Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae

Type 1 fimbriae of Escherichia coli are surface organelles which mediate binding to D-mannose-containing structures. By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the receptor specificity. Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae.

[1]  C. Brinton.  The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. , 1965, Transactions of the New York Academy of Sciences.

[2]  D. Old.  Inhibition of the interaction between fimbrial haemagglutinins and erythrocytes by D-mannose and other carbohydrates. , 1972, Journal of general microbiology.

[3]  N. Sharon,et al.  Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors , 1977, Nature.

[4]  J. Duguid,et al.  Adhesive Properties of Enterobacteriaceae , 1980 .

[5]  A. Birch‐Andersen,et al.  Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae , 1980, Infection and immunity.

[6]  L. Hagberg,et al.  Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections , 1981, Infection and immunity.

[7]  N. Firon,et al.  Interaction of mannose-containing oligosaccharides with the fimbrial lectin of Escherichia coli. , 1982, Biochemical and biophysical research communications.

[8]  J. Kyhse-Andersen.  Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. , 1984, Journal of biochemical and biophysical methods.

[9]  P. Klemm.  The fimA gene encoding the type-1 fimbrial subunit of Escherichia coli. Nucleotide sequence and primary structure of the protein. , 1984, European journal of biochemistry.

[10]  B. Koellreutter,et al.  Oligomannoside-type glycopeptides inhibiting adhesion of Escherichia coli strains mediated by type 1 pili: preparation of potent inhibitors from plant glycoproteins , 1986, Infection and immunity.

[11]  P. Klemm,et al.  Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli. , 1986, The EMBO journal.

[12]  L. Maurer,et al.  Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili , 1987, Journal of bacteriology.

[13]  E. Beachey,et al.  Identification of two ancillary subunits of Escherichia coli type 1 fimbriae by using antibodies against synthetic oligopeptides of fim gene products , 1987, Journal of bacteriology.

[14]  E. Beachey,et al.  Hyperadhesive mutant of type 1-fimbriated Escherichia coli associated with formation of FimH organelles (fimbriosomes) , 1988, Infection and immunity.

[15]  E. Beachey,et al.  Conservation of the D-mannose-adhesion protein among type 1 fimbriated members of the family Enterobacteriaceae , 1988, Nature.

[16]  M. Hanson,et al.  Purification of the Escherichia coli type 1 pilin and minor pilus proteins and partial characterization of the adhesin protein , 1988, Journal of bacteriology.

[17]  K. Krogfelt,et al.  Investigation of minor components of Escherichia coli type 1 fimbriae: protein chemical and immunological aspects. , 1988, Microbial pathogenesis.

[18]  J. Gershoni.  Protein blotting: a manual. , 1988, Methods of biochemical analysis.

[19]  T. Yamamoto,et al.  In vitro adherence of type 1-fimbriated uropathogenic Escherichia coli to human ureteral mucosa , 1989, Infection and immunity.

[20]  P. Klemm,et al.  Regulation of the phase switch controlling expression of type 1 fimbriae in Escherichia coli , 1989, Molecular microbiology.