Detection of a tospovirus infecting Phalaenopsis amabilis in Yunnan

Using electron microscopy,DAS-ELISA and RT-PCR,a virus isolate(Tospo-Pha)was detected from Phalaenopsis amabilis showing yellow ringspot symptoms in Yunnan.Tospo-Pha had quasi-spherical,enveloped shape with diameters of 90 nm,reacted positively to compound antibody of Watermelon silver mosaic virus(WSMoV)/Groundnut bud necrosis virus(GBNV).The S RNA 5'-termini sequence of Tospo-Pha had highest identity(91.0 %)with that of CaCV isolate Gloxinia(CaCV-Gloxinia).The Tospo-Pha clustered with other CaCV isolates in phylogenetic tree.These results indicated Tospo-Pha belonged to the genus Tospovirus.