Gamma-tubulin and MTOCs in Paramecium.

The characterization of the two Paramecium gamma-tubulin genes, gammaPT1 and gammaPT2, allowed us to raise Paramecium-specific antibodies, directed against their most divergent carboxy-terminal peptide and to analyze the localization and dynamics of gamma-tubulin throughout the cell cycle. As in other cell types, a large proportion of the protein was found to be cytosolic, but in contrast to the general situation, gamma-tubulin was found to be permanently associated to four types of sites: basal bodies, the micronuclear compartment--within which mitotic and meiotic spindles develop without membrane breakdown, the pores of the contractile vacuoles and the cytoproct which are cortical microtubular organelles fulfilling excretory functions. In addition, a transient site of gamma-tubulin and microtubule assembly was observed at the site of nuclear exchange during conjugation. This complexity accounts for the nucleation of most of the numerous and diverse microtubule arrays present in Paramecium. The sites and mode of nucleation of the microtubule bundles formed in the macronuclear compartment during division remain unclear. These observations lead us to discuss the relationships between microtubules, gamma-tubulin and MTOCs.

[1]  M. Gorovsky,et al.  Tetrahymena thermophila contains a conventional γ-tubulin that is differentially required for the maintenance of different microtubule-organizing centers , 2002, The Journal of cell biology.

[2]  M. Sauter,et al.  The plant Spc98p homologue colocalizes with gamma-tubulin at microtubule nucleation sites and is required for microtubule nucleation. , 2002, Journal of cell science.

[3]  Y. Nakajima,et al.  Microtubules Mediate Germ-nuclear Behavior after Meiosis in Conjugation of Paramecium caudatum , 2002, The Journal of eukaryotic microbiology.

[4]  I. Hagan,et al.  MTOC formation during mitotic exit in fission yeast. , 2001, Journal of cell science.

[5]  M. Snyder,et al.  Phosphorylation of γ-Tubulin Regulates Microtubule Organization in Budding Yeast , 2001 .

[6]  D. Agard,et al.  γ-Tubulin complexes and microtubule nucleation , 2001 .

[7]  R. Roberson,et al.  Microtubules Are required for motility and positioning of vesicles and mitochondria in hyphal tip cells of Allomyces macrogynus. , 2000, Fungal genetics and biology : FG & B.

[8]  O. Numata,et al.  Reorganization of microtubules in the amitotically dividing macronucleus of tetrahymena. , 2000, Cell motility and the cytoskeleton.

[9]  L. Bögre,et al.  Nuclear γ-Tubulin during Acentriolar Plant Mitosis , 2000, Plant Cell.

[10]  P. Ferrara,et al.  HUMAN 76P : A NEW MEMBER OF THE GAMMA -TUBULIN-ASSOCIATED PROTEIN FAMILY , 1999 .

[11]  B. Raynaud-Messina,et al.  Condensation-decondensation of the gamma-tubulin containing material in the absence of a structurally visible organelle during the cell cycle of Physarum plasmodia. , 1999, Biology of the cell.

[12]  Yixian Zheng,et al.  Gamma-tubulin complexes and their interaction with microtubule-organizing centers. , 1999, Current opinion in structural biology.

[13]  J. Rossier,et al.  Basal body duplication in Paramecium requires γ-tubulin , 1999, Current Biology.

[14]  J. Petersen,et al.  Conjugation in S. pombe: identification of a microtubule-organising centre, a requirement for microtubules and a role for Mad2 , 1998, Current Biology.

[15]  B. Alberts,et al.  Recruitment of the γ-Tubulin Ring Complex to Drosophila Salt-stripped Centrosome Scaffolds , 1998, The Journal of cell biology.

[16]  M. Knop,et al.  Receptors determine the cellular localization of a γ‐tubulin complex and thereby the site of microtubule formation , 1998, The EMBO journal.

[17]  T. Stearns,et al.  The Mammalian γ-Tubulin Complex Contains Homologues of the Yeast Spindle Pole Body Components Spc97p and Spc98p , 1998, The Journal of cell biology.

[18]  A. Iwamatsu,et al.  Xgrip109: A γ Tubulin–Associated Protein with an Essential Role in γ Tubulin Ring Complex (γTuRC) Assembly and Centrosome Function , 1998, The Journal of cell biology.

[19]  M. Bornens,et al.  Characterization of the Human Homologue of the Yeast Spc98p and Its Association with γ-Tubulin , 1998, The Journal of cell biology.

[20]  T. Stearns,et al.  Nucleation and capture of large cell surface‐associated microtubule arrays that are not located near centrosomes in certain cochlear epithelial cells , 1998, Journal of anatomy.

[21]  M. Bornens,et al.  Identification of an Spc110p-related protein in vertebrates. , 1997, Journal of cell science.

[22]  M. Schliwa,et al.  Unusual distribution of gamma-tubulin in the giant fresh water amoeba Reticulomyxa filosa. , 1997, European Journal of Cell Biology.

[23]  K. Gull,et al.  gamma-tubulin in trypanosomes: molecular characterisation and localisation to multiple and diverse microtubule organising centres. , 1997, Journal of cell science.

[24]  J. Beisson,et al.  Gamma-tubulin is permanently associated with basal bodies in ciliates. , 1996, European journal of cell biology.

[25]  H. Joshi,et al.  Tubulin transport in neurons , 1996, The Journal of cell biology.

[26]  M. Bornens,et al.  gamma-Tubulin in mammalian cells: the centrosomal and the cytosolic forms. , 1996, Journal of cell science.

[27]  Harish C. Joshi,et al.  gamma-tubulin is a minus end-specific microtubule binding protein , 1995, The Journal of cell biology.

