Glasses having a chemical composition between 1Na2O–2CaO–3SiO2 (1N2C3S) and 1.5Na2O–1.5CaO–3SiO2, containing 0, 2, 4 and 6 wt% P2O5, were crystallized to several volume percent through thermal treatments in the range 550–700 °C. These glasses and glass-ceramics were exposed to a simulated body fluid solution (SBF-K9 which is close to human plasma) for several time periods. Fourier transform infrared spectroscopy (FTIR) was used to determine the rate of hydroxy carbonate apatite (HCA) formation. Crystallization decreased the kinetics but did not inhibit the development of a HCA layer, even in fully crystallized ceramics. The onset time for crystallization of HCA varied from 8 h for a glass containing 6% P2O5 to 35 h for a fully crystallized 1.07Na2O–2CaO–3SiO2 ceramic. The HCA layer formation of these compositions in `in vitro' tests is much faster than in commercial bioactive materials such as synthetic hydroxyapatite ceramic, A/W glass-ceramic, Ceravital and Bioverit, for which the onset time usually takes at least seven days. FTIR and inductive coupled plasma studies confirmed the formation of an apatite layer which indicates bioactivity in the 1N2C3S crystal phase. X-ray diffraction experiments show that the phosphorus ions are kept in solid solution in the crystal phase. An apatite-like compound only appeared when the specimens were submitted to very long additional thermal treatments. The bioactivity of commercial materials is based on the apatite crystal phase, while the high level of bioactivity of this new generation of glass-ceramics is attained due to the combination of two mechanisms acting simultaneously; a non-phosphate bioactive crystal phase (1N2C3S) and the phosphorus ions in solid solution which are easily released from the structure, promoting a faster HCA layer formation similar to 45S5 Bioglass®.
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