Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics

Biodegradation process modeling is an essential tool for the optimization of biotechnologies related to gaseous pollutant treatment. In these technologies, the predominant role of biofilm, particularly under conditions of no mass transfer limitations, results in a need to determine what processes are occurring within the same. By measuring the interior of the biofilms, an increased knowledge of mass transport and biodegradation processes may be attained. This information is useful in order to develop more reliable models that take biofilm heterogeneity into account. In this study, a new methodology, based on a novel dissolved oxygen (DO) and mass transport microelectronic array (MEA) sensor, is presented in order to characterize a biofilm. Utilizing the MEA sensor, designed to obtain DO and diffusivity profiles with a single measurement, it was possible to obtain distributions of oxygen diffusivity and biokinetic parameters along a biofilm grown in a flat plate bioreactor (FPB). The results obtained for oxygen diffusivity, estimated from oxygenation profiles and direct measurements, revealed that changes in its distribution were reduced when increasing the liquid flow rate. It was also possible to observe the effect of biofilm heterogeneity through biokinetic parameters, estimated using the DO profiles. Biokinetic parameters, including maximum specific growth rate, the Monod half-saturation coefficient of oxygen, and the maintenance coefficient for oxygen which showed a marked variation across the biofilm, suggest that a tool that considers the heterogeneity of biofilms is essential for the optimization of biotechnologies.

[1]  M. Suidan,et al.  Biofilm structure and mass transfer in a gas phase trickle-bed biofilter. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.

[2]  Juan A. Baeza,et al.  Biomass accumulation in a biofilter treating toluene at high loads – Part 1: Experimental performance from inoculation to clogging , 2012 .

[3]  Tian C. Zhang,et al.  Determination of effective oxygen diffusivity in biofilms grown in a completely mixed biodrum reactor , 1994 .

[4]  Z. Lewandowski,et al.  Quantifying selected growth parameters of Leptothrix discophora SP-6 in biofilms from oxygen concentration profiles , 2003 .

[5]  S. Okabe,et al.  Analyses of Spatial Distributions of Sulfate-Reducing Bacteria and Their Activity in Aerobic Wastewater Biofilms , 1999, Applied and Environmental Microbiology.

[6]  Jeonghee Lee,et al.  Natural convection at microelectrodes , 1995 .

[7]  M. Duch,et al.  Microfabrication and characterization of cylinder micropillar array electrodes , 2011 .

[8]  J J Heijnen,et al.  Mathematical modeling of biofilm structure with a hybrid differential-discrete cellular automaton approach. , 1998, Biotechnology and bioengineering.

[9]  A. Baldi,et al.  Electrical readout of protein microarrays on regular glass slides. , 2011, Analytical chemistry.

[10]  Francesc Xavier Muñoz,et al.  Continuous measurement of acute toxicity in water using a solid state microrespirometer , 2007 .

[11]  Tian C. Zhang,et al.  Density, porosity, and pore structure of biofilms , 1994 .

[12]  Tomoyuki Yasukawa,et al.  Fabrication of miniature Clark oxygen sensor integrated with microstructure , 2005 .

[13]  J. C. van den Heuvel,et al.  Diffusion coefficients of metabolites in active biofilms. , 2000, Biotechnology and bioengineering.

[14]  Z. Lewandowski,et al.  Internal and External Mass Transfer in Biofilms Grown at Various Flow Velocities , 2002, Biotechnology progress.

[15]  Peter Berg,et al.  Interpretation of measured concentration profiles in sediment pore water , 1998 .

[16]  B. Jørgensen,et al.  Microelectrodes: Their Use in Microbial Ecology , 1986 .

[17]  Anja Boisen,et al.  Gold cleaning methods for electrochemical detection applications , 2009 .

[18]  Don W. Green,et al.  Perry's chemical engineers' handbook. 7th ed. , 1997 .

[19]  J. Tay,et al.  Diffusivity of oxygen in aerobic granules , 2006, Biotechnology and bioengineering.

[20]  Z. Lewandowski,et al.  Microelectrode measurements of local mass transport rates in heterogeneous biofilms. , 1998, Biotechnology and bioengineering.

[21]  K. Schleifer,et al.  Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes , 1996, Applied and environmental microbiology.

