A Smart Autocatalytic DNAzyme Biocircuit for in vivo Amplified MicroRNA Imaging.

DNAzymes have been recognized as promising transducing agents for visualizing endogenous biomarkers, while their inefficient intracellular delivery (including blunt responsibility) and limited amplification capacity (including insufficient cofactor supply) preclude their extensive biological applications. Here an exquisite autocatalytic DNAzyme (ACD) biocircuit is constructed for in vivo amplified microRNA imaging based on honeycomb MnO 2 nanosponge (hMNS)-sustained hybridization chain reaction (HCR) and DNAzyme biocatalysis. The versatile hMNS scaffolds not only deliver DNA probes, but also supply appropriate Mn 2+ -DNAzyme cofactors and intelligent magnetic resonance imaging (MRI) agents into cancer cells. Through the subsequent synergistic cross-activation between HCR and DNAzyme amplicons, the ACD amplifier turns the limited miRNA-recognition into tremendously amplified readout, thus contributing to the accurate tumor diagnosis. As a robust sensing strategy, the intelligent autocatalytic amplifier realized the microRNA imaging in vivo, thus showing great promise in clinical theranostic.

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