Thermal and physicochemical properties of red tilapia (Oreochromis niloticus) surimi gel as affected by microbial transglutaminase

Thermal and physicochemical properties of red tilapia (Oreochromis niloticus) surimi gel incorporated with different levels of microbial transglutaminase (MTGase) were investigated. Surimi samples mixed with various concentrations of MTGase were subjected to two-stages heating (at 45°C followed by 90°C) to prepare surimi gel. Samples formulated with 0.30 MTGase (units/g surimi) showed the highest breaking force and deformation, and lowest expressible water content among treatments. Highest storage modulus was found in the gels mixed with 0.30 MTGase (units/g surimi). Compared with control surimi gel, addition of microbial transglutaminase to levels 0.10, 0.20 and 0.30 (units/g surimi) increased the enthalpy and maximum transition temperature of myosin. Results suggest that up to 0.30 MTGase (units/g surimi) could improve texture, colour, water-holding capacity, elasticity and thermal stability of red tilapia surimi gel.

[1]  M. Chaijan,et al.  Gel-Forming Ability of Mackerel (Rastrelliger Branchysoma) Protein Isolate as Affected by Microbial Transglutaminase , 2011 .

[2]  C. M Lee,et al.  Surimi process technology , 1984 .

[3]  J. Yongsawatdigul,et al.  Effect of Endogenous Transglutaminase on Threadfin Bream Surimi Gelation , 2002 .

[4]  K. Duangmal,et al.  Effect of protein additives, sodium ascorbate, and microbial transglutaminase on the texture and colour of red tilapia surimi gel. , 2009 .

[5]  W. Visessanguan,et al.  Transglutaminase-mediated setting in bigeye snapper Surimi , 2003 .

[6]  W. Visessanguan,et al.  Gel‐forming properties of surimi produced from bigeye snapper, Priacanthus tayenus and P macracanthus, stored in ice , 2002 .

[7]  Tao Wu,et al.  Gelling properties and lipid oxidation of kamaboko gels from grass carp (Ctenopharyngodon idellus) influenced by chitosan , 2007 .

[8]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[9]  J. Torres,et al.  Effect of adding insoluble solids from surimi wash water on the functional and mechanical properties of pacific whiting grade A surimi. , 2007, Bioresource technology.

[10]  K. Yokoyama,et al.  Properties and applications of microbial transglutaminase , 2004, Applied Microbiology and Biotechnology.

[11]  C. S. Cheow,et al.  THE EFFECT OF PRESSURE COOKING ON THE MICROSTRUCTURE AND EXPANSION OF FISH CRACKER (‘KEROPOK’) , 2001 .

[12]  S. Bogason,et al.  Changes in myofibrillar proteins during processing of salted cod (Gadus morhua) as determined by electrophoresis and differential scanning calorimetry , 2002 .

[13]  J. A. Ramírez,et al.  Surimi gels from striped mullet (Mugil cephalus) employing microbial transglutaminase , 2000 .

[14]  Shann-Tzong Jiang,et al.  Microbial Transglutaminase Affects Gel Properties of Golden Threadfin-bream and Pollack Surimi , 2000 .

[15]  C. Spink Differential scanning calorimetry. , 2008, Methods in cell biology.

[16]  S. Benjakul,et al.  Effect of bovine and fish gelatin in combination with microbial transglutaminase on gel properties of threadfin bream surimi , 2012, International Aquatic Research.

[17]  Chiya Kuraishi,et al.  Production of Restructured Meat using Microbial Transglutaminase without Salt or Cooking , 1997 .

[18]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[19]  J. Park Ingredient Technology for Surimi and Surimi Seafood , 2005 .

[20]  K. Hsu,et al.  Changes in conformation and sulfhydryl groups of tilapia actomyosin by thermal treatment , 2007 .

[21]  J. Yongsawatdigul,et al.  Effect of microbial transglutaminase on autolysis and gelation of lizardfish surimi , 2005 .

[22]  D. Petridis,et al.  The Effect of Washing, Microbial Transglutaminase, Salts and Starch Addition on the Functional Properties of Sardine (Sardina Pilchardus) Kamaboko Gels , 2008 .

[23]  Soottawat Benjakul,et al.  Characterization of edible films from skin gelatin of brownstripe red snapper and bigeye snapper , 2006 .

[24]  K. Chin,et al.  Effects of fish sarcoplasmic proteins on the properties of myofibrillar protein gels mediated by microbial transglutaminase , 2013 .

[25]  M. Mordi,et al.  Characterization of fish gelatin from surimi processing wastes: Thermal analysis and effect of transglutaminase on gel properties , 2009 .

[26]  W. Visessanguan,et al.  Effect of phosphate compounds on gel-forming ability of surimi from bigeye snapper (Priacanthus tayenus) , 2006 .

[27]  M. Nunes,et al.  Effect of salt and MTGase on the production of high quality gels from farmed sea bass , 2010 .

[28]  A. H-Kittikun,et al.  Comparative study on protein cross-linking and gel enhancing effect of microbial transglutaminase on surimi from different fish. , 2012, Journal of the science of food and agriculture.

[29]  H. Ockerman,et al.  The Effects of Egg White, Tumbling and Storage Time on Proximate Composition and Protein Fractions of Restructured Fish Product , 1995 .

[30]  S. Rawdkuen,et al.  Rheological and Textural Properties of Pacific Whiting Surimi Gels As Influenced by Chicken Plasma , 2008 .

[31]  J. Park,et al.  Surimi: manufacturing and evaluation. , 2005 .

[32]  George I Makhatadze,et al.  Differential Scanning Calorimetry , 2009 .

[33]  S. Benjakul,et al.  Properties of surimi gel as influenced by fish gelatin and microbial transglutaminase , 2013 .

[34]  Tao Yin,et al.  Effects of nano-scaled fish bone on the gelation properties of Alaska pollock surimi. , 2014, Food chemistry.

[35]  S. Rawdkuen,et al.  Biochemical and gelling properties of tilapia surimi and protein recovered using an acid-alkaline process , 2009 .

[36]  E. Curotto,et al.  Transglutaminase effects on gelation capacity of thermally induced beef protein gels , 2006 .

[37]  K. Pan,et al.  Cryoprotective effects of trehalose and sodium lactate on tilapia (Sarotherodon nilotica) surimi during frozen storage , 2006 .

[38]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[39]  W. Visessanguan,et al.  Effect of frozen storage on chemical and gel-forming properties of fish commonly used for surimi production in Thailand , 2005 .

[40]  D. D. Hamann,et al.  Transglutaminase effects on low temperature gelation of fish protein sols , 1997 .

[41]  J. W. Park,et al.  Physical Characteristics of Surimi Seafood as Affected By Thermal Processing Conditions , 1999 .

[42]  W. Visessanguan,et al.  Gelling properties of white shrimp (Penaeus vannamei) meat as influenced by setting condition and microbial transglutaminase , 2007 .

[43]  J. Morikawa,et al.  Effect of amino acids on the eutectic behavior of NaCl solutions studied by DSC. , 2005, Cryobiology.