et al.  On stiffness of scaffolds for bone tissue engineering-a numerical study. , 2010, Journal of biomechanics.

[152]  D. Odde,et al.  Laser-guided direct writing for applications in biotechnology. , 1999, Trends in biotechnology.

[153]  Besim Ben-Nissan,et al.  Natural bioceramics: from coral to bone and beyond , 2003 .

[154]  F. Senatov,et al.  Mechanical properties and shape memory effect of 3D-printed PLA-based porous scaffolds. , 2016, Journal of the mechanical behavior of biomedical materials.

[155]  Eduardo Napadensky,et al.  Bioprinting and Tissue Engineering: Recent Advances and Future Perspectives , 2013 .

[156]  M. Ueda,et al.  Injectable soft-tissue augmentation by tissue engineering and regenerative medicine with human mesenchymal stromal cells, platelet-rich plasma and hyaluronic acid scaffolds. , 2009, Cytotherapy.

[157]  E H Burger,et al.  Mineralization processes in demineralized bone matrix grafts in human maxillary sinus floor elevations. , 1999, Journal of biomedical materials research.

[158]  C. Mothersill,et al.  Induction of c-myc oncoprotein and of cellular proliferation by radiation in normal human urothelial cultures. , 1991, Anticancer research.

[159]  J. Vacanti,et al.  Tissue engineering: a 21st century solution to surgical reconstruction. , 2001, The Annals of thoracic surgery.

[160]  R. Watson,et al.  A low-cost surgical application of additive fabrication. , 2014, Journal of surgical education.

[161]  Samantha J. Paulsen,et al.  Tissue vascularization through 3D printing: Will technology bring us flow? , 2015, Developmental dynamics : an official publication of the American Association of Anatomists.

[162]  Kriskrai Sitthiseripratip,et al.  3D printing of hydroxyapatite: Effect of binder concentration in pre-coated particle on part strength , 2007 .

[163]  Shuping Peng,et al.  Structure and properties of nano-hydroxypatite scaffolds for bone tissue engineering with a selective laser sintering system , 2011, Nanotechnology.

[164]  C. Chua,et al.  Effects of layer thickness and binder saturation level parameters on 3D printing process , 2011 .

[165]  T. Boland,et al.  Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. , 2003, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology.

[166]  Xiaofeng Cui,et al.  Application of inkjet printing to tissue engineering , 2006, Biotechnology journal.

[167]  Mehran Solati-Hashjin,et al.  Effect of technical parameters on porous structure and strength of 3D printed calcium sulfate prototypes , 2016 .

[168]  K. Leong,et al.  Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends. , 2003, Biomaterials.

[169]  Sophie C Cox,et al.  3D printing of porous hydroxyapatite scaffolds intended for use in bone tissue engineering applications. , 2015, Materials science & engineering. C, Materials for biological applications.

[170]  I. Zein,et al.  Fused deposition modeling of novel scaffold architectures for tissue engineering applications. , 2002, Biomaterials.

[171]  S. Nazhat,et al.  Polylactic acid (PLA) biomedical foams for tissue engineering , 2014 .

[172]  Yusuf Khan,et al.  Bone graft substitutes , 2006, Expert review of medical devices.

[173]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[174]  Anton J.M. Schoot Uiterkamp,et al.  A global sustainability perspective on 3D printing technologies , 2014 .

[175]  MironovVladimir,et al.  Direct laser writing , 2015 .

[176]  A. Elaissari,et al.  Nanotechnology olymer-based nanocapsules for drug delivery , 2009 .

[177]  Lorenz Meinel,et al.  Localized delivery of growth factors for bone repair. , 2004, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[178]  Dichen Li,et al.  The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing. , 2015, Materials science & engineering. C, Materials for biological applications.

[179]  Abhay S Pandit,et al.  Porous titanium scaffolds fabricated using a rapid prototyping and powder metallurgy technique. , 2008, Biomaterials.

[180]  E. Saiz,et al.  Direct write assembly of calcium phosphate scaffolds using a water-based hydrogel. , 2010, Acta biomaterialia.

[181]  Jennifer S. Park,et al.  The effect of matrix stiffness on the differentiation of mesenchymal stem cells in response to TGF-β. , 2011, Biomaterials.

[182]  G. Mestres,et al.  Role of pore size and morphology in musculo-skeletal tissue regeneration. , 2016, Materials science & engineering. C, Materials for biological applications.

[183]  I. Morita,et al.  Biocompatible inkjet printing technique for designed seeding of individual living cells. , 2005, Tissue engineering.

[184]  R. Narayan,et al.  Laser direct writing of micro- and nano-scale medical devices , 2010, Expert review of medical devices.

[185]  Rui L Reis,et al.  Bone tissue engineering: state of the art and future trends. , 2004, Macromolecular bioscience.

[186]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[187]  I. Zuburtikudis,et al.  Porous poly(l-lactic acid) nanocomposite scaffolds prepared by phase inversion using supercritical CO2 as antisolvent , 2007 .

[188]  M. Mozafari,et al.  Development of macroporous nanocomposite scaffolds of gelatin/bioactive glass prepared through layer solvent casting combined with lamination technique for bone tissue engineering , 2010 .

[189]  Eva Syková,et al.  Nanotechnologies in regenerative medicine , 2010, Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy.

[190]  D. Brunette,et al.  Effects of substratum surface topography on the organization of cells and collagen fibers in collagen gel cultures. , 2002, Journal of biomedical materials research.

[191]  Arjan Vissink,et al.  Resorbable screws for fixation of autologous bone grafts. , 2006, Clinical oral implants research.

[192]  Hyoun‐Ee Kim,et al.  Highly porous hydroxyapatite bioceramics with interconnected pore channels using camphene-based freeze casting , 2007 .

[193]  Judit Tulla-Puche,et al.  Polymers and drug delivery systems. , 2012, Current drug delivery.

[194]  K. Lu,et al.  Effect of particle size on three dimensional printed mesh structures , 2009 .

[195]  P. Uppanan,et al.  PLA-HA Scaffolds: Preparation and Bioactivity☆ , 2013 .

[196]  K. Popat,et al.  Bone tissue engineering: A review in bone biomimetics and drug delivery strategies , 2009, Biotechnology progress.

[197]  Ali Khademhosseini,et al.  Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography. , 2012, Biomaterials.

[198]  F. Petzoldt,et al.  Effect of production parameters on the properties of IN 718 superalloy by three-dimensional printing , 2008 .

[199]  Tabatabaei Qomi,et al.  The Design of Scaffolds for Use in Tissue Engineering , 2014 .

[200]  Anthony Atala,et al.  3D bioprinting of tissues and organs , 2014, Nature Biotechnology.

[201]  Y. Ohya,et al.  Encapsulation and/or release behavior of bovine serum albumin within and from polylactide-grafted dextran microspheres. , 2004, Macromolecular bioscience.

[202]  A. Bandyopadhyay,et al.  Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[203]  P. E. Casillas,et al.  Preparation of hydroxyapatite nanoparticles facilitated by the presence of β-cyclodextrin , 2012 .