Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

In the present study, the synergistic protective effect of co-supplementation of glutathione (GSH) with selenium nanoparticles (SeNPs) on the cryopreservation efficiency of bull semen was analyzed.After collection, the ejaculates of Holstein bulls were subsequently diluted with a Tris extender buffer supplemented with different concentrations of SeNPs (0, 1, 2, and 4 μg/ml), followed by semen equilibration at 4°C and assessment of sperm viability and motility. Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 μg/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 μg/ml SeNPs (GSH + SeNPs group). After cryopreservation, motility, viability, mitochondrial activity, plasma membrane integrity, acrosome integrity, concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), and ability of frozen-thawed sperm cells to support in vitro embryonic development were evaluated.No side effect of SeNPs concentrations applied in the current study on the motility and viability of equilibrated bull spermatozoa was found. Meanwhile, supplementation of SeNPs significantly promoted the motility and viability of equilibrated bull spermatozoa. Furthermore, the co-supplementation of GSH with SeNPs effectively protected bull spermatozoa from cryoinjury as expressed by promoting semen motility, viability, mitochondrial activity, plasma membrane integrity, and acrosome integrity. Finally, the enhanced antioxidant capacity and embryonic development potential in the frozen-thawed bull spermatozoa cryopreserved by co-supplementation of GSH with SeNPs further confirmed the synergistic protective effect of co-supplementation of GSH with SeNPs on the cryopreservation of bull semen.

[1]  M. F. Akhtar,et al.  Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology , 2022, Animals : an open access journal from MDPI.

[2]  Liya Su,et al.  Beneficial Effect of Selenium Doped Carbon Quantum Dots Supplementation on the in vitro Development Competence of Ovine Oocytes , 2022, International journal of nanomedicine.

[3]  Feriel Yasmine Mahiddine,et al.  Commensal Lactobacilli Enhance Sperm Qualitative Parameters in Dogs , 2022, Frontiers in Veterinary Science.

[4]  Z. Rashidi,et al.  Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring , 2022, Journal of Assisted Reproduction and Genetics.

[5]  P. Sutovsky,et al.  Potential Use of Tannin Extracts as Additives in Semen Destined for Cryopreservation: A Review , 2022, Animals : an open access journal from MDPI.

[6]  D. Annane,et al.  Selenocompounds and Sepsis: Redox Bypass Hypothesis: Part B- Selenocompounds in the Management of Early Sepsis. , 2022, Antioxidants & redox signaling.

[7]  Dariush Gholami,et al.  Supplementation of freezing medium with encapsulated or free glutathione during cryopreservation of bull sperm. , 2022, Reproduction in domestic animals = Zuchthygiene.

[8]  Gang Liu,et al.  Glutathione protects against the meiotic defects of ovine oocytes induced by arsenic exposure via the inhibition of mitochondrial dysfunctions. , 2021, Ecotoxicology and environmental safety.

[9]  S. Tiwari,et al.  Targeted antioxidant delivery modulates mitochondrial functions, ameliorates oxidative stress and preserve sperm quality during cryopreservation. , 2021, Theriogenology.

[10]  Gang Liu,et al.  Effect of exogenous glutathione supplementation on the in vitro developmental competence of ovine oocytes. , 2021, Theriogenology.

[11]  M. Fathi,et al.  Spirulina platensis extract addition to semen extender enhances cryotolerance and fertilizing potentials of buffalo bull spermatozoa , 2021, Animal reproduction.

[12]  V. Upadhyay,et al.  Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview , 2021, Andrologia.

[13]  K. Buranaamnuay.  The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols , 2021, Open veterinary journal.

[14]  A. El-Batal,et al.  Investigating the antimicrobial, antioxidant and cytotoxic activities of the biological synthesized glutathione selenium nano-incorporation , 2021, BioMetals.

[15]  F. Martínez-Pastor,et al.  Supplementation of the BIOXcell extender with the antioxidants crocin, curcumin and GSH for freezing bull semen. , 2021, Research in veterinary science.

[16]  S. Lee,et al.  The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation , 2020, Animals : an open access journal from MDPI.

[17]  R. Asadpour,et al.  Ameliorative effect of selenium nanoparticles on the structure and function of testis and in vitro embryo development in Aflatoxin B1‐exposed male mice , 2020, Andrologia.

[18]  X. Su,et al.  Effect of bioactive peptide on ram semen cryopreservation. , 2020, Cryobiology.

[19]  S. Dursun,et al.  Cryopreservation Effects on Ram Sperm Ultrastructure. , 2020, Biopreservation and biobanking.

