Physiological analysis of common bean (Phaseolus vulgaris L.) cultivars uncovers characteristics related to terminal drought resistance.

Terminal drought is a major problem for common bean production because it occurs during the reproductive stage, importantly affecting seed yield. Diverse common bean cultivars with different drought susceptibility have been selected from different gene pools in several drought environments. To better understand the mechanisms associated with terminal drought resistance in a particular common bean race (Durango) and growth habit (type-III), we evaluated several metabolic and physiological parameters using two cultivars, Bayo Madero and Pinto Saltillo, with contrasting drought susceptibility. The common bean cultivars were submitted to moderate and severe terminal drought treatments under greenhouse conditions. We analyzed the following traits: relative growth rate, photosynthesis and transpiration rates, stomatal conductance, water-use efficiency, relative water content, proline accumulation, glycolate oxidase activity and their antioxidant response. Our results indicate that the competence of the drought-resistant cultivar (Pinto Saltillo) to maintain seed production upon terminal drought relies on an early response and fine-tuning of stomatal conductance, CO₂ diffusion and fixation, and by an increased water use and avoidance of ROS accumulation.

[1]  J. Myers,et al.  Contributions of the Bean/Cowpea CRSP to cultivar and germplasm development in common bean , 2003 .

[2]  C. A. Jaleel,et al.  Understanding water deficit stress-induced changes in the basic metabolism of higher plants – biotechnologically and sustainably improving agriculture and the ecoenvironment in arid regions of the globe , 2009, Critical reviews in biotechnology.

[3]  J. Ruíz,et al.  Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. , 2010 .

[4]  L. Romero,et al.  Ammonia production and assimilation: its importance as a tolerance mechanism during moderate water deficit in tomato plants. , 2011, Journal of plant physiology.

[5]  P. M. Neumann.  Coping mechanisms for crop plants in drought-prone environments. , 2008, Annals of botany.

[6]  Shree P. Singh.  Broadening the Genetic Base of Common Bean Cultivars , 2001 .

[7]  D. Lawlor,et al.  Causes of Decreased Photosynthetic Rate and Metabolic Capacity in Water-deficient Leaf Cells: a Critical Evaluation of Mechanisms and Integration of Processes , 1996 .

[8]  Jean-François Ledent,et al.  Effect of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaseolus vulgaris L.) , 2007 .

[9]  J. Acosta-Gallegos,et al.  Improving Common Bean Performance under Drought Stress , 1997 .

[10]  Shree P. Singh,et al.  Registration of ‘Pinto Saltillo’ Common Bean , 2004 .

[11]  J. .. Padilla-Ramírez,et al.  ADAPTATION TRAITS IN DRY BEAN CULTIVARS GROWN UNDER DROUGHT STRESS , 2009 .

[12]  G. Farquhar,et al.  Water-use efficiency and carbon isotope discrimination in peanut under water deficit conditions , 1994 .

[13]  I. Fridovich,et al.  Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. , 1987, Analytical biochemistry.

[14]  Jiwu Zeng,et al.  Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice. , 2009, Journal of experimental botany.

[15]  Shree P. Singh.  Selection for Water‐Stress Tolerance in Interracial Populations of Common Bean , 1995 .

[16]  J. Flexas,et al.  Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. , 2009, Annals of botany.

[17]  H. Barrs,et al.  A Re-Examination of the Relative Turgidity Technique for Estimating Water Deficits in Leaves , 1962 .

[18]  K. Asada,et al.  THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons. , 1999, Annual review of plant physiology and plant molecular biology.

[19]  J. Kelly,et al.  Breeding for yield in dry bean (Phaseolus vulgaris L.) , 1998, Euphytica.

[20]  M. Bor,et al.  Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress , 2005 .

[21]  V. Velikova,et al.  Plant Responses to Drought, Acclimation, and Stress Tolerance , 2000, Photosynthetica.

[22]  H. Beevers,et al.  Developmental studies on microbodies in wheat leaves : I. Conditions influencing enzyme development. , 1972, Plant physiology.

[23]  J. Vanderleyden,et al.  Beans (Phaseolus spp.) – model food legumes , 2004, Plant and Soil.

[24]  R. Leegood,et al.  Photorespiration: metabolic pathways and their role in stress protection. , 2000, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[25]  R. Paquin,et al.  Observations sur une méthode de dosage de la proline libre dans les extraits de plantes , 1979 .

[26]  K. Basford,et al.  Genetic variability in cultivated common bean beyond the two major gene pools , 2004, Genetic Resources and Crop Evolution.

[27]  M. W. Adams,et al.  Plant traits and yield stability of dry bean (Phaseolus vulgaris) cultivars under drought stress , 1991, The Journal of Agricultural Science.

