Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

[1]  Zhenghong Xu,et al.  Fungal His-Tagged Nitrilase from Gibberella intermedia: Gene Cloning, Heterologous Expression and Biochemical Properties , 2012, PloS one.

[2]  R. Chaloupkova,et al.  Purification and characterization of nitrilase from Fusarium solani IMI196840 , 2010 .

[3]  David Kubáč,et al.  Fungal nitrilases as biocatalysts: Recent developments. , 2009, Biotechnology advances.

[4]  L. Wackett,et al.  Investigative mining of sequence data for novel enzymes: a case study with nitrilases. , 2009, Journal of biotechnology.

[5]  M. Cantarella,et al.  Continuous hydrolysis of 4-cyanopyridine by nitrilases from Fusarium solani O1 and Aspergillus niger K10 , 2009, Applied Microbiology and Biotechnology.

[6]  F. Ahmad,et al.  Glycine betaine may have opposite effects on protein stability at high and low pH values. , 2009, Biochimica et biophysica acta.

[7]  B. Sewell,et al.  The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat , 2009, Applied Microbiology and Biotechnology.

[8]  M. Jeya,et al.  Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5 , 2009, Applied Microbiology and Biotechnology.

[9]  R. Stevens,et al.  Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes. , 2008, Biochemistry.

[10]  Richard C. Willson,et al.  Genome mining of cyanide-degrading nitrilases from filamentous fungi , 2008, Applied Microbiology and Biotechnology.

[11]  Dong-Eun Kim,et al.  An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33278 determines the substrate specificity for aliphatic and aromatic nitriles , 2008, The Biochemical journal.

[12]  J. Piškur,et al.  The crystal structure of beta-alanine synthase from Drosophila melanogaster reveals a homooctameric helical turn-like assembly. , 2008, Journal of molecular biology.

[13]  O. Benada,et al.  Purification and characterization of a nitrilase from Fusarium solani O1 , 2008 .

[14]  B. Sewell,et al.  Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi. , 2008, Journal of structural biology.

[15]  Dunming Zhu,et al.  A new nitrilase from Bradyrhizobium japonicum USDA 110. Gene cloning, biochemical characterization and substrate specificity. , 2008, Journal of biotechnology.

[16]  A. Stolz,et al.  Influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from Pseudomonas fluorescens EBC191. , 2007, Protein engineering, design & selection : PEDS.

[17]  M. Payne,et al.  Protein engineering of Acidovorax facilis 72W nitrilase for bioprocess development , 2007, Biotechnology and bioengineering.

[18]  Manfred J. Sippl,et al.  Thirty years of environmental health research--and growing. , 1996, Nucleic Acids Res..

[19]  B Trevor Sewell,et al.  Post‐translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form , 2007, The FEBS journal.

[20]  M. Mackova,et al.  Purification and characterization of a nitrilase from Aspergillus niger K10 , 2006, Applied Microbiology and Biotechnology.

[21]  C. Vorgias,et al.  Cloning, overexpression, and characterization of a thermoactive nitrilase from the hyperthermophilic archaeon Pyrococcus abyssi. , 2006, Protein expression and purification.

[22]  B. Sewell,et al.  Oligomeric Structure of Nitrilases: Effect of Mutating Interfacial Residues on Activity , 2005, Annals of the New York Academy of Sciences.

[23]  Joachim Klein,et al.  Nitrilase from Pseudomonas fluorescens EBC191: cloning and heterologous expression of the gene and biochemical characterization of the recombinant enzyme. , 2005, Microbiology.

[24]  Mircea Podar,et al.  Evolution of a microbial nitrilase gene family: a comparative and environmental genomics study , 2005, BMC Evolutionary Biology.

[25]  Gert Vriend,et al.  Making optimal use of empirical energy functions: Force‐field parameterization in crystal space , 2004, Proteins.

[26]  I. Tanaka,et al.  Crystal structure of hypothetical protein PH0642 from Pyrococcus horikoshii at 1.6Å resolution , 2004, Proteins.

