Biodegradation of Benzene by Alkaliphilic bacteria under Aerobic conditions

The aerobic biodegradation of benzene in laboratory cultures have been well studied, by using alkaliphilic bacteria strain Bacillus badius D1, The bacterial strain Bacillus badius D1 could complete degradation of benzene at a concentration of 5 ml/100 ml at pH 9.0 within 48 hrs, The metabolites of aerobic degradation of benzene by this strain were identified as Catechol, 2-hydroxymuconic semialdehyde and 2hydroxypenta-2,4-dienoic acid. The structural determination of the intermediates of benzene degradation was carried out by spectroscopic analysis like GC-MS chromatography and FTIR spectra. A tentative pathway of benzene degradation is also reported. To our knowledge, this is the first report that the isolated bacterial culture of Bacillus badius D1can capable to degraded benzene under alkaline conditions.

[1]  V. Sarwade,et al.  Degradation of Anthracene by Alkaliphilic Bacteria Bacillus badius , 2012 .

[2]  H. Hassan,et al.  Isolation and Characterization of Halophilic Aromatic and Chloroaromatic Degrader from Wadi El-Natrun Soda lakes , 2012 .

[3]  S. Radwan,et al.  Biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the Arabian Gulf , 2010, Extremophiles.

[4]  E. Madsen,et al.  Influence of Soil Components on the Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Pseudoxanthomonas spadix BD-a59 , 2008, Applied and Environmental Microbiology.

[5]  A. Stams,et al.  Isolation and Characterization of Alicycliphilus denitrificans Strain BC, Which Grows on Benzene with Chlorate as the Electron Acceptor , 2008, Applied and Environmental Microbiology.

[6]  K. Timmis,et al.  Isolation of alkali‐tolerant benzene‐degrading bacteria from a contaminated aquifer , 2008, Letters in applied microbiology.

[7]  C. Leyval,et al.  Real-Time PCR quantification of PAH-ring hydroxylating dioxygenase (PAH-RHDalpha) genes from Gram positive and Gram negative bacteria in soil and sediment samples. , 2008, Journal of microbiological methods.

[8]  K. Demnerova,et al.  Bacterial aerobic degradation of benzene, toluene, ethylbenzene and xylene , 2008, Folia Microbiologica.

[9]  C. Jeon,et al.  Isolation and Characterization of a New Benzene, Toluene, and Ethylbenzene Degrading Bacterium, Acinetobacter sp. B113 , 2008, Current Microbiology.

[10]  B. Mahendran,et al.  Modeling of growth kinetics for Pseudomonas spp. during benzene degradation , 2005, Applied Microbiology and Biotechnology.

[11]  B. Fathepure,et al.  Biodegradation of Benzene by Halophilic and Halotolerant Bacteria under Aerobic Conditions , 2004, Applied and Environmental Microbiology.

[12]  Mark R. Smith.  The biodegradation of aromatic hydrocarbons by bacteria , 2004, Biodegradation.

[13]  R. Cowan,et al.  Substrate inhibition kinetics for toluene and benzene degrading pure cultures and a method for collection and analysis of respirometric data for strongly inhibited cultures , 2003, Biotechnology and bioengineering.

[14]  J. G. Leahy,et al.  Degradation of mixtures of aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbon-degrading bacteria. , 2003, FEMS microbiology ecology.

[15]  D. C. Mosteller,et al.  Biodegradation kinetics of aromatic hydrocarbon mixtures by pure and mixed bacterial cultures. , 2002, Environmental health perspectives.

[16]  T. Omori,et al.  Bacterial degradation of aromatic compounds via angular dioxygenation. , 2001, The Journal of general and applied microbiology.

[17]  S. Leite,et al.  Gasoline biodegradation in different soil microcosms , 2000 .

[18]  Y. Yoo,et al.  Removal of benzene in a hybrid bioreactor , 1999 .

[19]  A. Borg-Karlson,et al.  Biological degradation of diesel fuel in water and soil monitored with solid-phase micro-extraction and GC-MS , 1998, Applied Microbiology and Biotechnology.

[20]  P. Barbieri,et al.  Cloning of the genes for and characterization of the early stages of toluene and o-xylene catabolism in Pseudomonas stutzeri OX1 , 1996, Applied and environmental microbiology.

[21]  M. E. Meek,et al.  Benzene: Evaluation of risks to health from environmental exposure in Canada , 1994 .

[22]  J M Tiedje,et al.  Isolation, characterization, and distribution of denitrifying toluene degraders from a variety of habitats , 1994, Applied and environmental microbiology.

[23]  M. Takagi,et al.  Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida , 1987, Journal of bacteriology.

[24]  B. J. Dean.  Recent findings on the genetic toxicology of benzene, toluene, xylenes and phenols. , 1985, Mutation research.