QUANTITATIVE ESTIMATION OF DIATOM ALGAE USING THE SCANNING AND TRANSMISSION ELECTRON MICROSCOPES

A method is proposed for the quantitative assessment of phytoplankton using electron microscopy. It has been tested on phytoplankton samples containing diatom populations from thousands to millions of cells per litre. The results of diatom counts using SEM and TEM appear to be quite comparable with data obtained using the light microscope. The method may be used for studies of the nannoplankton fraction (Bacillariophyta) of the phytoplankton.