In vivo SPECTROPHOTOMETRIC EVALUATION OF NEOPLASTIC AND NON‐NEOPLASTIC SKIN PIGMENTED LESIONS–I. REFLECTANCE MEASUREMENTS

Abstract— Reflectance spectrophotometry from 400 to 800 nm on different cutaneous pigmented lesions, including primary and metastatic malignant melanoma, pigmented nevi, lentigo and seborrhoeic keratosis, has been performed by using an external integrating sphere coupled to a spectrophotometer. Measurements show that reflectance spectra of the different lesions manifest dissimilar patterns, particularly in the near IR region. Comparison of reflectance of nevi with that of malignant melanomas results in a highly significant difference (P < 10‐6) between the two samples. Though interpretation of the specta remains difficult as a result of the complexity of the optical processes of scattering and absorption, our results suggest that a detailed analysis of the reflectance spectrum may give clinically useful information, and could be utilized as an aid in clinical diagnosis of cutaneous pigmented lesions, especially where malignant melanoma is concerned.

[1]  D. G. Goebel Generalized integrating-sphere theory. , 1967, Applied optics.

[2]  N Kollias,et al.  Absorption mechanisms of human melanin in the visible, 400-720 nm. , 1987, The Journal of investigative dermatology.

[3]  F. Grum 5 – Radiation Sources , 1979 .

[4]  A. Srivastava,et al.  The significance of blood flow in cutaneous malignant melanoma demonstrated by Doppler flowmetry. , 1986, European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology.

[5]  N Kollias,et al.  ON THE ASSESSMENT OF MELANIN IN HUMAN SKIN in vivo *, † . , 1986, Photochemistry and photobiology.

[6]  S D Walter,et al.  Observer perception of skin color in a study of malignant melanoma. , 1987, American journal of epidemiology.

[7]  D. J. Ellis,et al.  A theoretical and experimental study of light absorption and scattering by in vivo skin. , 1980, Physics in medicine and biology.

[8]  James C. Daly Fiber Optics , 1984 .

[9]  J. Feather,et al.  A portable scanning reflectance spectrophotometer using visible wavelengths for the rapid measurement of skin pigments. , 1989, Physics in medicine and biology.

[10]  M L Wolbarsht,et al.  Melanin, a unique biological absorber. , 1981, Applied optics.

[11]  S L Jacques,et al.  IMMEDIATE PIGMENT DARKENING: VISUAL AND REFLECTANCE SPECTROPHOTOMETRIC ANALYSIS OF ACTION SPECTRUM , 1990, Photochemistry and photobiology.

[12]  B. Wilson,et al.  Monte Carlo modeling of light propagation in highly scattering tissues. I. Model predictions and comparison with diffusion theory , 1989, IEEE Transactions on Biomedical Engineering.

[13]  S. Jacques,et al.  Angular dependence of HeNe laser light scattering by human dermis , 1988 .

[14]  N Kollias,et al.  Spectroscopic characteristics of human melanin in vivo. , 1985, The Journal of investigative dermatology.

[15]  K. Jimbow,et al.  Fine structural characterization of melanosomes in dysplastic nevi , 1985, Cancer.

[16]  D. J. Ellis,et al.  A portable reflectometer for the rapid quantification of cutaneous haemoglobin and melanin. , 1988, Physics in medicine and biology.