Peroxyoxalate chemiluminescence detection of polycyclic aromatic hydrocarbons in liquid chromatography

Peroxyoxalate chemiluminescence is applied to the high-performance liquid chromatographic detection of polycyclic aromatic hydrocarbons (PAH). PAH are excited by energy transfer from the decomposition products of the reaction between hydrogen peroxide and bis(2,4,6-trichlorophenyl) oxalate. These reagents are introduced by postcolumn mixing, and the emission is observed by using a conventional fluorescence detector with its source turned off. The method yields linear responses to PAH over 3 orders of magnitude, and in some cases detection limits are better than those determined by fluorescence using the same fluorometer. Chemiluminescence detection is compared to ultraviolet absorbance and fluorescence detection of PAH in terms of sensitivity and selectivity.

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