[20]  V. Sahinturk,et al.  Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron. , 2020, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[21]  A. C. Jayasuriya,et al.  Recent trends in the application of widely used natural and synthetic polymer nanocomposites in bone tissue regeneration. , 2020, Materials science & engineering. C, Materials for biological applications.

[22]  A. Özcan,et al.  The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters. , 2020, Cryobiology.

[23]  S. Alijani,et al.  The effects of different levels of Nano selenium on the quality of frozen-thawed sperm in ram , 2020 .

[24]  M. Shahin,et al.  Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa , 2020, Animals : an open access journal from MDPI.

[25]  I. Çelik,et al.  Cryobiology and Cryopreservation of Sperm , 2019, Cryopreservation - Current Advances and Evaluations.

[26]  M. Sharafi,et al.  Effect of Glutathione Supplementation to Semen Extender on Post-thawed Rooster Sperm Quality Indices Frozen After Different Equilibration Times. , 2020, Cryo letters.

[27]  M. Sharafi,et al.  Supplementation of chilling storage medium with glutathione protects rooster sperm quality. , 2020, Cryobiology.

[28]  Prashant Kumar,et al.  Ameliorative Effect of Ascorbic Acid and Glutathione in Combating the Cryoinjuries During Cryopreservation of Exotic Jack Semen. , 2019, Journal of equine veterinary science.

[29]  Ho-Kyung Ha,et al.  Development and Characterization of Whey Protein-Based Nano-Delivery Systems: A Review , 2019, Molecules.

[30]  J. Rodríguez-Gil,et al.  Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semen. , 2019, Animal reproduction science.

[31]  Shi-Xiong Tan,et al.  The roles of reactive oxygen species and antioxidants in cryopreservation , 2019, Bioscience reports.

[32]  S. Lone,et al.  Sperm protein carbonylation , 2019, Andrologia.

[33]  M. Sharafi,et al.  Effects of reduced glutathione on the quality of rooster sperm during cryopreservation. , 2019, Theriogenology.

[34]  W. Khalil,et al.  Impact of selenium nano-particles in semen extender on bull sperm quality after cryopreservation. , 2019, Theriogenology.

[35]  A. Mishra,et al.  In Vitro Embryo Production in Sheep. , 2019, Methods in molecular biology.

[36]  Jing Zhang,et al.  Protective Effect of Sucrose and Antioxidants on Cryopreservation of Sperm Motility and DNA Integrity in C57BL/6 Mice. , 2018, Biopreservation and biobanking.

[37]  L. Aguila,et al.  Cryopreservation induces mitochondrial permeability transition in a bovine sperm model. , 2018, Cryobiology.

[38]  R. Biswas,et al.  Effect of Reduced Glutathione, Water Soluble Vitamin E Analogue and Butylated Hydroxytoluene on the Post Thaw Characteristics of Boar Spermatozoa. , 2018, Cryo letters.

[39]  J. Ravindra,et al.  Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet. , 2018, Cryobiology.

[40]  J. Riemer,et al.  Mitochondrial Glutathione: Regulation and Functions. , 2017, Antioxidants & redox signaling.

[41]  I. Z. Qureshi,et al.  Freezability of water buffalo bull (Bubalus bubalis) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender , 2017, Andrologia.

[42]  A. Saxena,et al.  Effect of reduced glutathione supplementation in semen extender on tyrosine phosphorylation and apoptosis like changes in frozen thawed Hariana bull spermatozoa. , 2017, Animal reproduction science.

[43]  G. Moghaddam,et al.  Effect of vitamin E and selenium nanoparticles on post-thaw variables and oxidative status of rooster semen. , 2016, Animal reproduction science.

[44]  S. Jahan,et al.  Semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season. , 2016, Theriogenology.

[45]  Muhammad Sohail Zafar,et al.  Modifications in Glass Ionomer Cements: Nano-Sized Fillers and Bioactive Nanoceramics , 2016, International journal of molecular sciences.

[46]  P. Santolaria,et al.  Effect of hypoosmotic swelling test and water test on the distribution of sperm subpopulations in bull , 2016 .

[47]  M. Nichi,et al.  Effect of reduced glutathione (GSH) in canine sperm cryopreservation: In vitro and in vivo evaluation. , 2016, Cryobiology.

[48]  F. Treulén,et al.  Mitochondrial outer membrane permeabilization increases reactive oxygen species production and decreases mean sperm velocity but is not associated with DNA fragmentation in human sperm. , 2016, Molecular human reproduction.

[49]  I. Amiri,et al.  PROTECTIVE EFFECTS OF GLUTATHIONE SUPPLEMENTATION AGAINST OXIDATIVE STRESS DURING CRYOPRESERVATION OF HUMAN SPERMATOZOA. , 2016, Cryo letters.

