Stimulation of PancreaticEnzyme Secretion by a Peptide Purifiedfrom Rat Bile-PancreaticJuice

We purified a peptide from rat pancreatic juice that enhances pancreatic enzyme secretion in a manner different from that of exogenous trypsin inhibitors, such as soybean trypsin inhibitor, when it is infused into the rat intestine. In this paper, we present evidence for the release of gut hormone(s) into the blood of rats after intestinal administration of the peptide. In the presence of atropine, an anesthetized rat small intestine was washed out with saline con taining soybean trypsin inhibitor (Kunitz type) to eliminate proteases. Under these conditions, the rat small intestine was divided into four equal parts by ligation. Administration of the peptide into the first quarter of the small intestine stimulated pancreatic enzyme secretion. However, administrations into the more distal parts did not stimulate the enzyme secretion. Moreover, intravenous injec tion of 1 mL of plasma from rats in which the peptide had been infused into the duodenum caused stimulation of pancreatic enzyme secretion in the recipient rats. It was suggested that the purified peptide acts in the proximal small intestine and that it stimulates the release of gut hormone(s) into the blood to enhance pancreatic enzyme secretion. These findings support the hypothesis that the peptide we purified is responsible in part for the humoral control of pancreatic enzyme secretion in the response to food intake. J. Nutr. 116: 1540-1546,