[27]  P. Turner,et al.  The nitrilase family of CN hydrolysing enzymes – a comparative study , 2003, Journal of applied microbiology.

[28]  P. Schuck On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation. , 2003, Analytical biochemistry.

[29]  Andreas Stolz,et al.  Cloning of a Nitrilase Gene from the Cyanobacterium Synechocystis sp. Strain PCC6803 and Heterologous Expression and Characterization of the Encoded Protein , 2003, Applied and Environmental Microbiology.

[30]  F. Studier,et al.  Crystal structure of a putative CN hydrolase from yeast , 2003, Proteins.

[31]  Richard C. Willson,et al.  CynD, the Cyanide Dihydratase from Bacillus pumilus: Gene Cloning and Structural Studies , 2003, Applied and Environmental Microbiology.

[32]  R. Vohra,et al.  Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. , 2001, Protein expression and purification.

[33]  D. Higgins,et al.  T-Coffee: A novel method for fast and accurate multiple sequence alignment. , 2000, Journal of molecular biology.

[34]  Y. Pekarsky,et al.  Crystal structure of the worm NitFhit Rosetta Stone protein reveals a Nit tetramer binding two Fhit dimers , 2000, Current Biology.

[35]  P. Schuck,et al.  Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. , 2000, Biophysical journal.

[36]  D. Cowan,et al.  Characterization of an inducible nitrilase from a thermophilic bacillus , 1999, Extremophiles.

[37]  A. Willetts,et al.  Characterization and partial purification of an enantioselective arylacetonitrilase from Pseudomonas fluorescens DSM 7155 , 1998 .

[38]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[39]  E. Mandelkow,et al.  Crystallization and preliminary X-ray analysis of the single-headed and double-headed motor protein kinesin. , 1997, Journal of structural biology.

[40]  David S. Goodsell,et al.  Distributed automated docking of flexible ligands to proteins: Parallel applications of AutoDock 2.4 , 1996, J. Comput. Aided Mol. Des..

[41]  T. Mase,et al.  Purification and characterization of a new lipase from Fusarium sp. YM-30. , 1995, Bioscience, biotechnology, and biochemistry.

[42]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[43]  E. Weiler,et al.  Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: key enzymes in biosynthesis of the plant hormone indole-3-acetic acid. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[44]  T. Blundell,et al.  Comparative protein modelling by satisfaction of spatial restraints. , 1993, Journal of molecular biology.

[45]  J. Thornton,et al.  PROCHECK: a program to check the stereochemical quality of protein structures , 1993 .

[46]  H. Yamada,et al.  Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residue. , 1992, Biochemistry.

[47]  H. Vanetten,et al.  Cloning and properties of a cyanide hydratase gene from the phytopathogenic fungus Gloeocercospora sorghi. , 1992, Biochemical and biophysical research communications.

[48]  D. W. Bolen,et al.  Increased thermal stability of proteins in the presence of naturally occurring osmolytes. , 1992, Biochemistry.

[49]  O. Benada,et al.  Modification of the Polaron sputter-coater unit for glow-discharge activation of carbon support films. , 1990, Journal of electron microscopy technique.

[50]  H. Yamada,et al.  Nitrilase of Rhodococcus rhodochrous J1 , 1989 .

[51]  D. B. Harper,et al.  Fungal degradation of aromatic nitriles. Enzymology of C-N cleavage by Fusarium solani. , 1977, The Biochemical journal.

[52]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[53]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[54]  A. Banerjee,et al.  Enantioselective Nitrilase from Pseudomonas putida: Cloning, Heterologous Expression, and Bioreactor Studies , 2009, Molecular biotechnology.

[55]  H. Wajant,et al.  Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana. , 2002, European journal of biochemistry.

[56]  A. Goldlust,et al.  Induction, purification, and characterization of the nitrilase of Fusarium oxysporum f. sp. melonis , 1989 .