Clinical versatility of porcine hepatocytes in the light of interspecies differences in cytochrome P450 regulation and expression

Abstract:  Background and aims:  In fulminant hepatic failure, the clinical use of bioartifical liver support with porcine hepatocytes is the subject of a controversial debate. Cytochrome P450 (CYP) metabolic functions have relevant implications for drug metabolism and detoxification. In this study, we investigate interspecies differences in CYP gene expression between human and porcine primary hepatocytes and the impact of interleukin 6 (IL‐6) and tumor necrosis factor alpha (TNF‐α) exposition mimicking cytokine release in fulminant hepatic failure.

[1]  H. Schrem,et al.  Physiological incompatibilities of porcine hepatocytes for clinical liver support , 2006, Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society.

[2]  M. Skaanild.  Porcine cytochrome P450 and metabolism. , 2006, Current pharmaceutical design.

[3]  O. Schmitz,et al.  Evidence that acute hyperinsulinaemia increases the cytokine content in essential organs after an endotoxin challenge in a porcine model , 2005, Acta anaesthesiologica Scandinavica.

[4]  J. Gjedsted,et al.  Inflammatory response during hyperglycemia and hyperinsulinemia in a porcine endotoxemic model: the contribution of essential organs , 2005, Acta anaesthesiologica Scandinavica.

[5]  J. O'grady.  Bioartificial liver in acute liver failure: Impostor or simply misunderstood? , 2005, Hepatology.

[6]  D. Samuel,et al.  Treatment of patients with hepatic failure: the difficult place of liver support systems. , 2004, Journal of hepatology.

[7]  Mauro Salizzoni,et al.  Prospective, Randomized, Multicenter, Controlled Trial of a Bioartificial Liver in Treating Acute Liver Failure , 2004, Annals of surgery.

[8]  T. Kamataki,et al.  Cloning CYP2D21 and CYP3A22 cDNAs from liver of miniature pigs. , 2004, Drug metabolism and disposition: the biological fate of chemicals.

[9]  T. Hansen,et al.  DNA adducts in cultures of polychlorinated biphenyl-treated human hepatocytes. , 2003, Toxicology and applied pharmacology.

[10]  P. Vierling,et al.  Prodrugs of HIV protease inhibitors. , 2003, Current pharmaceutical design.

[11]  A. Dhawan,et al.  Progress in human hepatocytes: isolation, culture & cryopreservation. , 2002, Seminars in cell & developmental biology.

[12]  J. Castell,et al.  Down‐regulation of human CYP3A4 by the inflammatory signal interleukin 6: molecular mechanism and transcription factors involved , 2002, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[13]  J. Vienken,et al.  Extracorporeal Liver Support: Porcine or Human Cell Based Systems? , 2002, The International journal of artificial organs.

[14]  K. Olthoff,et al.  Interleukin‐6 from intrahepatic cells of bone marrow origin is required for normal murine liver regeneration , 2002, Hepatology.

[15]  D. Farrell,et al.  Identification of multiple constitutive and inducible hepatic cytochrome P450 enzymes in market weight swine. , 2001, Drug metabolism and disposition: the biological fate of chemicals.

[16]  M. Manns,et al.  Interleukin-6-induced Tethering of STAT3 to the LAP/C/EBPβ Promoter Suggests a New Mechanism of Transcriptional Regulation by STAT3* , 2001, The Journal of Biological Chemistry.

[17]  V. Armstrong,et al.  Preliminary report on the effect of xenoperfusion with human blood on cyclosporin A metabolism and cytochrome-P-4503A4-mRNA expression in a pig liver perfusion model. , 2001, Clinical biochemistry.

[18]  H. Schrem,et al.  Liver Transplantation After Organ Preservation With Normothermic Extracorporeal Perfusion , 2001, Annals of surgery.

[19]  D. Sudan,et al.  EXTRACORPOREAL LIVER PERFUSION USING HUMAN AND PIG LIVERS FOR ACUTE LIVER FAILURE , 2000, Transplantation.

