Surface Acoustic Wave Sensor for C-Reactive Protein Detection

A surface acoustic wave (SAW) sensor was investigated for its application in C-reactive protein (CRP) detection. Piezoelectric lithium niobate (LiNbO3) substrates were used to study their frequency response characteristics in a SAW sensor with a CRP sensing area. After the fabrication of the SAW sensor, the immobilization process was performed for CRP/anti-CRP interaction. The CRP/anti-CRP interaction can be detected as mass variations in the sensing area. These mass variations may produce changes in the amplitude of sensor response. It was clearly observed that a CRP concentration of 0.1 μg/mL can be detected in the proposed SAW sensor. A good fitting linear relationship between the detected insertion loss (amplitude) and the concentrations of CRP from 0.1 μg/mL to 1 mg/mL was obtained. The detected shifts in the amplitude of insertion loss in SAW sensors for different CRP concentrations may be useful in the diagnosis of risk of cardiovascular diseases.

[1]  Pablo García,et al.  Love Wave Immunosensor for the Detection of Carbaryl Pesticide , 2014, Sensors.

[2]  K. Hashimoto.  Surface Acoustic Wave Devices in Telecommunications , 2000 .

[3]  Xinran Hu,et al.  Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer , 2015, PloS one.

[4]  Liesbet Lagae,et al.  Impact of spacers on the hybridization efficiency of mixed self-assembled DNA/alkanethiol films. , 2008, Biosensors & bioelectronics.

[5]  Xu Bing,et al.  Label Free C-reactive Protein Detection Based on An Electrochemical Sensor for Clinical Application , 2017 .

[6]  J. Stuart,et al.  C reactive protein rapid assay techniques for monitoring resolution of infection in immunosuppressed patients. , 1984, Journal of clinical pathology.

[7]  Stephen J. Martin,et al.  Dynamics and Response of Polymer-Coated Surface Acoustic Wave Devices: Effect of Viscoelastic Properties and Film Resonance , 1994 .

[8]  Tao Wang,et al.  Surface acoustic wave based pumping in a microchannel , 2017 .

[9]  Fei Xu,et al.  A novel quantum dot-based fluoroimmunoassay method for detection of Enrofloxacin residue in chicken muscle tissue , 2009 .

[10]  M. Sandros,et al.  Zeptomole Detection of C-Reactive Protein in Serum by a Nanoparticle Amplified Surface Plasmon Resonance Imaging Aptasensor , 2014, Scientific Reports.

[11]  T. D. Du Clos.  Function of C-reactive protein , 2000, Annals of medicine.

[12]  R. Cingolani,et al.  Noise reduction in GaN-based radio frequencysurface acoustic wave filters , 2004 .

[13]  S. Saeger,et al.  Quantum dot based rapid tests for zearalenone detection , 2012, Analytical and Bioanalytical Chemistry.

[14]  S Büttgenbach,et al.  Properties of sensors based on shear-horizontal surface acoustic waves in LiTaO3/SiO2 and quartz/SiO2 structures. , 2001, IEEE transactions on ultrasonics, ferroelectrics, and frequency control.

[15]  Chuin-Shan Chen,et al.  Electrical Detection of C-Reactive Protein Using a Single Free-Standing, Thermally Controlled Piezoresistive Microcantilever for Highly Reproducible and Accurate Measurements , 2013, Sensors.

[16]  Jing Ma,et al.  Inflammatory markers and the risk of coronary heart disease in men and women. , 2004, The New England journal of medicine.

[17]  Rasim Guldiken,et al.  Surface Modification on Acoustic Wave Biosensors for Enhanced Specificity , 2012, Sensors.

[18]  H. M. Gerard,et al.  Design of Surface Wave Delay Lines with Interdigital Transducers , 1969 .

[19]  P. Macfarlane,et al.  Metabolic Syndrome With and Without C-Reactive Protein as a Predictor of Coronary Heart Disease and Diabetes in the West of Scotland Coronary Prevention Study , 2003, Circulation.