[28]  Q. Li,et al.  A transient association of gamma-tubulin at the midbody is required for the completion of cytokinesis during the mammalian cell division. , 1995, Journal of cell science.

[29]  K. Gull,et al.  A molecular marker for centriole maturation in the mammalian cell cycle , 1995, The Journal of cell biology.

[30]  G. Géraud,et al.  Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells. , 1995, Journal of cell science.

[31]  F. Iftode,et al.  Where and when is microtubule diversity generated inParamecium? Immunological properties of microtubular networks in the interphase and dividing cells , 1995, Protoplasma.

[32]  C. Sunkel,et al.  Gamma‐tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts. , 1995, The EMBO journal.

[33]  A. Debec,et al.  Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindle. , 1994, Journal of cell science.

[34]  M. Kirschner,et al.  In vitro reconstitution of centrosome assembly and function: The central role of γ-tubulin , 1994, Cell.

[35]  H. Joshi,et al.  gamma-Tubulin in Arabidopsis: gene sequence, immunoblot, and immunofluorescence studies. , 1994, The Plant cell.

[36]  M. Félix,et al.  Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation , 1994, The Journal of cell biology.

[37]  A. Liang,et al.  The macronuclear gamma-tubulin-encoding gene of Euplotes octocarinatus contains two introns and an in-frame TGA. , 1993, Gene.

[38]  H. Joshi.  γ‐Tubulin: The hub of cellular microtubule assemblies , 1993, BioEssays : news and reviews in molecular, cellular and developmental biology.

[39]  V. Centonze,et al.  Microtubule nucleation and release from the neuronal centrosome , 1993, The Journal of cell biology.

[40]  H. Mazarguil,et al.  gamma-Tubulin participates in the formation of the midbody during cytokinesis in mammalian cells. , 1993, Journal of cell science.

[41]  C. Antony,et al.  gamma-Tubulin is present in acentriolar MTOCs during early mouse development. , 1993, Journal of cell science.

[42]  J. Besharse,et al.  Gamma-tubulin in differentiated cell types: localization in the vicinity of basal bodies in retinal photoreceptors and ciliated epithelia. , 1993, Journal of cell science.

[43]  A. Torres,et al.  Cortical development associated with conjugation of Paramecium , 1993 .

[44]  H. Joshi,et al.  Gamma-tubulin reorganization during mouse fertilization and early development. , 1993, Journal of cell science.

[45]  H. Joshi,et al.  Gamma-tubulin distribution in the neuron: implications for the origins of neuritic microtubules , 1992, The Journal of cell biology.

[46]  A. Adoutte,et al.  Cortical alveoli of Paramecium: a vast submembranous calcium storage compartment , 1991, The Journal of cell biology.

[47]  J. Beisson,et al.  Purification of the surface membrane-cytoskeleton complex (Cortex) of Paramecium and identification of several of its protein constituents. , 1990, European journal of protistology.

[48]  B. Oakley,et al.  Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans , 1989, Nature.

[49]  K. Weber,et al.  Turnover of the carboxy-terminal tyrosine of alpha-tubulin and means of reaching elevated levels of detyrosination in living cells. , 1987, Journal of cell science.

[50]  D. L. Roche,et al.  Spindle microtubule differentiation and deployment during micronuclear mitosis in Paramecium , 1985, The Journal of cell biology.

[51]  E. Hamilton,et al.  A microtubule meshwork associated with gametic pronucleus transfer across a cell-cell junction. , 1983, Science.

[52]  C. Petit,et al.  Immortalization of rodent embryo fibroblasts by SV40 is maintained by the A gene. , 1983, Virology.

[53]  J. Beisson,et al.  Positional control of nuclear differentiation in Paramecium. , 1981, Developmental biology.

[54]  J. Beisson,et al.  Microtubules and control of macronuclear 'amitosis' in Paramecium. , 1980, Journal of cell science.

[55]  A. Jurand.  Some Ultrastructural Features of Micronuclei during Conjugation and Autogamy in Paramecium aurelia , 1976 .

[56]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[57]  G. Selman,et al.  Ultrastructure of the Nuclei and Intranuclear Microtubules of Paramecium aurelia , 1970 .

[58]  S. S. Andersen,et al.  Molecular characteristics of the centrosome. , 1999, International review of cytology.

[59]  C. Silflow,et al.  Gamma-tubulin in Chlamydomonas: characterization of the gene and localization of the gene product in cells. , 1999, Cell motility and the cytoskeleton.

[60]  M. Snyder,et al.  γ-Tubulin of budding yeast , 1999 .

[61]  T. Stearns,et al.  Centrosomal deployment of gamma-tubulin and pericentrin: evidence for a microtubule-nucleating domain and a minus-end docking domain in certain mouse epithelial cells. , 1997, Cell motility and the cytoskeleton.

[62]  J. Beisson,et al.  Genetic evidence for a role of centrin-associated proteins in the organization and dynamics of the infraciliary lattice in Paramecium. , 1997, Cell Motility and the Cytoskeleton.

[63]  J. Beisson,et al.  The tubulin gene family of Paramecium: Characterization and expression of the αPT1 and αPT2 genes which code for α-tubulins with unusual C-terminal amino acids, GLY and ALA , 1996 .

[64]  J. Celis,et al.  Reference points for comparisons of two‐dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions , 1994, Electrophoresis.

[65]  M. Moudjou,et al.  Antibodies that can discriminate between dynein heavy chains and their HUV1 photoproducts. , 1994, Cell motility and the cytoskeleton.

[66]  K. Grell.  The Protozoan Nucleus , 1964 .