Antibacterial activity of various self-etching adhesive systems against oral streptococci.

The antibacterial properties of self-etching adhesive systems constitute an important issue in operative dentistry, since viable bacteria can still be present after cavity preparation. The current study evaluated the antibacterial activity of five one-step self-etching adhesives (SEAs) and four self-etching primers (SEPs) against oral streptococci. Clearfil S3 (S3), One-Up Bond F Plus (OU), Futurabond NR (FB), GBond (GB), Xeno IV (X4), Clearfil SE Bond (SE), Clearfil Protect Bond (PB), Adper SE Plus (AS) and AdheSE (AD) were tested for antibacterial activity against five streptococci species: S oralis, S sanguinis, S cricetus, S mutans and S sobrinus. Chlorhexidine (0.12%) and phosphoric acid (37%) gel were used as control. The agar diffusion test method was used. Plates containing BHI agar and 300 uL of bacterial cell suspension (0.5 MacFarland) were prepared. Holes 6 mm in diameter were made and partially filled with bacteriological agar. Then, 10 uL of each SEA or SEP was dropped and the plates were incubated under microaerophilic atmosphere at 37 degrees C for 48 hours and the diameter of each halo was registered. The results were analyzed by two-way ANOVA and Tukey test. PB exhibited the most effective antibacterial activity against oral streptococci. The performances of SE and FB were similar or better than chlorhexidine for all bacteria. S3, X4, AS, AD, OU and GB showed significantly lower inhibition values. Among the species tested, S oralis was the most sensitive to all self-etching adhesive systems; on the other hand, S cricetus, S mutans and S sobrinus were more resistant. Among the self-etching adhesive systems evaluated, Clearfil Protect Bond exhibited the most effective antibacterial activity against oral streptococci.

[1]  J. Hebling,et al.  Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria. , 2008, Journal of dentistry.

[2]  M. Giannini,et al.  Long-term TEM analysis of the nanoleakage patterns in resin-dentin interfaces produced by different bonding strategies. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[3]  E. Swift,et al.  In vitro inhibition of bacterial growth using different dental adhesive systems. , 2007, Operative dentistry.

[4]  A. Bedran-Russo,et al.  Interfacial ultramorphology of single-step adhesives: nanoleakage as a function of time. , 2007, Journal of oral rehabilitation.

[5]  A. Bedran-Russo,et al.  Adhesive systems and secondary caries formation: Assessment of dentin bond strength, caries lesions depth and fluoride release. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[6]  E. Weiss,et al.  Antibacterial properties of self-etching dental adhesive systems. , 2007, Journal of the American Dental Association.

[7]  M. Peters,et al.  In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. , 2006, Dental materials : official publication of the Academy of Dental Materials.

[8]  L. Pimenta,et al.  In vitro evaluation of caries inhibition promoted by self-etching adhesive systems containing antibacterial agents. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[9]  M. Serra,et al.  Visual evaluation of in vitro cariostatic effect of restorative materials associated with dentifrices. , 2005, Brazilian dental journal.

[10]  P. Lambrechts,et al.  A Critical Review of the Durability of Adhesion to Tooth Tissue: Methods and Results , 2005, Journal of dental research.

[11]  R. Carvalho,et al.  Ultramorphological analysis of resin-dentin interfaces produced with water-based single-step and two-step adhesives: nanoleakage expression. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[12]  Stefan Habelitz,et al.  The effect of a self-etching primer on the continuous demineralization of dentin. , 2004, European journal of oral sciences.

[13]  K. Hiller,et al.  Effect of dentin on the antibacterial activity of dentin bonding agents. , 2004, Journal of endodontics.

[14]  S. Imazato.  Antibacterial properties of resin composites and dentin bonding systems. , 2003, Dental materials : official publication of the Academy of Dental Materials.

[15]  G. Vanherle,et al.  Four-year Water Degradation of Total-etch Adhesives Bonded to Dentin , 2003, Journal of dental research.

[16]  D. Pashley,et al.  Aggressiveness of contemporary self-etching systems. I: Depth of penetration beyond dentin smear layers. , 2001, Dental materials : official publication of the Academy of Dental Materials.

[17]  Y. Torii,et al.  Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. , 2001, Journal of oral rehabilitation.

[18]  A. Kolokotronis,et al.  Antibacterial properties of dentin bonding systems, polyacid-modified composite resins and composite resins. , 2001, Journal of oral rehabilitation.

[19]  M. Kaga,et al.  In vivo Degradation of Resin-Dentin Bonds in Humans Over 1 to 3 Years , 2000, Journal of dental research.

[20]  N. Maeda,et al.  Evaluation of antibacterial activity of three dentin primers using an in vitro tooth model. , 1999, Operative dentistry.

[21]  J. Tagami,et al.  Long-term Durability of Dentin Bonds Made with a Self-etching Primer, in vivo , 1999, Journal of dental research.

[22]  S. Imazato,et al.  Antibacterial activity of proprietary self-etching primers. , 1998, American Journal of Dentistry.

[23]  M. Torii,et al.  Antibacterial activity of dentine primer containing MDPB after curing. , 1998, Journal of dentistry.

[24]  M. Torii,et al.  Incorporation of Antibacterial Monomer MDPB into Dentin Primer , 1997, Journal of dental research.

[25]  J. McCabe,et al.  Influence of Incorporation of Antibacterial Monomer on Curing Behavior of a Dental Composite , 1994, Journal of dental research.

[26]  M. Torii,et al.  Incorporation of Bacterial Inhibitor into Resin Composite , 1994, Journal of dental research.

[27]  J. Kanca.  Resin bonding to wet substrate. 1. Bonding to dentin. , 1992, Quintessence international.

[28]  A. Caputo,et al.  Antibacterial and mechanical properties of restorative materials combined with chlorhexidines. , 1983, Journal of oral rehabilitation.

[29]  C F Cox,et al.  Bacterial leakage around dental restorations: its effect on the dental pulp. , 1982, Journal of oral pathology.

[30]  N. Nakabayashi,et al.  The promotion of adhesion by the infiltration of monomers into tooth substrates. , 1982, Journal of biomedical materials research.