Analysis of macromolecule resonances in 1H NMR spectra of human brain

Macromolecule resonances underlying metabolites in 1H NMR spectra were investigated in temporal lobe biopsy tissue from epilepsy patients and from localized 1H spectra of the brains of healthy volunteers. The 1H NMR spectrum of brain tissue was cornpared with that of cytosol and dialyzed cytosol after removal of low molecular weight molecules (4500 daltons) at 8.4 and 2.1 Tesla. The assignment of specific resonances to macromolecules in 2.1 Tesla, short‐ TE, localized human brain 1H NMR spectra in vivo was made on the basis of a J‐editing method using the spectral parameters (δ, J) and connectivities determined from 2D experiments in vitro. Two prominent corinectivities associated with macromolecules in vitro (0.93–2.05 δ and 1.6–3.00 δ) were also detected in vivo by the J‐editing method. Advantage was taken of the large difference in measured T1 relaxation times between macromolecule and metabolite resonances in the brain spectrum to acquire ‘metabolite‐nulled’ macromolecule spectra. These spectra appear identical to the spectra of macromolecules isolated in vitro.

[1]  Takashi Ogino,et al.  Characterization of macromolecule resonances in the 1H NMR spectrum of rat brain , 1993, Magnetic resonance in medicine.

[2]  S R Williams,et al.  Quantitative analysis of 1H NMR detected proteins in the rat cerebral cortex in vivo and in vitro , 1993, NMR in biomedicine.

[3]  R. Mattson,et al.  Localized 1H NMR measurements of gamma-aminobutyric acid in human brain in vivo. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[4]  K. Brindle,et al.  The appearance of neutral lipid signals in the 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets , 1993, Magnetic resonance in medicine.

[5]  G. Barker,et al.  Detection of myelin breakdown products by proton magnetic resonance spectroscopy , 1993, The Lancet.

[6]  D. Paty,et al.  Magnetic resonance spectroscopy of multiple sclerosis: in-vivo detection of myelin breakdown products , 1993, The Lancet.

[7]  A. Bizzi,et al.  Absolute Quantitation of Short TE Brain 1H‐MR Spectra and Spectroscopic Imaging Data , 1993, Journal of computer assisted tomography.

[8]  H. Kauczor,et al.  3D TOF MR Angiography of Cerebral Arteriovenous Malformations after Radiosurgery , 1993, Journal of computer assisted tomography.

[9]  C T Moonen,et al.  Short echo time proton MR spectroscopic imaging. , 1993, Journal of computer assisted tomography.

[10]  R. Gruetter,et al.  Detection and assignment of the glucose signal in 1h nmr difference spectra of the human brain , 1992, Magnetic resonance in medicine.

[11]  George Fein,et al.  Proton magnetic resonance spectroscopy of human brain: Applications to normal white matter, chronic infarction, and MRI white matter signal hyperintensities , 1992, Magnetic resonance in medicine.

[12]  R. Kauppinen,et al.  Contribution of cytoplasmic polypeptides to the 1H NMR spectrum of developing rat cerebral cortex , 1992, Magnetic resonance in medicine.

[13]  R. Kauppinen,et al.  Detection of Mobile Proteins by Proton Nuclear Magnetic Resonance Spectroscopy in the Guinea Pig Brain Ex Vivo and Their Partial Purification , 1992, Journal of neurochemistry.

[14]  Rolf Gruetter,et al.  Fast, noniterative shimming of spatially localized signals. In vivo analysis of the magnetic field along axes , 1992 .

[15]  B D Ross,et al.  Metabolic disorders of the brain in chronic hepatic encephalopathy detected with H-1 MR spectroscopy. , 1992, Radiology.

[16]  O. Henriksen,et al.  In vivo relaxation of N-acetyl-aspartate, creatine plus phosphocreatine, and choline containing compounds during the course of brain infarction: a proton MRS study. , 1992, Magnetic resonance imaging.

[17]  J. Frahm,et al.  Localized proton NMR spectroscopy of brain tumors using short-echo time STEAM sequences. , 1991, Journal of computer assisted tomography.

[18]  K. Behar,et al.  Assignment of resonances in the 1H spectrum of rat brain by two‐dimensional shift correlated and j‐resolved NMR spectroscopy , 1991, Magnetic resonance in medicine.

[19]  P A Narayana,et al.  In vivo proton magnetic resonance spectroscopy studies of human brain. , 1991, Magnetic resonance imaging.

[20]  J. Alger,et al.  High‐field proton magnetic resonance spectroscopy of human cerebrum obtained during surgery for epilepsy , 1989, Neurology.

[21]  J. Frahm,et al.  Localized proton NMR spectroscopy in different regions of the human brain in vivo. Relaxation times and concentrations of cerebral metabolites , 1989, Magnetic resonance in medicine.

[22]  A. Benabid,et al.  In vivo 1H NMR spectroscopy of an intracerebral glioma in the rat , 1989, Magnetic resonance in medicine.

[23]  R. Freeman,et al.  Selective excitation with the DANTE sequence. The baseline syndrome , 1989 .

[24]  J. Alger,et al.  High‐Resolution Proton Magnetic Resonance Spectroscopy of Rabbit Brain: Regional Metabolite Levels and Postmortem Changes , 1988, Journal of neurochemistry.

[25]  R G Shulman,et al.  Spatially localized 1H NMR spectra of metabolites in the human brain. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[26]  M. Bárány,et al.  Observation of the terminal methyl group in fatty acids of the linolenic series by a new 1H NMR pulse sequence providing spectral editing and solvent suppression. Application to excised frog muscle and rat brain. , 1986, Biochemistry.

[27]  P E Wright,et al.  Assignment of methylene proton resonances in NMR spectra of embryonic and transformed cells to plasma membrane triglyceride. , 1986, The Journal of biological chemistry.

[28]  Roger J. Ordidge,et al.  Image-selected in Vivo spectroscopy (ISIS). A new technique for spatially selective nmr spectroscopy , 1986 .

[29]  D. Rothman,et al.  Phase cycling of composite refocusing pulses to eliminate dispersive refocusing magnetization , 1985 .

[30]  R G Shulman,et al.  1H homonuclear editing of rat brain using semiselective pulses. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[31]  C Arús,et al.  Proton nuclear magnetic resonance spectra of excised rat brain. Assignment of resonances. , 1985, Physiological chemistry and physics and medical NMR.

[32]  R G Shulman,et al.  Homonuclear 1H double-resonance difference spectroscopy of the rat brain in vivo. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[33]  D. Hoult,et al.  Selective population inversion in NMR , 1984, Nature.

[34]  P. J. Hore,et al.  Solvent suppression in Fourier transform nuclear magnetic resonance , 1983 .

[35]  R G Shulman,et al.  High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[36]  S. Martin,et al.  Molecular flexibility in microtubule proteins: proton nuclear magnetic resonance characterization. , 1983, Biochemistry.

[37]  R. R. Ernst,et al.  Two‐dimensional spectroscopy. Application to nuclear magnetic resonance , 1976 .