[50]  L. Anel-López,et al.  Reduced glutathione addition improves both the kinematics and physiological quality of post-thawed red deer sperm. , 2015, Animal reproduction science.

[51]  P. Lens,et al.  Selenium biomineralization for biotechnological applications. , 2015, Trends in biotechnology.

[52]  M. Yeste,et al.  Cryotolerance of stallion spermatozoa is related to ROS production and mitochondrial membrane potential rather than to the integrity of sperm nucleus , 2015, Andrology.

[53]  P. de Paz,et al.  Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. , 2015, Theriogenology.

[54]  M. Yeste,et al.  Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance. , 2015, Theriogenology.

[55]  Y. Nagao,et al.  Glutathione supplementation to semen extender improves the quality of frozen-thawed canine spermatozoa for transcervical insemination , 2015, The Journal of reproduction and development.

[56]  A. Shahverdi,et al.  Effect of cysteine and glutamine added to extender on post‐thaw sperm functional parameters of buffalo bull , 2014, Andrologia.

[57]  P. Loi,et al.  Impaired Placental Vasculogenesis Compromises the Growth of Sheep Embryos Developed In Vitro1 , 2014, Biology of reproduction.

[58]  P. B. Tuncer,et al.  Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability. , 2014, Cryobiology.

[59]  I. Ashrafi,et al.  Effects of in combination antioxidant supplementation on microscopic and oxidative parameters of freeze–thaw bull sperm , 2014, Cell and Tissue Banking.

[60]  J. Gadea,et al.  Supplementation of the thawing medium with reduced glutathione improves function of frozen-thawed goat spermatozoa. , 2013, Reproductive biology.

[61]  M. Abdollahi,et al.  Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. , 2013, Toxicology and applied pharmacology.

[62]  W. Holt,et al.  Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. , 2012, Reproductive biology.

[63]  A. D. de Andrade,et al.  Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa , 2011, Journal of veterinary science.

[64]  P. Lonergan,et al.  In vitro assessment of sperm from bulls of high and low field fertility. , 2011, Theriogenology.

[65]  P. B. Tuncer,et al.  Effect of glutamine and sugars after bull spermatozoa cryopreservation. , 2011, Theriogenology.

[66]  Huiping Yang,et al.  High-throughput cryopreservation of spermatozoa of blue catfish (Ictalurus furcatus): Establishment of an approach for commercial-scale processing. , 2011, Cryobiology.

[67]  D. Oztuna,et al.  Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage. , 2010, Cryobiology.

[68]  A. F. Fidan,et al.  The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen. , 2010, Cryobiology.

[69]  A. Shirazi,et al.  Effects of growth hormone on nuclear maturation of ovine oocytes and subsequent embryo development. , 2008, Reproduction in domestic animals = Zuchthygiene.

[70]  F. Edens,et al.  Sel-Plex® improves spermatozoa morphology in broiler breeder males. , 2009 .

[71]  M. Anton,et al.  Effect of glutamine on post-thaw motility of bull spermatozoa after association with LDL (low density lipoproteins) extender: preliminary results. , 2009, Theriogenology.

[72]  Hongqiao Zhang,et al.  Glutathione: overview of its protective roles, measurement, and biosynthesis. , 2009, Molecular aspects of medicine.

[73]  M. Zamiri,et al.  Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender. , 2008, Animal reproduction science.

[74]  N. Tekin,et al.  Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen , 2007 .

[75]  Bucak Mustafa Numan,et al.  Kryoprotektanlar ve gamet hücrelerinin dondurulmasında kryoprotektif etki , 2007 .

[76]  D. Aziz.  Assessment of bovine sperm viability by MTT reduction assay. , 2006, Animal reproduction science.

[77]  J. Gadea,et al.  Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation. , 2005, Journal of andrology.

[78]  D. Aziz,et al.  Application of MTT reduction assay to evaluate equine sperm viability. , 2005, Theriogenology.

[79]  F. García-Vázquez,et al.  Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. , 2005, Journal of andrology.

[80]  R. Foote,et al.  Motility and fertility of bull sperm in whole milk extender containing antioxidants. , 2002, Animal reproduction science.

[81]  Y. Takahashi,et al.  Effects of glutamine, glycine and taurine on the development of in vitro fertilized bovine zygotes in a chemically defined medium. , 1998, The Journal of veterinary medical science.

[82]  T. Vanha-Perttula,et al.  Semen selenium content and sperm mitochondrial volume in human and some animal species. , 1989, Human reproduction.