[22]  R. Villa,et al.  Flexible probe for in vivo quantification of corneal epithelium permeability through non-invasive tetrapolar impedance measurements , 2013, Biomedical Microdevices.

[23]  Haluk Beyenal,et al.  Fundamentals of biofilm research , 2007 .

[24]  Tae-Sun Lim,et al.  Needle-type dissolved oxygen microelectrode array sensors for in situ measurements , 2007 .

[25]  L. Nielsen,et al.  A novel microsensor for determination of apparent diffusivity in sediments , 1998 .

[26]  Don W. Green,et al.  Perry's Chemical Engineers' Handbook , 2007 .

[27]  Han-Qing Yu,et al.  Measurement of dissolved oxygen and its diffusivity in aerobic granules using a lithographically-fabricated microelectrode array. , 2009, Environmental science & technology.

[28]  M. Deshusses,et al.  Development and experimental validation of a conceptual model for biotrickling filtration of H2S , 2003 .

[29]  Haluk Beyenal,et al.  Measurement of local effective diffusivity in heterogeneous biofilms , 1998 .

[30]  L. Melo.  Biofilm physical structure, internal diffusivity and tortuosity , 2005 .

[31]  G. Gabriel,et al.  A non-invasive method for an in vivo assessment of corneal epithelium permeability through tetrapolar impedance measurements. , 2012, Biosensors & bioelectronics.

[32]  Horacio A. Mottola,et al.  Enzymic substrate determination in closed flow-through systems by sample injection and amperometric monitoring of dissolved oxygen levels , 1978 .

[33]  D. Hempel,et al.  Effective diffusivities and mass fluxes in fungal biopellets , 2009, Biotechnology and bioengineering.

[34]  Haluk Beyenal,et al.  Combined effect of substrate concentration and flow velocity on effective diffusivity in biofilms , 2000 .

[35]  Carlos Paliteiro,et al.  (100)-Type behaviour of polycrystalline gold towards O2 reduction , 1994 .

[36]  David A. Mitchell,et al.  A review of recent developments in modeling of microbial growth kinetics and intraparticle phenomena in solid-state fermentation , 2004 .

[37]  A. Gieseke,et al.  Impact of Nitrate on the Structure and Function of Bacterial Biofilm Communities in Pipelines Used for Injection of Seawater into Oil Fields , 2008, Applied and Environmental Microbiology.

[38]  Xavier Borrisé,et al.  Determination of heterogeneous electron transfer rate constants at interdigitated nanoband electrodes fabricated by an optical mix-and-match process , 2014 .

[39]  Rosa Villa,et al.  Manufacturing and full characterization of silicon carbide-based multi-sensor micro-probes for biomedical applications , 2007, Microelectron. J..

[40]  Tong Yu,et al.  Development of an automation system to evaluate the three-dimensional oxygen distribution in wastewater biofilms using microsensors , 2006 .

[41]  Xiaohong Zhou,et al.  Estimation of Heterotrophic Biokinetic Parameters in Wastewater Biofilms from Oxygen Concentration Profiles by Microelectrode , 2012 .

[42]  D. A. Dawson,et al.  Mass transfer at rough surfaces , 1972 .

[43]  Multiple Substrate Growth Kinetics of Leptothrix discophoraSP‐6 , 2002, Biotechnology progress.

[44]  D. Dávila,et al.  Measuring acute toxicity using a solid-state microrespirometer: Part II. A theoretical framework for the elucidation of metabolic parameters , 2008 .

[45]  Z Lewandowski,et al.  Measurement of local mass transfer coefficient in biofilms , 1995, Biotechnology and bioengineering.

[46]  F. Fang,et al.  Development of an in situ dissolved oxygen measurement system and calculation of its effective diffusion coefficient in a biofilm , 2012 .

[47]  J. Maestre,et al.  Modeling of a bacterial and fungal biofilter applied to toluene abatement: kinetic parameters estimation and model validation , 2008 .

[48]  Akira Hirata,et al.  Simple prediction of oxygen penetration depth in biofilms for wastewater treatment , 2004 .

[49]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[50]  Tian C. Zhang,et al.  Effects of biofilm structure, microbial distributions and mass transport on biodegradation processes , 1995 .

[51]  Karel J. Keesman,et al.  Identification of activated sludge and wastewater characteristics using respirometric batch-experiments , 1998 .