Reversibility of metabolic and morphological changes associated with chronic exposure of pancreatic islet β‐cells to fatty acids

Pancreatic β‐cells metabolise both lipid and glucose nutrients but chronic exposure (>24 h) to elevated fatty acid (FA) concentrations results in deleterious metabolic and morphological changes. The aims of this study were to assess the adaptive morphological, metabolic and secretory responses of islet β‐cells to exposure and removal of FA. Isolated mouse islets and INS‐1 β‐cells were exposed to oleate or palmitate (0.5 mM) or a 1:1 mixture of both FA for 48 h prior to a 24 h period without FA. Subsequent changes in lipid storage and composition (triglycerides, TG and phospholipids, PL), gene expression, β‐cell morphology and glucose‐stimulated insulin secretion (GSIS) were determined. Intracellular TG content increased during exposure to FA and was lower in cells subsequently incubated in FA‐free media (P < 0.05); TG storage was visible as oil red O positive droplets (oleate) by light microscopy or ‘splits’ (palmitate) by electron microscopy. Significant desaturation of β‐cell FA occurred after exposure to oleate and palmitate. After incubation in FA‐free media, there was differential handling of specific FA in TG, resulting in a profile that tended to revert to that of control cells. FA treatment resulted in elevated lipolysis of intracellular TG, increased FA oxidation and reduced GSIS. After incubation in FA‐free media, oxidation remained elevated but inhibition of FA oxidation with etomoxir (10 µM) had no effect on the improvement in GSIS. The β‐cell demonstrates metabolic flexibility as an adaptive response to ambient concentrations of FA. J. Cell. Biochem. 109: 683–692, 2010. © 2010 Wiley‐Liss, Inc.

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