[20]  J. Pascussi,et al.  Interleukin-6 negatively regulates the expression of pregnane X receptor and constitutively activated receptor in primary human hepatocytes. , 2000, Biochemical and biophysical research communications.

[21]  M. Manns,et al.  Tumor necrosis factor α in the pathogenesis of human and murine fulminant hepatic failure , 2000 .

[22]  H. Schouten,et al.  Kupffer cell depletion by CI2MDP-liposomes alters hepatic cytokine expression and delays liver regeneration after partial hepatectomy. , 2000, Liver.

[23]  R. Weaver,et al.  Determining the best animal model for human cytochrome P450 activities: a comparison of mouse, rat, rabbit, dog, micropig, monkey and man , 2000, Xenobiotica; the fate of foreign compounds in biological systems.

[24]  N. Milošević,et al.  Kupffer cell-mediated differential down-regulation of cytochrome P450 metabolism in rat hepatocytes. , 1999, European journal of pharmacology.

[25]  R. Hughes,et al.  Plasma cytokine levels and coagulation and complement activation during use of the extracorporeal liver assist device in acute liver failure. , 1998, Artificial organs.

[26]  M. Fredholm,et al.  Mapping of porcine genes belonging to two different cytochrome P450 subfamilies. , 1998, Animal genetics.

[27]  J Kvĕtina,et al.  Presence and activity of cytochrome P450 isoforms in minipig liver microsomes. Comparison with human liver samples. , 1998, Drug metabolism and disposition: the biological fate of chemicals.

[28]  T. Kamataki,et al.  Regulation of CYP1A and CYP3A mRNAs by ascorbic acid in guinea pigs. , 1997, Archives of biochemistry and biophysics.

[29]  D. Abendroth,et al.  Immunopathological observations after xenogeneic liver perfusions using donor pigs transgenic for human decay-accelerating factor. , 1997, Transplantation.

[30]  S. Abrignani.  Bystander Activation by Cytokines of Intrahepatic T Cells in Chronic Viral Hepatitis , 1997, Seminars in liver disease.

[31]  C. Deutschman,et al.  Acute-phase gene expression correlates with intrahepatic tumor necrosis factor-alpha abundance but not with plasma tumor necrosis factor concentrations during sepsis/systemic inflammatory response syndrome in the rat. , 1996, Critical care medicine.

[32]  R. Witkamp,et al.  A lipopolysaccharide-induced acute phase response in the pig is associated with a decrease in hepatic cytochrome P450-mediated drug metabolism. , 1996, Journal of veterinary pharmacology and therapeutics.

[33]  P. Neuhaus,et al.  The release of cytokines, adhesion molecules, and extracellular matrix parameters during and after reperfusion in human liver transplantation. , 1996, Transplantation.

[34]  P. Maurel,et al.  Differential effects of cytokines on the inducible expression of CYP1A1, CYP1A2, and CYP3A4 in human hepatocytes in primary culture , 1995, Hepatology.

[35]  G. Missale,et al.  [Cytokine mediators in acute inflammation and chronic course of viral hepatitis]. , 1995, Annali italiani di medicina interna : organo ufficiale della Societa italiana di medicina interna.

[36]  H. Yamazaki,et al.  Interactions of ingested food, beverage, and tobacco components involving human cytochrome P4501A2, 2A6, 2E1, and 3A4 enzymes. , 1994, Environmental health perspectives.

[37]  Mehmet Toner,et al.  A Stable Long‐Term Hepatocyte Culture System for Studies of Physiologic Processes: Cytokine Stimulation of the Acute Phase Response in Rat and Human Hepatocytes , 1992, Biotechnology progress.

[38]  B. Beutler,et al.  Porcine tumor necrosis factor alpha: cloning with the polymerase chain reaction and determination of the nucleotide sequence. , 1989, Gene.

[39]  R. Tompkins,et al.  Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration , 1989, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[40]  D. Nebert,et al.  Human debrisoquine 4-hydroxylase (P450IID1): cDNA and deduced amino acid sequence and assignment of the CYP2D locus to chromosome 22. , 1988, Genomics.