Noninvolvement of the X chromosome in radiation-induced chromosome translocations in the human lymphoblastoid cell line TK6.

Fluorescence in situ hybridization procedures were used to examine the influence of chromosome locus on the frequency and type of chromosome aberrations induced by 60Co gamma rays in the human lymphoblastoid cell line TK6. Aberrations involving the X chromosome were compared to those involving the similarly sized autosome chromosome 7. When corrected for DNA content, acentric fragments were induced with equal frequency in the X and 7 chromosomes. Dose-dependent increases in chromosomal interchanges involving chromosome 7 were noted and the frequencies of balanced translocations and dicentrics produced were approximately equal. Chromosome interchanges involving the X chromosome were rare and showed no apparent dose dependence. Thus, while chromosomes 7 and X are equally sensitive to the induction of chromosome breaks, the X chromosome is much less likely to interact with autosomes than chromosome 7. The noninvolvement of the X chromosome in translocations with autosomes may reflect a more peripheral and separate location for the X chromosome in the mammalian nucleus.

[1]  P. Olive,et al.  DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines. , 1993, Radiation research.

[2]  D. Pinkel,et al.  Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation. , 1992, International journal of radiation biology.

[3]  J. Gray,et al.  Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes. , 1992, International journal of radiation biology.

[4]  A. Natarajan,et al.  Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries. , 1992, International journal of radiation biology.

[5]  J. Schwartz,et al.  Differential locus sensitivity to mutation induction by ionizing radiations of different LETs in Chinese hamster ovary K1 cells. , 1991, Carcinogenesis.

[6]  P. Bryant,et al.  Enhanced mutability at the tk locus in the radiosensitive double-strand break repair mutant xrs5. , 1990, Mutation research.

[7]  H. Liber,et al.  A comparison of mutation induction at the tk and hprt loci in human lymphoblastoid cells; quantitative differences are due to an additional class of mutations at the autosomal tk locus. , 1989, Mutation research.

[8]  F. D. de Serres,et al.  Specific-locus mutations induced in eukaryotes (especially mammalian cells) by radiation and chemicals: a perspective. , 1989, Mutation research.

[9]  H. H. Evans,et al.  Locus specificity in the mutability of L5178Y mouse lymphoma cells: the role of multilocus lesions. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J W Gray,et al.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Little,et al.  Chromosome 14 marker appearance in a human B lymphoblastoid cell line of nonmalignant origin. , 1986, Cancer genetics and cytogenetics.

[12]  D. Clive,et al.  Analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/- mouse lymphoma cells. , 1985, Mutation research.

[13]  R. Weichselbaum,et al.  Similar sensitivity of human lymphoblast cells proficient or deficient in nucleotide biosynthesis to the cytotoxic and cell cycle effects of 3-aminobenzamide. , 1985, Experimental cell research.

[14]  B H Mayall,et al.  The DNA-based human karyotype. , 1984, Cytometry.

[15]  T. Skopek,et al.  Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. , 1978, Biochemical and biophysical research communications.

[16]  M. Seabright.  Noninvolvement of the human X chromosome in X-ray induced exchanges. , 1973, Cytogenetics and cell genetics.

[17]  C. E. Ford,et al.  Studies on the induction of translocations in mouse spermatogonia. IV. Effects of acute gamma-irradiation. , 1971, Mutation research.

[18]  R F Potthoff,et al.  Testing for homogeneity. II. The Poisson distribution. , 1966, Biometrika.