Optical trapping dynamics for cell identification

Living cells show a variety of morphological traits upon which numerous identification techniques have already been developed. However most of them involve lengthy biochemical procedures and can compromise the viability of the cell. We demonstrate a method to differentiate cells only on the basis of its trapping dynamics while it is being drawn into an optical trap (Optical Trapping Dynamics). Since it relies only on the inherent properties of the optical trap, without requiring external markers or biochemically sensitive spectroscopic techniques, it can be readily combined with existing optical tweezers setups. We applied it to the study of the yeast cell-cycle stages, showing, in particular, how it can be amenable for the measurement of the budding index of a cell population.

[1]  Stefan Schinkinger,et al.  Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. , 2005, Biophysical journal.

[2]  K. Neuman,et al.  Optical trapping. , 2004, The Review of scientific instruments.

[3]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .

[4]  Peter John Rodrigo,et al.  Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture. , 2005, FEMS microbiology letters.

[5]  Keiichi Torimitsu,et al.  Laser trapping and Raman spectroscopy of single cellular organelles in the nanometer range. , 2002, Lab on a chip.

[6]  Giovanni Volpe,et al.  Dynamics of a growing cell in an optical trap , 2006 .

[7]  G. Shivashankar,et al.  Single particle tracking of correlated bacterial dynamics. , 2003, Biophysical journal.

[8]  D. Grier.  A revolution in optical manipulation , 2003, Nature.

[9]  E. Riis,et al.  Laser cooling and trapping of neutral atoms , 1997 .

[10]  Giovanni Volpe,et al.  The lag phase and G1 phase of a single yeast cell monitored by Raman microspectroscopy , 2006 .

[11]  Kim R. Rogers,et al.  Biosensors for environmental applications , 1995 .

[12]  L. Novotný,et al.  Detection of nanoparticles using optical gradient forces , 2003 .

[13]  H. Metcalf,et al.  Laser Cooling and Trapping of Neutral Atoms , 2004 .

[14]  R M Doornbos,et al.  Elastic light-scattering measurements of single biological cells in an optical trap. , 1996, Applied optics.

[15]  Jochen Arlt,et al.  Measuring fungal growth forces with optical tweezers , 2005, SPIE Optics + Photonics.

[16]  P. F. Mullaney,et al.  Differential light scattering from spherical mammalian cells. , 1974, Biophysical journal.

[17]  Watt W. Webb,et al.  Measurement of small forces using an optical trap , 1994 .

[18]  Giovanni Volpe,et al.  Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy. , 2005, Analytical chemistry.

[19]  Stefan Seeger,et al.  Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers , 1999, FEBS letters.

[20]  Wolfgang Singer,et al.  Three-dimensional force calibration of optical tweezers , 2000 .

[21]  Dmitri Ivnitski,et al.  Nucleic acid approaches for detection and identification of biological warfare and infectious disease agents. , 2003, BioTechniques.

[22]  P. Wyatt.  Identification of Bacteria by Differential Light Scattering , 1969, Nature.

[23]  H. Flyvbjerg,et al.  Power spectrum analysis for optical tweezers , 2004 .

[24]  H. V. Hulst.  Light Scattering by Small Particles , 1957 .

[25]  T. Allen,et al.  Precision of light scattering techniques for measuring optical parameters of microspheres. , 1991, Applied optics.

[26]  A. Mehta,et al.  Single-molecule biomechanics with optical methods. , 1999, Science.

[27]  Deepak Mathur,et al.  Torque-generating malaria-infected red blood cells in an optical trap. , 2004, Optics express.

[28]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[29]  E. Stelzer,et al.  Three-dimensional position detection of optically trapped dielectric particles , 2002 .

[30]  G. Cooper.  The Cell: A Molecular Approach , 1996 .

[31]  Michael F. Zettel,et al.  The budding index of Saccharomyces cerevisiae deletion strains identifies genes important for cell cycle progression. , 2003, FEMS microbiology letters.

[32]  Yong-qing Li,et al.  Near-infrared Raman spectroscopy of single optically trapped biological cells. , 2002, Optics letters.

[33]  Khyati Mohanty,et al.  Dynamics of Interaction of RBC with optical tweezers. , 2005, Optics express.

[34]  A. Givan,et al.  Flow Cytometry: First Principles , 1992 .

[35]  Kishan Dholakia,et al.  Light-induced cell separation in a tailored optical landscape , 2005 .