Proteome profile of the developing maize (Zea mays L.) rachis

In this study, we performed the first high‐throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2‐DE and 2‐D LC, we identified 967 proteins. A 2‐D proteome reference map was established. Functional classification of identified proteins revealed that proteins involved in various cellular metabolisms, response to stimulus and transport, were the most abundant.

[1]  M. Ramsby,et al.  Differential detergent fractionation of eukaryotic cells. , 2011, Cold Spring Harbor protocols.

[2]  Lennart Martens,et al.  A guide to the Proteomics Identifications Database proteomics data repository , 2009, Proteomics.

[3]  Bindu Nanduri,et al.  Comparative Proteomic Analysis of Listeria monocytogenes Strains F2365 and EGD , 2008, Applied and Environmental Microbiology.

[4]  K. Klepzig,et al.  Global and comparative protein profiles of the pronotum of the southern pine beetle, Dendroctonus frontalis , 2008, Insect molecular biology.

[5]  Christophe Perin,et al.  Phylogenomics of plant genomes: a methodology for genome-wide searches for orthologs in plants , 2008, BMC Genomics.

[6]  Chinling Wang,et al.  Improved solubilization of surface proteins from Listeria monocytogenes for 2‐DE , 2007, Electrophoresis.

[7]  Nan Wang,et al.  AgBase: a functional genomics resource for agriculture , 2006, BMC Genomics.

[8]  Sixue Chen,et al.  Characterization of the maize xylem sap proteome. , 2006, Journal of proteome research.

[9]  R. E. Sharp,et al.  Cell Wall Proteome in the Maize Primary Root Elongation Zone. I. Extraction and Identification of Water-Soluble and Lightly Ionically Bound Proteins1 , 2005, Plant Physiology.

[10]  Bindu Nanduri,et al.  Proteomic analysis using an unfinished bacterial genome: The effects of subminimum inhibitory concentrations of antibiotics on Mannheimia haemolytica virulence factor expression , 2005, Proteomics.

[11]  P. Schnable,et al.  The accumulation of abundant soluble proteins changes early in the development of the primary roots of maize (Zea mays L.) , 2005, Proteomics.

[12]  Cathy H. Wu,et al.  The Universal Protein Resource (UniProt) , 2004, Nucleic Acids Res..

[13]  Myriam Ferro,et al.  Identification of New Intrinsic Proteins in Arabidopsis Plasma Membrane Proteome*S , 2004, Molecular & Cellular Proteomics.

[14]  O. Langella,et al.  A two-dimensional proteome map of maize endosperm. , 2004, Phytochemistry.

[15]  Vasant G Honavar,et al.  A Proteomic Analysis of Maize Chloroplast Biogenesis1 , 2004, Plant Physiology.

[16]  Kent Vander Velden,et al.  The proteome of maize leaves: Use of gene sequences and expressed sequence tag data for identification of proteins with peptide mass fingerprints , 2001, Electrophoresis.

[17]  A. Krogh,et al.  Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. , 2001, Journal of molecular biology.

[18]  M. Ashburner,et al.  Gene Ontology: tool for the unification of biology , 2000, Nature Genetics.

[19]  M. Ramsby,et al.  Differential detergent fractionation of eukaryotic cells. Analysis by two-dimensional gel electrophoresis. , 1999, Methods in molecular biology.

[20]  W. Hurkman,et al.  Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. , 1986, Plant physiology.