[28]  B. Osmond,et al.  Too many photons: photorespiration, photoinhibition and photooxidation , 1997 .

[29]  C. Foyer,et al.  Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context , 2005 .

[30]  Dae-Ok Kim,et al.  Antioxidant capacity of phenolic phytochemicals from various cultivars of plums , 2003 .

[31]  A. Covarrubias,et al.  Relationship between carbohydrate partitioning and drought resistance in common bean. , 2008, Plant, cell & environment.

[32]  Jeffrey W. White,et al.  Inheritance of seed yield, maturity and seed weight of common bean (Phaseolus vulgaris) under semi-arid rainfed conditions , 1994, The Journal of Agricultural Science.

[33]  O. Blokhina,et al.  Antioxidants, oxidative damage and oxygen deprivation stress: a review. , 2003, Annals of botany.

[34]  Shree P. Singh,et al.  Comparison of Sources and Lines Selected for Drought Resistance in Common Bean. , 2002, Crop science.

[35]  L. Romero,et al.  Photorespiration Process and Nitrogen Metabolism in Lettuce Plants (Lactuca sativa L.): Induced Changes in Response to Iodine Biofortification , 2010, Journal of Plant Growth Regulation.

[36]  J. Flexas,et al.  Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? , 2004, The New phytologist.

[37]  I. Rao,et al.  Phenotyping common beans for adaptation to drought , 2013, Front. Physiol..

[38]  H. Krüger,et al.  Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean , 2002 .

[39]  R. Dewar.  A simple model of light and water use evaluated for Pinus radiata. , 1997, Tree physiology.

[40]  James D. Kelly,et al.  Breeding beans for resistance to terminal drought in the Lowland tropics , 2005, Euphytica.

[41]  J. Kelly,et al.  Traits related to drought resistance in common bean , 1998, Euphytica.

[42]  C. Vance,et al.  Legumes: Importance and Constraints to Greater Use , 2003, Plant Physiology.

[43]  J. A. Gallegos,et al.  Effect of water stress on growth and yield of indeterminate dry-bean (Phaseolus vulgaris) cultivars , 1989 .

[44]  L. Romero,et al.  Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses. , 2009 .

[45]  K. Asada,et al.  Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts , 1981 .

[46]  R. Mittler.  Oxidative stress, antioxidants and stress tolerance. , 2002, Trends in plant science.

[47]  J. Whitmore.  Plant Responses to Drought , 2000 .

[48]  A. Blum.  Drought resistance, water-use efficiency, and yield potential-are they compatible, dissonant, or mutually exclusive? , 2005 .

[49]  G. Paliyath,et al.  Ultraviolet-B- and Ozone-Induced Biochemical Changes in Antioxidant Enzymes of Arabidopsis thaliana , 1996, Plant physiology.

[50]  J. Acosta-Gallegos,et al.  Biomass distribution, maturity acceleration and yield in drought-stressed common bean cultivars , 2004 .

[51]  J. Ruíz,et al.  Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation , 2006 .

[52]  Qun Sun,et al.  Dynamic changes of anti-oxidative enzymes of 10 wheat genotypes at soil water deficits. , 2005, Colloids and surfaces. B, Biointerfaces.

[53]  C. Abdelly,et al.  Effect of water stress on growth, osmotic adjustment, cell wall elasticity and water-use efficiency in Spartina alterniflora , 2009 .

[54]  L. Romero,et al.  Proline metabolism in cherry tomato exocarp in relation to temperature and solar radiation , 2007 .

[55]  J. Staden,et al.  Dissecting the roles of osmolyte accumulation during stress , 1998 .

[56]  J. Feierabend.  Developmental studies on microbodies in wheat leaves , 2004, Planta.

[57]  M. Pastor-Corrales,et al.  Andean beans (Phaseolus vulgaris L.) with resistance to the angular leaf spot pathogen (Phaeoisariopsis griseola) in southern and eastern Africa , 2004, Euphytica.

[58]  S. Zhao,et al.  Photoprotective Function of Photorespiration in Several Grapevine Cultivars Under Drought Stress , 2004, Photosynthetica.

[59]  Matthew W. Blair,et al.  Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding , 2006, Euphytica.

[60]  P. Hare.  Review article. Proline synthesis and degradation: a model system for elucidating stress-related signal transduction , 1999 .

[61]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[62]  Wilfried Claussen,et al.  Proline as a measure of stress in tomato plants , 2005 .

[63]  M. Okamura,et al.  An improved method for determination of L-ascorbic acid and L-dehydroascorbic acid in blood plasma. , 1980, Clinica chimica acta; international journal of clinical chemistry.

[64]  L. Romero,et al.  Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. , 2001, Plant science : an international journal of experimental plant biology.