Primary structure and expression of a naturally truncated human P2X ATP receptor subunit from brain and immune system

A novel member of the ionotropic ATP receptor gene family has been identified in human brain. This 422 amino acid long P2X receptor subunit has 62% sequence identity with rat P2X5. Several characteristic motifs of ATP‐gated channels are present in its primary structure, but this P2X5‐related subunit displays a single transmembrane domain. Heterologous expression of chimeric subunits containing the C‐terminal domain of rat P2X5 leads to the formation of desensitizing functional ATP‐gated channels in Xenopus oocytes. The developmentally regulated mRNA, found in two splicing variant forms, is expressed at high levels in brain and immune system.

[1]  P. Séguéla,et al.  A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain , 1996, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[2]  G. Buell,et al.  P2X Receptors: An Emerging Channel Family , 1996, The European journal of neuroscience.

[3]  R. North,et al.  Identification of amino acid residues contributing to the pore of a P2X receptor , 1997, The EMBO journal.

[4]  G. Burnstock,et al.  A P2X purinoceptor expressed by a subset of sensory neurons , 1995, Nature.

[5]  J. Cohen,et al.  Identification of mRNAs associated with programmed cell death in immature thymocytes , 1991, Molecular and cellular biology.

[6]  T. Heidmann,et al.  mRNA retroposition in human cells: processed pseudogene formation. , 1995, The EMBO journal.

[7]  Caterina Virginio,et al.  The Permeabilizing ATP Receptor, P2X7 , 1997, The Journal of Biological Chemistry.

[8]  King Lb,et al.  Thymocyte and T cell apoptosis: is all death created equal? , 1994 .

[9]  R. North,et al.  An antagonist‐insensitive P2X receptor expressed in epithelia and brain. , 1996, The EMBO journal.

[10]  R. North,et al.  Domains of P2X receptors involved in desensitization. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[11]  E. Kawashima,et al.  The Cytolytic P2Z Receptor for Extracellular ATP Identified as a P2X Receptor (P2X7) , 1996, Science.

[12]  D. Julius,et al.  New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor , 1994, Nature.

[13]  C. Auffray,et al.  The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. , 1996, Genomics.

[14]  P. A. Goldstein,et al.  ATP P2X Receptors Mediate Fast Synaptic Transmission in the Dorsal Horn of the Rat Spinal Cord , 1997, The Journal of Neuroscience.

[15]  R. North,et al.  A new class of ligand-gated ion channel defined by P2X receptor for extracellular ATP , 1994, Nature.

[16]  W. Stühmer,et al.  Characterization of recombinant human P2X4 receptor reveals pharmacological differences to the rat homologue. , 1997, Molecular pharmacology.

[17]  W. Stühmer,et al.  Molecular cloning and functional expression of a novel rat heart P2X purinoceptor , 1996, FEBS letters.

[18]  W. Stühmer,et al.  P2X4: an ATP-activated ionotropic receptor cloned from rat brain. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[19]  P. Séguéla,et al.  Immunological Characterization and Transmembrane Topology of 5‐Hydroxytryptamine3 Receptors by Functional Epitope Tagging , 1996, Journal of neurochemistry.

[20]  D. Bertrand,et al.  Electrophysiology of Neuronal Nicotinic Acetylcholine Receptors Expressed in Xenopus Oocytes following Nuclear Injection of Genes or cDNAs , 1991 .

[21]  Brock L. Eide,et al.  Naturally Occurring Truncated trkB Receptors Have Dominant Inhibitory Effects on Brain-Derived Neurotrophic Factor Signaling , 1996, The Journal of Neuroscience.

[22]  R. North,et al.  Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons , 1995, Nature.

[23]  W. Stühmer,et al.  Molecular characterization and pharmacological properties of the human P2X3 purinoceptor. , 1997, Brain research. Molecular brain research.

[24]  R. North,et al.  Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels , 1996, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[25]  M. Barbacid.  The Trk family of neurotrophin receptors. , 1994, Journal of neurobiology.

[26]  S. Antonarakis,et al.  Characterization and chromosomal localization of a human P2X receptor from the urinary bladder. , 1995, Receptors & channels.

[27]  M. Jiang,et al.  Suppression of Slow Delayed Rectifier Current by a Truncated Isoform of KvLQT1 Cloned from Normal Human Heart* , 1997, The Journal of Biological Chemistry.