The effect of multiple host contacts on the infectivity of dengue-2 virus-infected Aedes aegypti.

This study was designed to determine if transmission rates for dengue-2 virus by Aedes aegypti are altered by mosquitos probing a host for blood or imbibing blood prior to attempting transmission. Aedes aegypti is known to contact multiple hosts during each egg-laying cycle and multiple host contacts might diminish the amount of virus in infected mosquito's salivary glands or ducts and render them functionally uninfective. Probing a host 5, 10, or 20 consecutive times did not significantly alter the infectivity of parenterally infected mosquitoes. However, orally infected Ae. aegypti that probed 20 times transmitted dengue viruses at a significantly higher rate than controls. Infectivity of orally infected Ae. aegypti was unaffected by blood feeding. Our data suggest that (1) dengue virus-infected Ae. aegypti remain infective regardless of their probing or engorging history, and (2) once Ae. aegypti become infective they are extremely efficient disseminators of dengue virus.

[1]  Richard DiCarlo,et al.  Introduction to Biostatistics , 1996 .

[2]  T. Scott,et al.  Reproductive fitness and survivorship of Aedes aegypti (Diptera: Culicidae) maintained on blood, with field observations from Thailand. , 1994, Journal of medical entomology.

[3]  T. Scott,et al.  Female Aedes aegypti (Diptera: Culicidae) in Thailand rarely feed on sugar. , 1992, Journal of medical entomology.

[4]  J. Edman,et al.  Multiple transmission of Plasmodium gallinaceum (Eucoccida: Plasmodiidae) during serial probing by Aedes aegypti (Diptera: Culicidae) on several hosts. , 1992, Journal of medical entomology.

[5]  R. Rosenberg,et al.  An estimation of the number of malaria sporozoites ejected by a feeding mosquito. , 1990, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[6]  E. Lennette,et al.  Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections , 1989 .

[7]  W. Hausermann,et al.  Dispersal and other population parameters of Aedes aegypti in an African village and their possible significance in epidemiology of vector-borne diseases. , 1986, The American journal of tropical medicine and hygiene.

[8]  B. Miller,et al.  Dengue-2 vaccine: oral infection, transmission, and lack of evidence for reversion in the mosquito, Aedes aegypti. , 1982, The American journal of tropical medicine and hygiene.

[9]  M. Klowden,et al.  Effect of defensive host behavior on the blood meal size and feeding success of natural populations of mosquitoes (Diptera: Culicidae). , 1979, Journal of medical entomology.

[10]  L. Rosen,et al.  A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. , 1977, The American journal of tropical medicine and hygiene.

[11]  D. Gubler,et al.  The use of mosquitoes to detect and propagate dengue viruses. , 1974, The American journal of tropical medicine and hygiene.

[12]  M. Yasuno,et al.  Field studies on the gonotrophic cycle of Aedes aegypti in Bangkok, Thailand. , 1973, Journal of medical entomology.

[13]  J. C. Jones,et al.  A qualitative method for estimating the degree of engorgement of Aedes aegypti adults. , 1972, Journal of medical entomology.

[14]  G. Mcclelland,et al.  Frequency of Blood Feeding in the Mosquito Aedes aegypti , 1971, Nature.

[15]  Daniel J. Gould,et al.  Ecology and control of dengue vectors on an island in the Gulf of Thailand. , 1970, Journal of medical entomology.

[16]  P. M. Sheppard,et al.  The dynamics of an adult population of Aedes aegypti in relation to dengue haemorrhagic fever in Bangkok. , 1969 .

[17]  H. S. Hurlbut.  Mosquito salivation and virus transmission. , 1966, The American journal of tropical medicine and hygiene.

[18]  D. A. Eliason.  Feeding Adult Mosquitoes on Solid Sugars , 1963, Nature.

[19]  Sir Rickard Christophers.  Aëdes Aegypti The Yellow Fever Mosquito: Its Life History, Bionomics and Structure , 1960 .

[20]  T. Scott,et al.  Detection of multiple blood feeding in Aedes aegypti (Diptera: Culicidae) during a single gonotrophic cycle using a histologic technique. , 1993, Journal of medical entomology.

[21]  T. Aitken.  An In vitro feeding technique for artificially demonstrating virus transmission by mosquitoes. , 1977 .

[22]  D. Gubler,et al.  A simple technique for demonstrating transmission of dengue virus by mosquitoes without the use of vertebrate hosts. , 1976, The American journal of tropical medicine and hygiene.

[23]  R. Tonn,et al.  A study of biting habits of Aedes aegypti in Bangkok, Thailand. , 1970, Bulletin of the World Health Organization.