[20]  W. Wlodarski,et al.  A layered SAW device based on ZnO/LiTaO/sub 3/ for liquid media sensing applications , 2002, 2002 IEEE Ultrasonics Symposium, 2002. Proceedings..

[21]  Pietro Salvo,et al.  Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review , 2017, Sensors.

[22]  S. Buttgenbach,et al.  Properties of sensors based on shear-horizontal surface acoustic waves in LiTaO/sub 3//SiO/sub 2/ and quartz/SiO/sub 2/ structures , 2001, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[23]  I. Ruge,et al.  Acoustic sensors based on surface-localized HPSWs for measurements in liquids , 1993 .

[24]  Andrew J. Shields,et al.  Modulation of single quantum dot energy levels by a surface-acoustic-wave , 2008 .

[25]  Rasim Guldiken,et al.  A Urinary Bcl-2 Surface Acoustic Wave Biosensor for Early Ovarian Cancer Detection , 2012, Sensors.

[26]  C. Santschi,et al.  Label-Free Electrochemical Immunoassay for C-Reactive Protein , 2018, Biosensors.

[27]  Bruce M Psaty,et al.  Inflammation as a Risk Factor for Atrial Fibrillation , 2003, Circulation.

[28]  Gina Greco,et al.  A Rayleigh surface acoustic wave (R-SAW) resonator biosensor based on positive and negative reflectors with sub-nanomolar limit of detection , 2018 .

[29]  Hsueh-Chia Chang,et al.  Surface acoustic wave devices for chemical sensing and microfluidics: A review and perspective. , 2017, Analytical methods : advancing methods and applications.

[30]  D. Dosdall,et al.  Perioperative Biomarkers Predicting Postoperative Atrial Fibrillation Risk After Coronary Artery Bypass Grafting: A Narrative Review. , 2020, Journal of cardiothoracic and vascular anesthesia.

[31]  Bastian E. Rapp,et al.  Surface acoustic wave biosensors: a review , 2008, Analytical and bioanalytical chemistry.

[32]  Kazuhiko Yamanouchi,et al.  Field analysis o SAW single-phase unidirectional transducers using internal floating electrodes , 1988, IEEE 1988 Ultrasonics Symposium Proceedings..

[33]  Konstantinos Mitsakakis,et al.  Detection of multiple cardiac markers with an integrated acoustic platform for cardiovascular risk assessment. , 2011, Analytica chimica acta.

[34]  C. Lowe,et al.  A novel Love-plate acoustic sensor utilizing polymer overlayers , 1992, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[35]  Dong-Kyung Kim,et al.  Development of indirect-competitive quartz crystal microbalance immunosensor for C-reactive protein , 2009 .

[36]  E. Engvall,et al.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. , 1971, Immunochemistry.

[37]  A. Gautier,et al.  C-reactive protein , 2005 .

[38]  T. Aastrup,et al.  Signal enhancement in ligand-receptor interactions using dynamic polymers at quartz crystal microbalance sensors. , 2016, The Analyst.

[39]  S. K. Vashist,et al.  Surface plasmon resonance-based immunoassay for human C-reactive protein. , 2015, The Analyst.

[40]  Yuzhi Chen,et al.  A label-free fiber optic SPR biosensor for specific detection of C-reactive protein , 2017, Scientific Reports.

[41]  S. Büttgenbach,et al.  Separate determination of liquid density and viscosity with sagittally corrugated Love-mode sensors , 1999 .

[42]  Chuan Li,et al.  The Characterization of Surface Acoustic Wave Devices Based on AlN-Metal Structures , 2016, Sensors.

[43]  L. Lagae,et al.  Improving the selectivity by using different blocking agents in DNA hybridization assays for SiGe bio-molecular sensors , 2013 .

[44]  R. Aigner,et al.  SAW and BAW technologies for RF filter applications: A review of the relative strengths and weaknesses , 2008, 2008 IEEE Ultrasonics Symposium.

[45]  Santosh K. Misra,et al.  Electrochemical-digital immunosensor with enhanced sensitivity for detecting human salivary glucocorticoid hormone. , 2018, The Analyst.