Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object.

A method to reduce coherent noise in digital holographic phase contrast microscopy is proposed. By slightly shifting the specimen, a series of digital holograms with different coherent noise patterns is recorded. Each hologram is reconstructed individually, while the different phase tilts of the reconstructed complex amplitudes due to the specimen shifts are corrected in the hologram plane by using numerical parametric lens method. Afterward, the lateral displacements of the phase maps from different holograms are compensated in the image plane by using digital image registration method. Thus, all phase images have same distribution, but uncorrelated coherent noise patterns. By a proper averaging procedure, the coherent noise of phase contrast image is reduced significantly. The experimental results are given to confirm the proposed method.

[1]  Giancarlo Pedrini,et al.  Short-coherence digital microscopy by use of a lensless holographic imaging system. , 2002, Applied optics.

[2]  Catherine Yourassowsky,et al.  Digital holographic microscopy with reduced spatial coherence for three-dimensional particle flow analysis. , 2006, Applied optics.

[3]  Patrik Langehanenberg,et al.  Application of partially coherent light in live cell imaging with digital holographic microscopy , 2010 .

[4]  Etienne Cuche,et al.  Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation. , 2006, Applied optics.

[5]  Thomas Kreis.  Digital Recording and Numerical Reconstruction of Wave Fields , 2005 .

[6]  Chenggen Quan,et al.  Speckle noise reduction in digital holography by multiple holograms , 2007 .

[7]  Zahid Yaqoob,et al.  Speckle-field digital holographic microscopy , 2009 .

[8]  T. Nomura,et al.  Image quality improvement of digital holography by superposition of reconstructed images obtained by multiple wavelengths. , 2008, Applied optics.

[9]  Xiao-ou Cai.  Reduction of speckle noise in the reconstructed image of digital holography , 2010 .

[10]  Frank Dubois,et al.  Partial spatial coherence effects in digital holographic microscopy with a laser source. , 2004, Applied optics.

[11]  Patrik Langehanenberg,et al.  Phase noise optimization in temporal phase-shifting digital holography with partial coherence light sources and its application in quantitative cell imaging. , 2009, Applied optics.

[12]  Lu Rong,et al.  Speckle noise reduction in digital holography by use of multiple polarization holograms , 2010 .

[13]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[14]  E. Cuche,et al.  Characterization of microlenses by digital holographic microscopy. , 2006, Applied optics.

[15]  Daniel Velásquez Prieto,et al.  Reduction of speckle noise in digital holography by using digital image processing , 2005 .

[16]  Xin Kang,et al.  An effective method for reducing speckle noise in digital holography , 2008 .

[17]  Zainul Abdin Jaffery,et al.  Improvement of signal-to-noise ratio in digital holography using wavelet transform , 2008 .

[18]  Tomasz Kozacki,et al.  Digital reconstruction of a hologram recorded using partially coherent illumination , 2005 .

[19]  P. Ferraro,et al.  Tunable liquid microlens arrays in electrode-less configuration and their accurate characterization by interference microscopy. , 2009, Optics express.

[20]  Wolfgang Osten,et al.  Coherence effects in digital in-line holographic microscopy. , 2008, Journal of the Optical Society of America. A, Optics, image science, and vision.

[21]  U. Schnars,et al.  Direct recording of holograms by a CCD target and numerical reconstruction. , 1994, Applied optics.

[22]  Bahram Javidi,et al.  Reduction of speckle in digital holography by discrete Fourier filtering. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[23]  Xiao Wen,et al.  Long-working-distance synthetic aperture Fresnel off-axis digital holography. , 2009, Optics express.

[24]  Manuel Guizar-Sicairos,et al.  Efficient subpixel image registration algorithms. , 2008, Optics letters.

[25]  E. Cuche,et al.  Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. , 2005, Optics express.