Using direct immunofluorescence to detect coronaviruses in peritoneal in peritoneal and pleural effusions

ABSTRACT Twenty‐one cases of feline infectious peritonitis (FIP) were diagnosed using a direct immunofluorescence test on cytocentrifuged pleural and peritoneal effusions from cats sampled in vivo (11 cases) and at necropsy (10 cases). A commercial fluorescent polyclonal antiserum of feline origin reacting with FIPV and cross reacting with transmissible gastroenteritis virus and canine coronavirus was used. Eleven cats with ascites of a different origin were used as negative controls. The direct immunofluorescence test was 97 per cent reliable (31 cases of 32) and can be used in routine diagnosis.

[1]  Whitenack Dl,et al.  Feline infectious peritonitis with spinal cord involvement in two cats. , 1975 .

[2]  D. Whitenack,et al.  Feline infectious peritonitis with spinal cord involvement in two cats. , 1975, Journal of the American Veterinary Medical Association.

[3]  Ingersoll Jd,et al.  Identification of viral antigens that induce antibody responses on exposure to coronaviruses. , 1988 .

[4]  G. Renzoni,et al.  Ulteriori rilievi sulla peritonite infettiva del gatto (FIP). , 1985 .

[5]  N. Pedersen,et al.  An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. , 1981, American journal of veterinary research.

[6]  K. Fujiwara,et al.  Systemic vascular lesions in feline infectious peritonitis. , 1977, Nihon juigaku zasshi. The Japanese journal of veterinary science.

[7]  G. Renzoni,et al.  Osservazioni anatomo-istopatologiche in corso di peritonite infettiva del gatto e rilievi patogenetici. , 1984 .

[8]  D. Wylie,et al.  Identification of viral antigens that induce antibody responses on exposure to coronaviruses. , 1988, American Journal of Veterinary Research.

[9]  C. E. Gilmore,et al.  Naturally occurring feline infectious peritonitis: signs and clinical diagnosis. , 1971, Journal of the American Veterinary Medical Association.

[10]  Doherty Mj.  Ocular manifestations of feline infectious peritonitis. , 1971 .

[11]  D. Wylie,et al.  Comparison of serologic assays for measurement of antibody response to coronavirus in cats. , 1988, American journal of veterinary research.

[12]  J. Timoney,et al.  Feline infectious peritonitis. , 1976, The Veterinary clinics of North America.

[13]  F. Scott,et al.  Laboratory diagnosis of feline infectious peritonitis. , 1980 .

[14]  J. Strandberg,et al.  Extraperitoneal Lesions in Feline Infectious Peritonitis , 1972, Veterinary pathology.

[15]  Griesemer Ra,et al.  Feline infectious peritonitis: review of gross and histopathologic lesions. , 1971 .

[16]  Ingersoll Jd,et al.  Comparison of serologic assays for measurement of antibody response to coronavirus in cats. , 1988 .

[17]  Z. Sagan.  [Laboratory diagnosis]. , 1971, Pielegniarka i polozna.

[18]  N. Pedersen.  Virologic and immunologic aspects of feline infectious peritonitis virus infection. , 1987, Advances in experimental medicine and biology.

[19]  J. Barlough.  Cats, coronaviruses and coronavirus antibody tests , 1985, The Journal of Small Animal Practice.

[20]  F. Scott,et al.  Pathogenesis of feline infetious peritonitis: pathologic changes and immunofluorescence. , 1981, American journal of veterinary research.